{"title":"点头素对雌性斑马鱼(Danio rerio)生殖内分泌系统的毒理学影响。","authors":"Chayan Biswas, Madhuchhanda Adhikari, Kousik Pramanick","doi":"10.1016/j.aquatox.2024.107000","DOIUrl":null,"url":null,"abstract":"<div><p>Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (<em>Danio rerio</em>). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17β-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (<em>cyp19a1a</em>) in the ovary and primary vitellogenin genes (<em>vtg1, vtg2,</em> and <em>vtg3)</em> in the liver were down-regulated and marker genes for oxidative stress (<em>sod, cat,</em> and <em>gpx</em>) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.</p></div>","PeriodicalId":248,"journal":{"name":"Aquatic Toxicology","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Toxicological effects of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio)\",\"authors\":\"Chayan Biswas, Madhuchhanda Adhikari, Kousik Pramanick\",\"doi\":\"10.1016/j.aquatox.2024.107000\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (<em>Danio rerio</em>). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17β-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (<em>cyp19a1a</em>) in the ovary and primary vitellogenin genes (<em>vtg1, vtg2,</em> and <em>vtg3)</em> in the liver were down-regulated and marker genes for oxidative stress (<em>sod, cat,</em> and <em>gpx</em>) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.</p></div>\",\"PeriodicalId\":248,\"journal\":{\"name\":\"Aquatic Toxicology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-06-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Aquatic Toxicology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0166445X2400170X\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MARINE & FRESHWATER BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquatic Toxicology","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0166445X2400170X","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
Toxicological effects of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio)
Nodularin is a potent cyanotoxin that has been detected in aquatic environments as well as in the body of aquatic organisms throughout the world, but its effects on the reproductive system are yet to be explored. The present study investigated the toxic effects of environmentally relevant concentrations of nodularin on the reproductive endocrine system of female zebrafish (Danio rerio). After exposure to nodularin for 14 days, decreased gonadosomatic Index (GSI), germinal vesicle breakdown (GVBD), and decreased level of follicle-stimulating hormone (FSH), luteinizing hormone (LH), 17β-estradiol (E2) level and increased testosterone (T) content in female zebrafish suggested that nodularin may disrupt both oocyte growth and maturation. In support of this data, alteration in different marker gene expression on the hypothalamic-pituitary-gonadal-liver (HPGL) axis was observed. Transcriptional levels of genes related to steroidogenesis including cytochrome P450 aromatase (cyp19a1a) in the ovary and primary vitellogenin genes (vtg1, vtg2, and vtg3) in the liver were down-regulated and marker genes for oxidative stress (sod, cat, and gpx) were up-regulated on HPGL axis. These findings revealed for the first time that nodularin is a potent endocrine-disrupting compound posing oxidative stress and causes reproductive endocrine toxicity in female zebrafish, emphasizing the importance of assessing its environmental risks.
期刊介绍:
Aquatic Toxicology publishes significant contributions that increase the understanding of the impact of harmful substances (including natural and synthetic chemicals) on aquatic organisms and ecosystems.
Aquatic Toxicology considers both laboratory and field studies with a focus on marine/ freshwater environments. We strive to attract high quality original scientific papers, critical reviews and expert opinion papers in the following areas: Effects of harmful substances on molecular, cellular, sub-organismal, organismal, population, community, and ecosystem level; Toxic Mechanisms; Genetic disturbances, transgenerational effects, behavioral and adaptive responses; Impacts of harmful substances on structure, function of and services provided by aquatic ecosystems; Mixture toxicity assessment; Statistical approaches to predict exposure to and hazards of contaminants
The journal also considers manuscripts in other areas, such as the development of innovative concepts, approaches, and methodologies, which promote the wider application of toxicological datasets to the protection of aquatic environments and inform ecological risk assessments and decision making by relevant authorities.