Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2025-01-02 DOI: 10.16250/j.32.1915.2024194

S Bai, J Zhou, Y Dong, J Zhang, L Shi, K Yang

Objective: To construct a visual intelligent recognition model for Oncomelania hupensis robertsoni in Yunnan Province based on the EfficientNet-B4 model, and to evaluate the impact of data augmentation methods and model hyperparameters on the recognition of O. hupensis robertsoni.Methods: A total of 400 O. hupensis robertsoni and 400 Tricula snails were collected from Yongsheng County, Yunnan Province in June 2024, and snail images were captured following identification and classification of 300 O. hupensis robertsoni and 300 Tricula snails. A total of 925 O. hupensis robertsoni images and 1 062 Tricula snail images were collected as a dataset and divided into a training set and a validation set at a ratio of 8:2, while 352 images captured from the remaining 100 O. hupensis robertsoni and 354 images from the remaining 100 Tricula snails served as an external test set. All acquired images were subjected to preprocessing, including cropping and resizing. Three data augmentation approaches were employed, including baseline, Mixup and Gaussian blurring, and model hyperparameters included two optimization algorithms of adaptive moment estimation (Adam) and stochastic gradient descent (SGD), two loss functions of focal loss and cross entropy loss, and two learning rate decay strategies of cosine annealing and multi-step. The intelligent recognition models of O. hupensis robertsoni and Tricula snails were constructed based on the EfficientNet-B4 model, and 7 training strategy groups were generated by combinations of different data augmentation approaches and hyperparameters. The performance of intelligent recognition models was tested with external test sets, and evaluated with accuracy, precision, recall, F1 score, loss, Youden's index, and the area under the receiver operating characteristic curve (AUC) under different training strategies.Results: The variation of loss values was comparable among intelligent recognition models with different data augmentation approaches. The Group 4 model constructed with Mixup and Gaussian blurring data augmentation approaches showed the optimal performance, with an accuracy of 90.38%, precision of 90.07%, F1 score of 89.44%, Youden's index of 0.81 and AUC of 0.961 in the external test set. The accuracy of models using the SGD optimizer reduced by 29.16% as compared to those using the Adam optimizer (χ2 = 81.325, P < 0.001), and the accuracy of models using the cross entropy loss function reduced by 0.80% as compared to the Group 4 model (χ2 = 3.147, P > 0.05), while the accuracy of models using the multi-step learning rate decay strategy increased by 0.65% as compared to the Group 4 model (χ2 = 0.208, P > 0.05). In addition, the model with the baseline + Mixup + Gaussianblurring data augmentation approach and hype

目的：构建基于EfficientNet-B4模型的云南钉螺视觉智能识别模型，并评价数据增强方法和模型超参数对钉螺识别的影响。方法：于2024年6月在云南省永胜县采集罗氏钉螺400只、三角螺400只，对其中的罗氏钉螺300只、三角螺300只进行鉴定分类，采集钉螺图像。选取925张罗氏湖北钉螺图像和1 062张三角螺图像作为数据集，按8:2的比例划分为训练集和验证集，剩余100张罗氏湖北钉螺图像中的352张和剩余100张三角螺图像中的354张作为外部测试集。所有获得的图像进行预处理，包括裁剪和调整大小。采用基线、Mixup和高斯模糊三种数据增强方法，模型超参数包括自适应矩估计（Adam）和随机梯度下降（SGD）两种优化算法，焦点损失和交叉熵损失两种损失函数，余弦退火和多步学习率衰减策略。基于EfficientNet-B4模型构建了robertensis robertsoni和Tricula蜗牛的智能识别模型，并结合不同的数据增强方法和超参数生成了7个训练策略组。采用外部测试集对智能识别模型的性能进行测试，并对不同训练策略下智能识别模型的准确率、精密度、召回率、F1分数、损失、约登指数和接收者工作特征曲线下面积（AUC）进行评价。结果：不同数据增强方法的智能识别模型的损失值变化具有可比性。采用Mixup和高斯模糊数据增强方法构建的第4组模型表现出最优的性能，外部测试集的准确率为90.38%，精密度为90.07%，F1得分为89.44%，约登指数为0.81，AUC为0.961。使用SGD优化器的模型的准确率比使用Adam优化器的模型降低了29.16% (χ2 = 81.325, P < 0.001)，使用交叉熵损失函数的模型的准确率比第4组模型降低了0.80% (χ2 = 3.147, P > 0.05)，而使用多步学习率衰减策略的模型的准确率比第4组模型提高了0.65% （χ2 = 0.208, P > 0.05）。此外，采用baseline + Mixup + Gaussianblurring数据增强方法和Adam优化器、焦点损失函数和多步学习率衰减策略的超参数模型表现出最高的性能，准确率为91.03%，精密度为91.97%，召回率为88.11%，F1分数为90.00%，外部测试集的Youden指数为0.82，AUC值为0.969。结论：基于EfficientNet-B4模型的罗氏钉螺智能识别模型可准确识别云南省罗氏钉螺和三尾螺。

{"title":"[Construction of a visual intelligent identification model for Oncomelania hupensis robertsoni in Yunnan Province based on the EfficientNet-B4 model].","authors":"S Bai, J Zhou, Y Dong, J Zhang, L Shi, K Yang","doi":"10.16250/j.32.1915.2024194","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024194","url":null,"abstract":"Objective: To construct a visual intelligent recognition model for Oncomelania hupensis robertsoni in Yunnan Province based on the EfficientNet-B4 model, and to evaluate the impact of data augmentation methods and model hyperparameters on the recognition of O. hupensis robertsoni.Methods: A total of 400 O. hupensis robertsoni and 400 Tricula snails were collected from Yongsheng County, Yunnan Province in June 2024, and snail images were captured following identification and classification of 300 O. hupensis robertsoni and 300 Tricula snails. A total of 925 O. hupensis robertsoni images and 1 062 Tricula snail images were collected as a dataset and divided into a training set and a validation set at a ratio of 8:2, while 352 images captured from the remaining 100 O. hupensis robertsoni and 354 images from the remaining 100 Tricula snails served as an external test set. All acquired images were subjected to preprocessing, including cropping and resizing. Three data augmentation approaches were employed, including baseline, Mixup and Gaussian blurring, and model hyperparameters included two optimization algorithms of adaptive moment estimation (Adam) and stochastic gradient descent (SGD), two loss functions of focal loss and cross entropy loss, and two learning rate decay strategies of cosine annealing and multi-step. The intelligent recognition models of O. hupensis robertsoni and Tricula snails were constructed based on the EfficientNet-B4 model, and 7 training strategy groups were generated by combinations of different data augmentation approaches and hyperparameters. The performance of intelligent recognition models was tested with external test sets, and evaluated with accuracy, precision, recall, F1 score, loss, Youden's index, and the area under the receiver operating characteristic curve (AUC) under different training strategies.Results: The variation of loss values was comparable among intelligent recognition models with different data augmentation approaches. The Group 4 model constructed with Mixup and Gaussian blurring data augmentation approaches showed the optimal performance, with an accuracy of 90.38%, precision of 90.07%, F1 score of 89.44%, Youden's index of 0.81 and AUC of 0.961 in the external test set. The accuracy of models using the SGD optimizer reduced by 29.16% as compared to those using the Adam optimizer (χ2 = 81.325, P < 0.001), and the accuracy of models using the cross entropy loss function reduced by 0.80% as compared to the Group 4 model (χ2 = 3.147, P > 0.05), while the accuracy of models using the multi-step learning rate decay strategy increased by 0.65% as compared to the Group 4 model (χ2 = 0.208, P > 0.05). In addition, the model with the baseline + Mixup + Gaussianblurring data augmentation approach and hype","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"555-561"},"PeriodicalIF":0.0,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Progress of researches on mosquito salivary allergens]. 蚊虫唾液过敏原的研究进展

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-24 DOI: 10.16250/j.32.1915.2024114

S Jiang, X Zang, W Wang, S Hong

There are multiple bioactive substances in the mosquito saliva, most of which are allergic to humans. Previous studies have demonstrated that mosquito bites may induce allergic reactions mediated by B and T lymphocytes, resulting in a reduction in the quality of life and even death among patients. To date, 11 salivary allergens and 8 non-salivary allergens have been characterized in mosquitoes. Nevertheless, there is still lack of highly sensitive, highly specific and safe tools for diagnosis of mosquito bites-induced allergy, and the difficulty in obtaining natural mosquito salivary allergens results in failure in widespread applications of immunotherapy for mosquito bites-induced allergy. This review provides an overview of the allergic symptoms of mosquito bites and underlying mechanisms, and mosquito salivary allergens that have been characterized and registered in the systematic allergen nomenclature website.

蚊子唾液中含有多种生物活性物质，大部分对人类过敏。既往研究表明，蚊虫叮咬可引起B淋巴细胞和T淋巴细胞介导的过敏反应，使患者的生活质量下降，甚至死亡。迄今为止，已在蚊子中鉴定出11种唾液过敏原和8种非唾液过敏原。然而，目前仍缺乏高灵敏度、高特异性和安全的蚊虫叮咬致过敏诊断工具，且难以获得天然蚊虫唾液过敏原，导致蚊虫叮咬致过敏免疫治疗无法广泛应用。本文综述了蚊虫叮咬的过敏症状及其机制，以及在系统过敏原命名网站上已被鉴定和登记的蚊子唾液过敏原。

引用次数: 0

[Application of artificial intelligence in parasitic diseases and parasitology]. [人工智能在寄生虫病和寄生虫学中的应用]

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-24 DOI: 10.16250/j.32.1915.2024279

Y Zhou

The rapid development of artificial intelligence poses a huge impact on health and has become a core driving force for the new generation of the scientific and technological revolution in the field of healthcare. Recently, artificial intelligence has been gradually applied in the field of parasitic diseases and parasitology, including disease diagnosis, prognosis prediction, prediction of transmission risk, intelligent identification of vectors and intermediate hosts, and disease prevention and control, which facilitates the progress towards elimination of parasitic diseases. In addition, artificial intelligence provides highly efficient tools and approaches for healthcare workers and researchers. This comment mainly reviews the application of artificial intelligence in the fields of parasitic diseases and parasitology.

人工智能的快速发展对健康产生了巨大的影响，已成为医疗保健领域新一代科技革命的核心动力。近年来，人工智能已逐步应用于寄生虫病和寄生虫学领域，包括疾病诊断、预后预测、传播风险预测、媒介和中间宿主智能识别、疾病预防和控制等，促进了寄生虫病消除的进展。此外，人工智能为医护人员和研究人员提供了高效的工具和方法。本文主要综述了人工智能在寄生虫病和寄生虫学领域的应用。

引用次数: 0

[Prediction of areas of Oncomelania hupensis snail spread in Anhui Province based on five machine learning models]. [基于5种机器学习模型的安徽省钉螺传播区域预测]。

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-19 DOI: 10.16250/j.32.1915.2024085

F Gao

Objective: To predict the areas of Oncomelania hupensis snail spread in Anhui Province from 1977 to 2023 using machine learning models, and to compare the effectiveness of different machine learning models for prediction of areas of O. hupensis snail spread, so as to provide insights into investigating the trends in areas of O. hupensis snail spread.

Methods: Data pertaining to O. hupensis snail spread in Anhui Province from 1977 to 2023 were collected and a database was created. Five machine learning models were created using the software Matlab R2019b, including support vector regression (SVR), nonlinear autoregressive (NAR) neural network, back propagation (BP) neural network, gated recurrent unit (GRU) neural network and long short-term memory (LSTM) neural network models, and the model fitting effect was evaluated with mean absolute error (MAE), root mean squared error (RMSE) and coefficient of determination (R²). Following model training, the areas of O. hupensis snail spread were predicted in Anhui Province from 2024 to 2030.

Results: The cumulative areas of O. hupensis snail spread were 40 241.32 hm² in Anhui Province from 1977 to 2023, and the area of O. hupensis snail spread varied greatly among years, with a periodic peak every 4 to 6 years. The fitting curves of SVR, NAR neural network, BP neural network, GRU neural network and LSTM neural network models were increasingly closer to the real curves for areas of O. hupensis snail spread in Anhui Province. The trends in areas of O. hupensis snail spread in Anhui Province from 2024 to 2030 appeared approximately "M"-shaped curves by SVR and NAR neural network models, approximately "W"-shaped curves by BP and GRU neural network models, and a unimodal conical curve by the LSTM neural network model. The LSTM neural network model had the best effect for predicting areas of O. hupensis snail spread in Anhui Province, with the RMSE of 1 277 480, MAE of 797 422 and R² of 0.978 9, respectively.

Conclusions: Among the five models, The LSTM neural network model has a high efficiency for predicting areas of O. hupensis snail spread in Anhui Province, which may serve as a tool to investigate the trends in areas of O. hupensis snail spread.

目的：利用机器学习模型预测1977 - 2023年安徽省钉螺传播区域，并比较不同机器学习模型预测钉螺传播区域的有效性，为研究安徽省钉螺传播区域趋势提供依据。方法：收集安徽省1977 ~ 2023年血吸虫传播资料并建立数据库。利用Matlab R2019b软件建立支持向量回归（SVR）、非线性自回归（NAR）神经网络、反向传播（BP）神经网络、门控循环单元（GRU）神经网络和长短期记忆（LSTM）神经网络5个机器学习模型，并通过平均绝对误差（MAE）、均方根误差（RMSE）和决定系数（R2）对模型拟合效果进行评价。通过模型训练，预测了2024 - 2030年安徽省血吸虫传播面积。结果：1977 - 2023年安徽省累计发现湖北钉螺传播面积为40 241.32 hm2，年际差异较大，每4 ~ 6年出现一个周期高峰。SVR、NAR神经网络、BP神经网络、GRU神经网络和LSTM神经网络模型对安徽省钉螺传播面积的拟合曲线越来越接近真实曲线。基于SVR和NAR神经网络模型，安徽省2024 - 2030年湖北钉螺的传播趋势呈现近似“M”型曲线，基于BP和GRU神经网络模型呈现近似“W”型曲线，基于LSTM神经网络模型呈现单峰锥形曲线。LSTM神经网络模型对安徽省钉螺传播面积的预测效果最好，RMSE为1 277 480，MAE为797 422，R2为0.978 9。结论：在5个模型中，LSTM神经网络模型对安徽省钉螺传播区域的预测效率较高，可作为研究安徽省钉螺传播区域趋势的工具。

{"title":"[Prediction of areas of Oncomelania hupensis snail spread in Anhui Province based on five machine learning models].","authors":"F Gao","doi":"10.16250/j.32.1915.2024085","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024085","url":null,"abstract":"Objective: To predict the areas of Oncomelania hupensis snail spread in Anhui Province from 1977 to 2023 using machine learning models, and to compare the effectiveness of different machine learning models for prediction of areas of O. hupensis snail spread, so as to provide insights into investigating the trends in areas of O. hupensis snail spread.Methods: Data pertaining to O. hupensis snail spread in Anhui Province from 1977 to 2023 were collected and a database was created. Five machine learning models were created using the software Matlab R2019b, including support vector regression (SVR), nonlinear autoregressive (NAR) neural network, back propagation (BP) neural network, gated recurrent unit (GRU) neural network and long short-term memory (LSTM) neural network models, and the model fitting effect was evaluated with mean absolute error (MAE), root mean squared error (RMSE) and coefficient of determination (R2). Following model training, the areas of O. hupensis snail spread were predicted in Anhui Province from 2024 to 2030.Results: The cumulative areas of O. hupensis snail spread were 40 241.32 hm2 in Anhui Province from 1977 to 2023, and the area of O. hupensis snail spread varied greatly among years, with a periodic peak every 4 to 6 years. The fitting curves of SVR, NAR neural network, BP neural network, GRU neural network and LSTM neural network models were increasingly closer to the real curves for areas of O. hupensis snail spread in Anhui Province. The trends in areas of O. hupensis snail spread in Anhui Province from 2024 to 2030 appeared approximately \"M\"-shaped curves by SVR and NAR neural network models, approximately \"W\"-shaped curves by BP and GRU neural network models, and a unimodal conical curve by the LSTM neural network model. The LSTM neural network model had the best effect for predicting areas of O. hupensis snail spread in Anhui Province, with the RMSE of 1 277 480, MAE of 797 422 and R2 of 0.978 9, respectively.Conclusions: Among the five models, The LSTM neural network model has a high efficiency for predicting areas of O. hupensis snail spread in Anhui Province, which may serve as a tool to investigate the trends in areas of O. hupensis snail spread.","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"572-576"},"PeriodicalIF":0.0,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Prediction of potential geographic distribution of Oncomelania hupensis in Yunnan Province using random forest and maximum entropy models]. 基于随机森林和最大熵模型的云南钉螺潜在地理分布预测

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-12 DOI: 10.16250/j.32.1915.2024136

Z Zhang, C Du, Y Zhang, H Wang, J Song, J Zhou, L Wang, J Sun, M Shen, C Chen, H Jiang, J Yan, X Feng, W Wang, P Qian, J Xue, S Li, Y Dong

Objective: To predict the potential geographic distribution of Oncomelania hupensis in Yunnan Province using random forest (RF) and maximum entropy (MaxEnt) models, so as to provide insights into O. hupensis surveillance and control in Yunnan Province.Methods: The O. hupensis snail survey data in Yunnan Province from 2015 to 2016 were collected and converted into O. hupensis snail distribution site data. Data of 22 environmental variables in Yunnan Province were collected, including twelve climate variables (annual potential evapotranspiration, annual mean ground surface temperature, annual precipitation, annual mean air pressure, annual mean relative humidity, annual sunshine duration, annual mean air temperature, annual mean wind speed, ≥ 0 ℃ annual accumulated temperature, ≥ 10 ℃ annual accumulated temperature, aridity and index of moisture), eight geographical variables (normalized difference vegetation index, landform type, land use type, altitude, soil type, soil textureclay content, soil texture-sand content and soil texture-silt content) and two population and economic variables (gross domestic product and population). Variables were screened with Pearson correlation test and variance inflation factor (VIF) test. The RF and MaxEnt models and the ensemble model were created using the biomod2 package of the software R 4.2.1, and the potential distribution of O. hupensis snails after 2016 was predicted in Yunnan Province. The predictive effects of models were evaluated through cross-validation and independent tests, and the area under the receiver operating characteristic curve (AUC), true skill statistics (TSS) and Kappa statistics were used for model evaluation. In addition, the importance of environmental variables was analyzed, the contribution of environmental variables output by the models with AUC values of > 0.950 and TSS values of > 0.850 were selected for normalization processing, and the importance percentage of environmental variables was obtained to analyze the importance of environmental variables.Results: Data of 148 O. hupensis snail distribution sites and 15 environmental variables were included in training sets of RF and MaxEnt models, and both RF and MaxEnt models had high predictive performance, with both mean AUC values of > 0.900 and all mean TSS values and Kappa values of > 0.800, and significant differences in the AUC (t = 19.862, P < 0.05), TSS (t = 10.140, P < 0.05) and Kappa values (t = 10.237, P < 0.05) between two models. The AUC, TSS and Kappa values of the ensemble model were 0.996, 0.954 and 0.920, respectively. Independent data verification showed that the AUC, TSS and Kappa values of the RF model and the ensemble model were all 1, which still showed high performance in unknown data modeling, and the MaxEnt model showed poor

目的：利用随机森林（RF）和最大熵（MaxEnt）模型预测云南省可能发生的湖北钉螺地理分布，为云南省监测和防治提供依据。方法：收集云南省2015 - 2016年血吸虫调查资料，转化为血吸虫分布点资料。本文收集了云南省22个环境变量的数据，包括12个气候变量（年潜在蒸散量、年平均地表温度、年降水量、年平均气压、年平均相对湿度、年日照时数、年平均气温、年平均风速、≥0℃年积温、≥10℃年积温、干旱性和湿度指数）。8个地理变量（归一化差异植被指数、地貌类型、土地利用类型、海拔高度、土壤类型、土壤质地—粘土含量、土壤质地—砂含量和土壤质地—粉含量）和2个人口和经济变量（国内生产总值和人口）。采用Pearson相关检验和方差膨胀因子（variance inflation factor， VIF）检验筛选变量。利用r4.2.1软件中的biomod2软件包建立了RF和MaxEnt模型以及集合模型，预测了2016年后云南省湖北钉螺的潜在分布。通过交叉验证和独立检验评价模型的预测效果，采用受试者工作特征曲线下面积（AUC）、真技能统计量（TSS）和Kappa统计量进行模型评价。此外，对环境变量的重要性进行分析，选取AUC值为> 0.950、TSS值为> 0.850的模型对环境变量输出的贡献进行归一化处理，得到环境变量的重要性百分比，分析环境变量的重要性。结果:数据148 o . hupensis蜗牛分布站点和15个环境变量包含在训练集的射频和MaxEnt模型,射频和MaxEnt模型预测性能很高,平均AUC值> 0.900和所有的意思是TSS值和Kappa值> 0.800,和AUC显著差异(t = 19.862, P < 0.05), TSS (t = 10.140, P < 0.05)和k值(t = 10.237, P < 0.05)两个模型之间的关系。集合模型的AUC、TSS和Kappa值分别为0.996、0.954和0.920。独立数据验证表明，RF模型和集成模型的AUC、TSS和Kappa值均为1，在未知数据建模中仍然表现出较高的性能，而MaxEnt模型表现较差，24%（24/100）的建模结果中TSS和Kappa值为0。选取AUC值为> 0.950、TSS值为> 0.850的79个RF模型、38个MaxEnt模型及其集合模型的建模结果进行环境变量重要性评价。在RF模型、MaxEnt模型及其集合模型中，年日照时数（SSD）的重要性分别为32.989%、37.847%和46.315%，年平均相对湿度（RHU）的重要性分别为30.947%、15.921%和28.121%。重要的环境变量集中在RF模型的建模结果中，分散在MaxEnt模型的建模结果中，最集中在ensemble模型的建模结果中。RF模型预测2016年后湖北钉螺的潜在分布相对集中在云南省，而MaxEnt模型预测的潜在分布相对较大，集合模型预测的湖北钉螺分布多为RF和MaxEnt模型预测的联合分布。结论：RF模型和MaxEnt模型均能有效预测云南省湖北钉螺的潜在分布，为针对性防治提供依据。

{"title":"[Prediction of potential geographic distribution of Oncomelania hupensis in Yunnan Province using random forest and maximum entropy models].","authors":"Z Zhang, C Du, Y Zhang, H Wang, J Song, J Zhou, L Wang, J Sun, M Shen, C Chen, H Jiang, J Yan, X Feng, W Wang, P Qian, J Xue, S Li, Y Dong","doi":"10.16250/j.32.1915.2024136","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024136","url":null,"abstract":"Objective: To predict the potential geographic distribution of Oncomelania hupensis in Yunnan Province using random forest (RF) and maximum entropy (MaxEnt) models, so as to provide insights into O. hupensis surveillance and control in Yunnan Province.Methods: The O. hupensis snail survey data in Yunnan Province from 2015 to 2016 were collected and converted into O. hupensis snail distribution site data. Data of 22 environmental variables in Yunnan Province were collected, including twelve climate variables (annual potential evapotranspiration, annual mean ground surface temperature, annual precipitation, annual mean air pressure, annual mean relative humidity, annual sunshine duration, annual mean air temperature, annual mean wind speed, ≥ 0 ℃ annual accumulated temperature, ≥ 10 ℃ annual accumulated temperature, aridity and index of moisture), eight geographical variables (normalized difference vegetation index, landform type, land use type, altitude, soil type, soil textureclay content, soil texture-sand content and soil texture-silt content) and two population and economic variables (gross domestic product and population). Variables were screened with Pearson correlation test and variance inflation factor (VIF) test. The RF and MaxEnt models and the ensemble model were created using the biomod2 package of the software R 4.2.1, and the potential distribution of O. hupensis snails after 2016 was predicted in Yunnan Province. The predictive effects of models were evaluated through cross-validation and independent tests, and the area under the receiver operating characteristic curve (AUC), true skill statistics (TSS) and Kappa statistics were used for model evaluation. In addition, the importance of environmental variables was analyzed, the contribution of environmental variables output by the models with AUC values of > 0.950 and TSS values of > 0.850 were selected for normalization processing, and the importance percentage of environmental variables was obtained to analyze the importance of environmental variables.Results: Data of 148 O. hupensis snail distribution sites and 15 environmental variables were included in training sets of RF and MaxEnt models, and both RF and MaxEnt models had high predictive performance, with both mean AUC values of > 0.900 and all mean TSS values and Kappa values of > 0.800, and significant differences in the AUC (t = 19.862, P < 0.05), TSS (t = 10.140, P < 0.05) and Kappa values (t = 10.237, P < 0.05) between two models. The AUC, TSS and Kappa values of the ensemble model were 0.996, 0.954 and 0.920, respectively. Independent data verification showed that the AUC, TSS and Kappa values of the RF model and the ensemble model were all 1, which still showed high performance in unknown data modeling, and the MaxEnt model showed poor ","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"562-571"},"PeriodicalIF":0.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Incidence and risk factors of anemia among newly reported HIV/AIDS patients in Jiangsu Province in 2021]. [2021年江苏省新报告HIV/AIDS患者贫血发生率及危险因素分析]。

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-09 DOI: 10.16250/j.32.1915.2024174

Z Zhang, Q Sun, T Qiu, P Ding, B Wang, B Zhu

Objective: To investigate the incidence of anemia and evaluate the immune status among newly reported HIV/AIDS patients in Jiangsu Province in 2021, and to identify the risk factors of anemia among patients living with HIV infections.Methods: Newly reported HIV/AIDS patients in Jiangsu Province from January 1 to December 31, 2021 that were registered in China's National AIDS Comprehensive Control Information Management System were enrolled. Subjects' fresh whole blood samples were collected, and hemoglobin levels, CD4 and CD8 cell counts and HIV viral loads were measured. Anemia was defined according to hemoglobin levels, and the immunological parameters and HIV viral loads were compared between HIV-infected patients with and without anemia. The risk factors of anemia were identified among individuals living with HIV infections using univariate analysis and multivariate logistic regression analysis. In addition, subjects' CD4 cell counts one year following antiretroviral therapy (ART) were retrieved from China's National AIDS Comprehensive Control Information Management System, and compared between subjects with and without anemia.Results: A total of 635 newly diagnosed HIV/AIDS patients were reported in Jiangsu Province in 2021, including 544 males (85.67%) and 91 females (14.33%), and with ages of 15 to 83 years, and the overall incidence of anemia was 5.51% (35/635) among the study subjects. Men, individuals at ages of 45 years and lower and workers had relatively higher hemoglobin levels, with median hemoglobin levels of 156 (interquartile range, 22), 154 (interquartile range, 23) g/L and 162 (interquartile range, 19) g/L, respectively. The median baseline HIV viral load was 40 500.00 (interquartile range, 119 735.00) copies/mL among HIV-infected individuals with anemia and 29 754.00 (69 183.00) copies/mL among those without anemia (Z = -0.91, P = 0.31), and the median baseline CD4 and CD8 cell counts were significantly lower among HIV-infected individuals with anemia [166 (interquartile range, 143) cells/μL and 755 (653) cells/μL] than those without anemia [308 (253) cells/μL and 892 (638) cells/μL] (Z = -4.36 and -2.37, both P values < 0.05). The median CD4 cell counts remained lower among HIV-infected individuals with anemia than those without anemia [296 (interquartile range, 229) cells/μL vs. 457 (interquartile range, 313) cells/μL; Z = -3.71, P < 0.05] one year following ART, and the proportions of moderate and severe immunosuppression were significantly higher among HIV-infected individuals with anemia (40.00% and 17.14%) than those without anemia (21.00% and 9.33%) (χ2 = 10.37 and 8.79, both P values < 0.01). Univariate analysis showed a higher detection rate of anemia among female HIV-infected individuals than among males [odds ratio (OR) = 4.528, 95% confidence interval (CI): (3.811, 5.245), <

目的：了解江苏省2021年新报告HIV/AIDS患者贫血发生率及免疫状况，探讨HIV感染患者贫血的危险因素。方法：选取江苏省2021年1月1日至12月31日在中国艾滋病综合防治信息管理系统中登记的新报告艾滋病患者。采集受试者新鲜全血样本，测定血红蛋白水平、CD4和CD8细胞计数及HIV病毒载量。根据血红蛋白水平定义贫血，并比较有和无贫血的HIV感染者的免疫参数和HIV病毒载量。通过单因素分析和多因素logistic回归分析，确定HIV感染者贫血的危险因素。此外，从中国国家艾滋病综合控制信息管理系统中检索受试者在抗逆转录病毒治疗（ART）后一年的CD4细胞计数，并将有无贫血的受试者进行比较。结果：2021年江苏省共报告新诊断HIV/AIDS患者635例，其中男性544例（85.67%），女性91例（14.33%），年龄在15 ~ 83岁之间，研究对象贫血总体发生率为5.51%（35/635）。男性、45岁及以下的个体和工人的血红蛋白水平相对较高，血红蛋白水平的中位数分别为156（四分位数范围22）、154（四分位数范围23）和162（四分位数范围19）g/L。HIV-贫血感染者的基线HIV病毒载量中位数为40 500.00（四分位数范围，119 735.00）copies/mL，非贫血感染者的基线HIV病毒载量中位数为29 754.00 (69 183.00)copies/mL (Z = -0.91, P = 0.31)，且HIV-贫血感染者的CD4和CD8细胞计数中位数[166（四分位数范围，143）cells/μL和755 (653)cells/μL]显著低于非贫血感染者[308 (253)cells/μL和892 (638)cells/μL] （Z = -4.36和-2.37）。P值均< 0.05)。艾滋病毒感染的贫血患者CD4细胞计数中位数仍低于无贫血患者[296（四分位数范围，229）个细胞/μL vs. 457（四分位数范围，313）个细胞/μL；Z = -3.71, P < 0.05]，贫血hiv感染者出现中度和重度免疫抑制的比例（40.00%和17.14%）显著高于无贫血hiv感染者（21.00%和9.33%）（χ2 = 10.37、8.79，P值均< 0.01）。单因素分析显示，女性hiv感染者的贫血检出率高于男性[比值比(OR) = 4.528, 95%可信区间（CI）：（3.811, 5.245）， P < 0.001]， 45 ~ 60岁hiv感染者的贫血检出率高于男性[OR = 3.415, 95% CI: (1.191, 9.788), P = 0.022]和60岁及以上hiv感染者[OR = 5.820, 95% CI：（2.013, 16.826）， P < 0.001)与15 ~ 30岁以下人群相比，异性恋传播的hiv感染者感染率高于同质传播的hiv感染者[OR = 3.015, 95% CI: (1.423, 6.387), P = 0.004]，大学及以上文化程度的hiv感染者感染率低于小学文化程度的hiv感染者[OR = 0.103, 95% CI：（0.028, 0.386）， P < 0.001)，基线CD4细胞计数< 200细胞/μL的hiv感染者检出率高于基线CD4细胞计数≥200细胞/μL的hiv感染者[OR = 4.340, 95% CI: (2.165, 8.702), P < 0.001]， CD4/CD8细胞比值为0.208 ~ < 0.326 [OR = 0.232, 95% CI: (0.076, 0.711), P = 0.011]和0.516及以上的hiv感染者检出率较低[OR = 0.292, 95% CI：（0.104, 0.818）， P = 0.019)高于CD4/CD8细胞比值< 0.208的患者。多因素logistic回归分析发现，女性[OR = 4.945, 95% CI: (3.944, 5.946), P = 0.002]和CD4细胞计数< 200 cells/μL [OR = 3.597, 95% CI: (1.448, 8.937), P = 0.006]是新报告HIV/AIDS患者贫血的危险因素。结论：江苏省2021年新报告的HIV/AIDS患者中贫血发生率较低，且HIV感染者在基线和抗逆转录病毒治疗后1年的免疫状况较无贫血者差。女性和CD4细胞计数< 200 cells/μL是HIV感染者贫血的危险因素，建议针对女性HIV感染者和CD4细胞计数低的HIV感染者加强监测、随访和精准干预。

{"title":"[Incidence and risk factors of anemia among newly reported HIV/AIDS patients in Jiangsu Province in 2021].","authors":"Z Zhang, Q Sun, T Qiu, P Ding, B Wang, B Zhu","doi":"10.16250/j.32.1915.2024174","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024174","url":null,"abstract":"Objective: To investigate the incidence of anemia and evaluate the immune status among newly reported HIV/AIDS patients in Jiangsu Province in 2021, and to identify the risk factors of anemia among patients living with HIV infections.Methods: Newly reported HIV/AIDS patients in Jiangsu Province from January 1 to December 31, 2021 that were registered in China's National AIDS Comprehensive Control Information Management System were enrolled. Subjects' fresh whole blood samples were collected, and hemoglobin levels, CD4 and CD8 cell counts and HIV viral loads were measured. Anemia was defined according to hemoglobin levels, and the immunological parameters and HIV viral loads were compared between HIV-infected patients with and without anemia. The risk factors of anemia were identified among individuals living with HIV infections using univariate analysis and multivariate logistic regression analysis. In addition, subjects' CD4 cell counts one year following antiretroviral therapy (ART) were retrieved from China's National AIDS Comprehensive Control Information Management System, and compared between subjects with and without anemia.Results: A total of 635 newly diagnosed HIV/AIDS patients were reported in Jiangsu Province in 2021, including 544 males (85.67%) and 91 females (14.33%), and with ages of 15 to 83 years, and the overall incidence of anemia was 5.51% (35/635) among the study subjects. Men, individuals at ages of 45 years and lower and workers had relatively higher hemoglobin levels, with median hemoglobin levels of 156 (interquartile range, 22), 154 (interquartile range, 23) g/L and 162 (interquartile range, 19) g/L, respectively. The median baseline HIV viral load was 40 500.00 (interquartile range, 119 735.00) copies/mL among HIV-infected individuals with anemia and 29 754.00 (69 183.00) copies/mL among those without anemia (Z = -0.91, P = 0.31), and the median baseline CD4 and CD8 cell counts were significantly lower among HIV-infected individuals with anemia [166 (interquartile range, 143) cells/μL and 755 (653) cells/μL] than those without anemia [308 (253) cells/μL and 892 (638) cells/μL] (Z = -4.36 and -2.37, both P values < 0.05). The median CD4 cell counts remained lower among HIV-infected individuals with anemia than those without anemia [296 (interquartile range, 229) cells/μL vs. 457 (interquartile range, 313) cells/μL; Z = -3.71, P < 0.05] one year following ART, and the proportions of moderate and severe immunosuppression were significantly higher among HIV-infected individuals with anemia (40.00% and 17.14%) than those without anemia (21.00% and 9.33%) (χ2 = 10.37 and 8.79, both P values < 0.01). Univariate analysis showed a higher detection rate of anemia among female HIV-infected individuals than among males [odds ratio (OR) = 4.528, 95% confidence interval (CI): (3.811, 5.245), <","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"598-605"},"PeriodicalIF":0.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Physiological characteristics of Bacillus thuringiensis subspecies israelensis with double mutations of cwlE and sigK and its activity against larvae of Culex pipiens pallens]. [cwlE和sigK双突变苏云金芽孢杆菌以色列亚种的生理特性及其对淡色库蚊幼虫的活性]。

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-09 DOI: 10.16250/j.32.1915.2024189

L Huang, G Han, C Li, M Lin, N Zhang, J Xu

Objective: To investigate the physiological characteristics of Bacillus thuringiensis subspecies israelensis (Bti) with double mutations of cwlE and sigK genes and to assess the activity of Bti against larvae of Culex pipiens pallens under different external factors, so as to provide the theoretical evidence for the use of engineered bacteria of Bti for effective mosquito control.Methods: B. thuringiensis wild-type strain Bt-59 and Bt-59 strain with cwlE mutation [Bt-59 (ΔcwlE)] were cultured in nutrient broth media for 24 hours, and Bt-59 strains with sigK mutation [Bt-59 (ΔsigK)] and double mutations of cwlE and sigK [Bt-59 (ΔcwlE-sigK)] were cultured in nutrient broth media for 48 hours. Then, 5 μL of culture media were transferred to glass sides, and cell morphology and mother cell lysis were observed under an optical microscope. The optical densities of Bti strain culture media were measured at different time points of culture, and the growth curves of Bt-59, Bt-59 (ΔcwlE), Bt-59 (ΔsigK), and Bt-59 (ΔcwlE-sigK) strains were plotted. The differences in carbon source metabolism of four Bti strains were analyzed using the Biolog microplate culture method, and the metabolic activity of these strains was estimated with average well color development (AWCD). The fermentation media of these four Bti strains were diluted into final concentrations of 2.000, 1.000, 0.500, 0.250, and 0.125 μL/L, and the median lethal concentrations (LC50 values) of these four strains against the third instar larvae of Cx. pipiens pallens were determined. In addition, the fermentation media of Bti strains were processed as follows: pH adjusted to 5, 7 and 9; treated at 30, 40 ℃ and 50 ℃ for 12 hours; and exposed to irradiation with ultraviolet lights for 0 hour and 6 hours. Then, 20 third instar larvae of Cx. pipiens pallens were exposed to the above processed fermentation media at a final concentration of 1 μL/L in 200 mL of water at 26 ℃ for 24 hours, and the mosquito mortality was estimated to evaluate the effects of pH, temperature and ultraviolet irradiation on the larvicidal activity of four Bti strains.Results: The growth curves of the Bt-59 strain and its mutants shared a similar changing trend, and both experienced a stable phase 6 hours post-culture. Both spores and crystal proteins were found in Bt-59 and Bt-59 (ΔcwlE) cells, and only crystal proteins were found in Bt-59 (ΔsigK) and Bt-59 (ΔcwlE-sigK) cells. No lysis was found in the cell wall of the Bt-59 (ΔcwlE-sigK) strain, and the crystal protein was embedded in the mother cell. Biolog microplate culture assay showed that the AWCD values of four Bti strains showed a similar changing trend over time, and 33 carbon sources were found to be metabolized by all of the four strains,

目的：研究cwlE和sigK基因双突变苏云金芽孢杆菌以色列亚种（Bti）的生理特性，评价Bti在不同外界因素下对淡色库蚊幼虫的杀灭活性，为利用Bti工程菌进行有效灭蚊提供理论依据。方法：将苏云金芽孢杆菌野生型菌株Bt-59和cwlE突变菌株Bt-59 [Bt-59 （ΔcwlE）]在营养液培养基中培养24 h，将sigK突变菌株Bt-59 [Bt-59 （ΔsigK）]和cwlE和sigK双突变菌株Bt-59 [Bt-59 （ΔcwlE-sigK）]在营养液培养基中培养48 h。然后将5 μL的培养基转移到玻璃侧面，在光学显微镜下观察细胞形态和母细胞裂解情况。测定Bti菌株培养基在不同培养时间点的光密度，绘制Bt-59、Bt-59 （ΔcwlE）、Bt-59 （ΔsigK）和Bt-59 （ΔcwlE-sigK）菌株的生长曲线。采用Biolog微孔平板培养法分析了4株Bti菌株碳源代谢的差异，并用平均孔色发育（AWCD）估算了这些菌株的代谢活性。将4株Bti菌株的发酵培养基稀释至最终浓度为2.000、1.000、0.500、0.250和0.125 μL/L，测定4株菌株对Cx 3龄幼虫的中位致死浓度（LC50值）。测定淡色库蚊。另外，对Bti菌株的发酵培养基进行了如下处理：将pH调至5、7、9；在30、40、50℃下处理12小时；分别用紫外光照射0小时和6小时。然后选取20只三龄幼虫。将发酵后的终浓度为1 μL/L的淡色库蚊置于200 mL水中，26℃发酵24 h，测定其死亡率，评价pH、温度和紫外线照射对4种Bti菌株杀灭幼虫活性的影响。结果：Bt-59菌株及其突变体的生长曲线变化趋势相似，培养后6 h均进入稳定阶段。在Bt-59和Bt-59 （ΔcwlE）细胞中均发现孢子和晶体蛋白，而在Bt-59 （ΔsigK）和Bt-59 （ΔcwlE-sigK）细胞中仅发现晶体蛋白。Bt-59 （ΔcwlE-sigK）菌株细胞壁未见裂解，晶体蛋白嵌入母细胞。生物微孔板培养结果表明，4株Bti菌株的AWCD值随时间的变化趋势相似，4株菌株均代谢了33种碳源，包括糊精、d -麦芽糖和d -海藻糖。菌株Bt-59、Bt-59 （ΔcwlE）、Bt-59 （ΔsigK）和Bt-59 （ΔcwlE-sigK）发酵培养基对Cx的LC50值分别为0.60、0.51、0.70和0.72 μL/L。分别是淡色库蚊。校正后的幼虫死亡率。菌株Bt-59、Bt-59 （ΔcwlE）、Bt-59 （ΔsigK）和Bt-59 （ΔcwlE-sigK）的发酵培养基在pH = 5时相对于pH = 7时分别降低76.60%、76.00%、66.67%和0，且6 h的调整死亡率相对于0 h分别降低49.02%、51.06%、36.36%和4.44%。4株Bti发酵培养基在不同温度下处理后的校正死亡率为68.33% ~ 83.33%。结论：Bt-59 （ΔcwlE-sigK）菌株不产生孢子，不含cwlE和sigK不影响Bti菌株的生长、碳源代谢和对Cx幼虫的杀虫活性。侵害pallens。Bt-59 （ΔcwlE-sigK）菌株的细胞壁包埋可以保护Bti菌株的杀幼虫晶体蛋白免受外界环境因素的影响，包括紫外线照射和pH值的改变。

{"title":"[Physiological characteristics of Bacillus thuringiensis subspecies israelensis with double mutations of cwlE and sigK and its activity against larvae of Culex pipiens pallens].","authors":"L Huang, G Han, C Li, M Lin, N Zhang, J Xu","doi":"10.16250/j.32.1915.2024189","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024189","url":null,"abstract":"Objective: To investigate the physiological characteristics of Bacillus thuringiensis subspecies israelensis (Bti) with double mutations of cwlE and sigK genes and to assess the activity of Bti against larvae of Culex pipiens pallens under different external factors, so as to provide the theoretical evidence for the use of engineered bacteria of Bti for effective mosquito control.Methods: B. thuringiensis wild-type strain Bt-59 and Bt-59 strain with cwlE mutation [Bt-59 (ΔcwlE)] were cultured in nutrient broth media for 24 hours, and Bt-59 strains with sigK mutation [Bt-59 (ΔsigK)] and double mutations of cwlE and sigK [Bt-59 (ΔcwlE-sigK)] were cultured in nutrient broth media for 48 hours. Then, 5 μL of culture media were transferred to glass sides, and cell morphology and mother cell lysis were observed under an optical microscope. The optical densities of Bti strain culture media were measured at different time points of culture, and the growth curves of Bt-59, Bt-59 (ΔcwlE), Bt-59 (ΔsigK), and Bt-59 (ΔcwlE-sigK) strains were plotted. The differences in carbon source metabolism of four Bti strains were analyzed using the Biolog microplate culture method, and the metabolic activity of these strains was estimated with average well color development (AWCD). The fermentation media of these four Bti strains were diluted into final concentrations of 2.000, 1.000, 0.500, 0.250, and 0.125 μL/L, and the median lethal concentrations (LC50 values) of these four strains against the third instar larvae of Cx. pipiens pallens were determined. In addition, the fermentation media of Bti strains were processed as follows: pH adjusted to 5, 7 and 9; treated at 30, 40 ℃ and 50 ℃ for 12 hours; and exposed to irradiation with ultraviolet lights for 0 hour and 6 hours. Then, 20 third instar larvae of Cx. pipiens pallens were exposed to the above processed fermentation media at a final concentration of 1 μL/L in 200 mL of water at 26 ℃ for 24 hours, and the mosquito mortality was estimated to evaluate the effects of pH, temperature and ultraviolet irradiation on the larvicidal activity of four Bti strains.Results: The growth curves of the Bt-59 strain and its mutants shared a similar changing trend, and both experienced a stable phase 6 hours post-culture. Both spores and crystal proteins were found in Bt-59 and Bt-59 (ΔcwlE) cells, and only crystal proteins were found in Bt-59 (ΔsigK) and Bt-59 (ΔcwlE-sigK) cells. No lysis was found in the cell wall of the Bt-59 (ΔcwlE-sigK) strain, and the crystal protein was embedded in the mother cell. Biolog microplate culture assay showed that the AWCD values of four Bti strains showed a similar changing trend over time, and 33 carbon sources were found to be metabolized by all of the four strains,","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"606-613"},"PeriodicalIF":0.0,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Progress of researches on antiparasitic activities of scorpion venoms and their antimicrobial peptides]. [蝎子毒液及其抗菌肽抗寄生虫活性的研究进展]。

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-12-09 DOI: 10.16250/j.32.1915.2024139

Scorpion venom is a highly complicated cocktail of bioactive components including mucoproteins, enzymes, lipids, bioactive peptides, and other organic or inorganic molecules. Scorpion venom antimicrobial peptides are a class of small-molecule bioactive peptides extracted from scorpion venoms, which have shown a variety of biological activities, including antiviral, antibacterial, antifungal and antitumor actions. This review describes the progress of researches on the antiparasitic activities of scorpion venoms and their antimicrobial peptides, so as to provide insights into the research and development of novel antiparasitic agents.

蝎子毒液是一种高度复杂的生物活性成分混合物，包括黏液蛋白、酶、脂类、生物活性肽和其他有机或无机分子。蝎毒抗菌肽是从蝎毒中提取的一类小分子生物活性肽，具有抗病毒、抗菌、抗真菌和抗肿瘤等多种生物活性。本文综述了蝎毒液及其抗菌肽抗寄生虫活性的研究进展，以期为新型抗寄生虫药物的研究和开发提供参考。

引用次数: 0

[Health education and health promotion during the progress towards schistosomiasis elimination Ⅰ Investigation on health education needs and available resource based on integration of community resources]. [在消除血吸虫病进程中的健康教育和健康促进Ⅰ基于社区资源整合的健康教育需求和可用资源调查]。

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-11-29 DOI: 10.16250/j.32.1915.2024117

J Zhang, L Li, C Cao, S Xie, J Chen, Y Liu, H Xie, F Hu

Objective: To investigate the health education needs and available resources in schistosomiasis-endemic areas based on integration of community resources, and to explore the operation pathways for health education and promotion during the stage of schistosomiasis elimination.Methods: A community was selected from Nanji Township, Xinjian District, Nanchang City, Jiangxi Province around the Poyang Lake areas as the study site, and a questionnaire survey on health education needs for schistosomiasis control was conducted among permanent residents at ages of 20 years in the community during the period between June and July, 2022 using face-to-face interviews. In addition, the resources available for the community-based schistosomiasis control health education were investigated among workers in township-level specialized institutions, members of civil society organizations, villagers, teachers and high and primary school students through field observations, field surveys and thematic interviews.Results: A total of 304 individuals participated in the questionnaire survey on community-based health education needs for schistosomiasis control, and 94.41% (287/304) and 93.42% (284/304) of the respondents were most interested in knowing/understanding knowledge of the morphology of schistosomes and knowledge associated with the transmission route of schistosomiasis. The four most popular patterns for disseminating schistosomiasis control knowledge included live broadcasting of schistosomiasis control videos (94.41%, 287/304), printed brochures (89.80%, 273/304), special lectures by doctors (62.50%, 190/304) and physical health education materials for schistosomiasis control (61.51%, 187/304). There were 96.71% (294/304) and 77.63% (236/304) of respondents that accepted the participation of township healthcare center and township government/village committees in dissemination of schistosomiasis control knowledge, and there were 95.72% (291/304) and 80.59% (245/304) of respondents that mainly acquired schistosomiasis control knowledge through village allocation of leaflets and volunteer schistosomiasis control activities, respectively. There was 96.71% (294/304) of respondents participating in the dissemination activities of schistosomiasis control knowledge held by village committees, township healthcare centers, and high and primary schools/kinder-gartens, and 61.25% (177/289) of respondents were willing to participate in volunteer schistosomiasis control activities. A total of 115 individuals participated in the survey of available resources for community-based schistosomiasis control health education, and there were a large number of organizational, human and cultural resources in the community that were useful for health communication services.Conclusions: The abundant human and material resources from rural communities in schistosomiasis-endemic areas may be integ

目的：在整合社区资源的基础上，了解血吸虫病流行地区健康教育需求及现有资源，探索血吸虫病消灭阶段健康教育推广的操作途径。方法：选取江西省南昌市新建区南集乡鄱阳湖周边社区为研究点，于2022年6 - 7月采用面对面访谈法对该社区20岁常住居民进行血吸虫病防治健康教育需求问卷调查。此外，通过实地观察、实地调查和专题访谈，在乡镇一级专门机构的工作人员、民间社会组织成员、村民、教师和中学生中调查了社区血吸虫病控制健康教育的可用资源。结果：共有304人参与社区血吸虫病控制健康教育需求问卷调查，94.41%（287/304）和93.42%（284/304）的调查对象对血吸虫形态知识和血吸虫病传播途径相关知识的了解和了解最为感兴趣。传播血吸虫病防治知识的四种最常见的方式分别是直播血吸虫病防治视频（94.41%,283 /304）、印刷宣传册（89.80%,273/304）、医生专题讲座（62.50%,190/304）和血吸虫病防治体育健康教材（61.51%,187/304）。96.71%（294/304）和77.63%（236/304）的调查对象接受乡镇卫生院和乡政府/村委会参与血吸虫病防治知识的传播，95.72%（291/304）和80.59%（245/304）的调查对象主要通过村发传单和志愿血吸虫病防治活动获取血吸虫病防治知识。96.71%（294/304）的调查对象参加了村委会、乡镇卫生院、中小学/幼儿园举办的血吸虫病防治知识传播活动，61.25%（177/289）的调查对象表示愿意参加血吸虫病防治志愿者活动。社区血吸虫病防治健康教育现有资源调查共有115人参与，社区有大量的组织、人力和文化资源可用于卫生传播服务。结论：将血吸虫病流行地区农村社区丰富的人力物力资源整合到血吸虫病控制健康教育中，形成血吸虫病控制机构、乡镇政府、社区部门和社区居民共同参与的农村健康教育路径，为血吸虫病控制健康教育的未来发展提供参考。

{"title":"[Health education and health promotion during the progress towards schistosomiasis elimination Ⅰ Investigation on health education needs and available resource based on integration of community resources].","authors":"J Zhang, L Li, C Cao, S Xie, J Chen, Y Liu, H Xie, F Hu","doi":"10.16250/j.32.1915.2024117","DOIUrl":"https://doi.org/10.16250/j.32.1915.2024117","url":null,"abstract":"Objective: To investigate the health education needs and available resources in schistosomiasis-endemic areas based on integration of community resources, and to explore the operation pathways for health education and promotion during the stage of schistosomiasis elimination.Methods: A community was selected from Nanji Township, Xinjian District, Nanchang City, Jiangxi Province around the Poyang Lake areas as the study site, and a questionnaire survey on health education needs for schistosomiasis control was conducted among permanent residents at ages of 20 years in the community during the period between June and July, 2022 using face-to-face interviews. In addition, the resources available for the community-based schistosomiasis control health education were investigated among workers in township-level specialized institutions, members of civil society organizations, villagers, teachers and high and primary school students through field observations, field surveys and thematic interviews.Results: A total of 304 individuals participated in the questionnaire survey on community-based health education needs for schistosomiasis control, and 94.41% (287/304) and 93.42% (284/304) of the respondents were most interested in knowing/understanding knowledge of the morphology of schistosomes and knowledge associated with the transmission route of schistosomiasis. The four most popular patterns for disseminating schistosomiasis control knowledge included live broadcasting of schistosomiasis control videos (94.41%, 287/304), printed brochures (89.80%, 273/304), special lectures by doctors (62.50%, 190/304) and physical health education materials for schistosomiasis control (61.51%, 187/304). There were 96.71% (294/304) and 77.63% (236/304) of respondents that accepted the participation of township healthcare center and township government/village committees in dissemination of schistosomiasis control knowledge, and there were 95.72% (291/304) and 80.59% (245/304) of respondents that mainly acquired schistosomiasis control knowledge through village allocation of leaflets and volunteer schistosomiasis control activities, respectively. There was 96.71% (294/304) of respondents participating in the dissemination activities of schistosomiasis control knowledge held by village committees, township healthcare centers, and high and primary schools/kinder-gartens, and 61.25% (177/289) of respondents were willing to participate in volunteer schistosomiasis control activities. A total of 115 individuals participated in the survey of available resources for community-based schistosomiasis control health education, and there were a large number of organizational, human and cultural resources in the community that were useful for health communication services.Conclusions: The abundant human and material resources from rural communities in schistosomiasis-endemic areas may be integ","PeriodicalId":38874,"journal":{"name":"中国血吸虫病防治杂志","volume":"36 6","pages":"626-630"},"PeriodicalIF":0.0,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143013240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Plasma exchange combined with continuous renal replacement therapy for imported severe Plasmodium falciparum malaria: a case report]. 血浆置换联合持续肾替代治疗输入性重症恶性疟原虫疟疾1例

Q3 Medicine

中国血吸虫病防治杂志

Pub Date : 2024-11-15 DOI: 10.16250/j.32.1915.2024130

X Ma, B Li, X Yu, L Song, L Cheng

The article presents the diagnosis and treatment of an imported case with severe Plasmodium falciparum malaria, and the effect of plasma exchange combined with continuous renal replacement therapy. Severe P. falciparum malaria is characterized by complex clinical symptoms and multiple complications, and plasma exchange combined with continuous renal replacement therapy has a satisfactory therapeutic efficacy for severe P. falciparum malaria.

本文报道1例输入性重症恶性疟原虫疟疾的诊治及血浆置换联合持续肾替代治疗的效果。重症恶性疟原虫疟疾临床症状复杂，并发症多，血浆置换联合持续肾替代治疗对重症恶性疟原虫疟疾疗效满意。

引用次数: 0

中国血吸虫病防治杂志最新文献