[长江流域不同土地利用类型沼泽地的土壤微生物和理化性质及其对钉螺分布的影响]

H He, J Zhou, Z Gao, Z Lu, R Dai, X Hu, H Pang
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引用次数: 0

摘要

研究目的研究长江流域不同土地利用类型沼泽地土壤微生物群落、理化性质及钉螺分布情况,探讨土壤微生物和理化性质对钉螺分布的影响,为长江流域沼泽地钉螺控制和血吸虫病防治提供参考:方法:2021 年 4 月,在长江流域沿岸选择了四种土地利用类型的沼泽地,包括杨树林-农作物综合种植区、芦苇区、农业耕地和沟渠。调查了不同土地利用类型沼泽地中蜗牛的分布和土壤理化性质,并对土壤中细菌 16S 核糖体 RNA(16S rRNA)基因 V3 至 V4 区、真菌内转录间隔-1(ITS1)基因和藻类核酮糖-二磷酸羧化酶(rbcL)基因进行了高通量测序。比较了不同土地利用类型的沼泽地中活蜗牛框架的出现情况和活蜗牛的密度。采用皮尔逊相关分析法研究了土壤微生物和理化性质与活螺密度的关系,并采用方差分区分析法评估了土壤微生物和理化性质对活螺密度的贡献:在四种土地利用类型的沼泽地中,沟渠中活蜗牛的出现率[(4.94 ± 2.14)%]和活蜗牛的密度[(0.070 ± 0.026) 蜗牛/0.1 m2]最高,农耕地中活蜗牛的出现率[(1.23 ± 1.23)%]和活蜗牛的密度[(0.016 ± 0.019) 蜗牛/0.1 m2]最低。在四种土地利用类型的土壤中,共检测到 2 门、5 纲、8 目、9 科、11 属藻类,其中叶绿藻门为优势门,假绿藻属为优势属。在四种土地利用类型的土壤中,共检测到 44 个菌门、134 个菌纲、281 个菌目、338 个菌科和 516 个菌属,其中蛋白质细菌和酸性细菌为优势菌门,未培养的酸性细菌、MND1、米特罗丝菌、卤虫和鞘氨醇单胞菌为优势菌属。在四种土地利用类型的土壤中,共检测到 11 门、41 类、108 目、223 科、408 属真菌,其中子囊菌门(Ascomycota)、担子菌门(Basidiomycota)和毛霉菌门(Mortierellomycota)的相对丰度较高,Cladorrhinum 属、Mortierella 属和 Humicola 属的相对丰度较高。皮尔逊相关分析表明,活蜗牛的密度与蛋白细菌的相对丰度(r = -0.965,P < 0.05)和土壤电子传导率(r = -0.962,P < 0.05)呈负相关,与土壤湿度(r = 0.951,P < 0.05)呈正相关。方差分配分析表明,土壤理化性质和微生物对活蜗牛密度的贡献率分别为 69% 和 10%:结论:长江流域沼泽地不同土地利用类型土壤中微生物群落的多样性存在差异,土壤的理化性质和微生物可能会影响有尾蜗牛的分布。
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[Soil microorganisms and physicochemical properties in marshlands along the Yangtze River basin at different types of land use and their effects on distribution of Oncomelania hupensis snails].

Objective: To investigating the microbial communities and physicochemical properties of soil and distribution of Oncomelania hupensis snails in marshlands along the Yangtze River basin at different types of land use, and to examine the effects of soil microorganisms and physicochemical properties on snail distribution, so as to provide insights into snail control and schistosomiasis prevention in marshland along the Yangtze River basin.

Methods: Marshlands with four types of land use were selected along the Yangtze River basin on April 2021, including poplar forest-crops integrated planting, reed areas, agricultural cultivation lands and ditches. The distribution of snails and physicochemical properties of soil were investigated in marshlands with different types of land use, and the V3 to V4 regions of the bacterial 16S ribosomal RNA (16S rRNA) gene, fungal internal transcribed spacer-1 (ITS1) gene and algal ribulose-bisphosphate carboxylase (rbcL) gene in soils were subjected to high-throughput sequencing. The occurrence of frames with living snails and density of living snails were compared in marshland with different types of land use. The associations of soil microorganisms and physicochemical properties with the density of living snails were examined using Pearson correlation analysis, and the contributions of soil microorganisms and physicochemical properties to the density of living snails were evaluated using variance partitioning analysis.

Results: In marshlands with four types of land use, the greatest occurrence of frames with living snails [(4.94 ± 2.14)%] and density of living snails [(0.070 ± 0.026) snails/0.1 m2] were seen in ditches, and the lowest were found in [(1.23 ± 1.23)%] agricultural cultivation lands [(0.016 ± 0.019) snails/0.1 m2]. A total of 2 phyla, 5 classes, 8 orders, 9 families and 11 genera of algae were detected in soils at four types of land use, with Chlorophyta as the dominant phylum and Pseudoneochloris as the dominant genus. A total of 44 phyla, 134 classes, 281 orders, 338 families and 516 genera of bacteria were detected in soils at four types of land use, with Proteobacteria and Acidobacteriota as the dominant phyla and uncultured Acidobacterium, MND1, Mitrospira, Haliangium and Sphingomonas as dominant genera. A total of 11 phyla, 41 classes, 108 orders, 223 families and 408 genera of fungi were detected in soils at four types of land use, with phyla Ascomycota, Basidiomycota and Mortierellomycota presenting high relative abundances and genera Cladorrhinum, Mortierella and Humicola presenting high relative abundances. Pearson correlation analysis revealed that the density of living snails correlated negatively with the relative abundance of Proteobacteria (r = -0.965, P < 0.05) and soil electronic conductivity (r = -0.962, P < 0.05) and positively with soil moisture (r = 0.951, P < 0.05). Variance partitioning analysis demonstrated that the physicochemical properties and microorganisms of soil contributed 69% and 10% to the density of living snails, respectively.

Conclusions: The diversity of microbial communities varies in soils at different types of land use in marshland along the Yangtze River basin, and the physicochemical properties and microorganisms of soils may affect the distribution of O. hupensis snails.

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来源期刊
中国血吸虫病防治杂志
中国血吸虫病防治杂志 Medicine-Medicine (all)
CiteScore
1.30
自引率
0.00%
发文量
7021
期刊介绍: Chinese Journal of Schistosomiasis Control (ISSN: 1005-6661, CN: 32-1374/R), founded in 1989, is a technical and scientific journal under the supervision of Jiangsu Provincial Health Commission and organised by Jiangsu Institute of Schistosomiasis Control. It is a scientific and technical journal under the supervision of Jiangsu Provincial Health Commission and sponsored by Jiangsu Institute of Schistosomiasis Prevention and Control. The journal carries out the policy of prevention-oriented, control-oriented, nationwide and grassroots, adheres to the tenet of scientific research service for the prevention and treatment of schistosomiasis and other parasitic diseases, and mainly publishes academic papers reflecting the latest achievements and dynamics of prevention and treatment of schistosomiasis and other parasitic diseases, scientific research and management, etc. The main columns are Guest Contributions, Experts‘ Commentary, Experts’ Perspectives, Experts' Forums, Theses, Prevention and Treatment Research, Experimental Research, The main columns include Guest Contributions, Expert Commentaries, Expert Perspectives, Expert Forums, Treatises, Prevention and Control Studies, Experimental Studies, Clinical Studies, Prevention and Control Experiences, Prevention and Control Management, Reviews, Case Reports, and Information, etc. The journal is a useful reference material for the professional and technical personnel of schistosomiasis and parasitic disease prevention and control research, management workers, and teachers and students of medical schools.    The journal is now included in important domestic databases, such as Chinese Core List (8th edition), China Science Citation Database (Core Edition), China Science and Technology Core Journals (Statistical Source Journals), and is also included in MEDLINE/PubMed, Scopus, EBSCO, Chemical Abstract, Embase, Zoological Record, JSTChina, Ulrichsweb, Western Pacific Region Index Medicus, CABI and other international authoritative databases.
期刊最新文献
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