一种多功能海洋铁氧化化学自养型生物的蛋白质组学比较

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-06-11 DOI:10.1111/1462-2920.16632
Roman A. Barco, N. Merino, B. Lam, B. Budnik, M. Kaplan, F. Wu, J. P. Amend, K. H. Nealson, D. Emerson
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摘要

本研究进行了比较蛋白质组分析,以确定海洋细菌 Ghiorsea bivora 中化学溶解自养铁氧化生物学功能的潜在遗传标记。迄今为止,这是Zetaproteobacteria类细菌中唯一非强制性铁氧化的特征物种,为研究蛋白质表达差异提供了一个独特的机会,以确定在中性pH下参与铁氧化的关键基因。在铁氧化和氢氧化条件下,共鉴定出 1000 多种蛋白质,并在两种处理中发现了不同表达的蛋白质。值得注意的是,发现了一个在铁氧化过程中上调的基因簇。该基因簇包含的编码细胞色素的基因与已知的铁氧化酶 Cyc2 具有序列相似性。有趣的是,这些在细菌和古细菌中都保留下来的细胞色素并没有表现出 Cyc2 典型的 β 管状结构。该细胞色素团可能编码一种生物纳米线状跨膜复合体,其中包含多种氧化还原蛋白,横跨内膜、周质、外膜和细胞外空间。反转录-PCR定量分析证实,在铁氧化条件下,与该复合物相关的关键基因上调。电微生物学方法进一步证实了这些发现,该方法证明了在阴极电位的三电极系统中 G. bivora 产生的负电流。这项研究为化学自养铁氧化的生物功能提供了重要见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

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Comparative proteomics of a versatile, marine, iron-oxidizing chemolithoautotroph

This study conducted a comparative proteomic analysis to identify potential genetic markers for the biological function of chemolithoautotrophic iron oxidation in the marine bacterium Ghiorsea bivora. To date, this is the only characterized species in the class Zetaproteobacteria that is not an obligate iron-oxidizer, providing a unique opportunity to investigate differential protein expression to identify key genes involved in iron-oxidation at circumneutral pH. Over 1000 proteins were identified under both iron- and hydrogen-oxidizing conditions, with differentially expressed proteins found in both treatments. Notably, a gene cluster upregulated during iron oxidation was identified. This cluster contains genes encoding for cytochromes that share sequence similarity with the known iron-oxidase, Cyc2. Interestingly, these cytochromes, conserved in both Bacteria and Archaea, do not exhibit the typical β-barrel structure of Cyc2. This cluster potentially encodes a biological nanowire-like transmembrane complex containing multiple redox proteins spanning the inner membrane, periplasm, outer membrane, and extracellular space. The upregulation of key genes associated with this complex during iron-oxidizing conditions was confirmed by quantitative reverse transcription-PCR. These findings were further supported by electromicrobiological methods, which demonstrated negative current production by G. bivora in a three-electrode system poised at a cathodic potential. This research provides significant insights into the biological function of chemolithoautotrophic iron oxidation.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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