球茶藨子 CGMCC 6882 产生的多糖对受 H2O2 挑战的 HepG2 细胞的抗氧化保护作用

Zichao Wang , Yi Zheng , Wenzhuo Lu , Jinchu Yang , Yingjie Feng , Zhitao Li , Na Li , Yahui Yang , Qi Wang , Lemei An
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引用次数: 0

摘要

有效利用厨房废油(WKO)对环境保护和经济发展具有重要意义。目前,一种内生真菌 Chaetomium globosum CGMCC 6882 多糖(CGP-WKO)在以 WKO 为唯一碳源的浸没式发酵中被成功生产出来。CGP-WKO 的产量为 1.53 ± 0.27 克/升,碳水化合物含量为 95.85 % ± 3.02 %,蛋白质含量为 2.94 % ± 0.62 %。结构特征分析表明,CGP-WKO 含有葡萄糖、氨基葡萄糖、甘露糖、鼠李糖、半乳糖、果糖和葡萄糖醛酸,摩尔比为 30.38:1.34:32.22:9.68:1.59:0.62:3.73。CGP-WKO 的重量平均分子量为 26.64 kDa,多分散性为 1.48。此外,体外抗氧化能力检测表明,当 CGP-WKO 的浓度为 1.0 毫克/毫升时,其对 1,1-二苯基-2-苦基肼、2,2′-偶氮二-二(3-乙基-苯并噻唑啉-6-磺酸)二铵盐、羟自由基和超氧自由基的清除率分别为 45.89 % ± 1.89 %、55.89 % ± 1.12 %、29.02 % ± 2.61 % 和 52.76 % ± 2.12 %;IC50 值分别为 6.4612、0.8888、13.8585 和 0.9398 mg/mL。CGP-WKO 增加了 H2O2 挑战的 HepG2 细胞中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的含量,降低了丙二醛的含量。总之,本研究表明 CGP-WKO 可作为一种抗氧化剂用于食品、化妆品和制药行业。
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Antioxidant protection of a polysaccharide produced by Chaetomium globosum CGMCC 6882 on H2O2-challenged HepG2 cells

Effective utilization of waste kitchen oil (WKO) is important to environmental protection and economic development. Presently, an endophytic fungus Chaetomium globosum CGMCC 6882 polysaccharide (CGP-WKO) was successfully produced through submerged fermentation with the sole carbon source of WKO. CGP-WKO had a yield of 1.53 ± 0.27 g/L and contained 95.85 % ± 3.02 % carbohydrate and 2.94 % ± 0.62 % protein. Structural feature analysis indicated that CGP-WKO contained glucose, glucosamine, mannose, rhamnose, galactose, fructose, and glucuronic acid in a molar ratio of 30.38: 1.34: 32.22: 9.68: 1.59: 0.62: 3.73. The weight-averaged molecular weight of CGP-WKO was 26.64 kDa, and its polydispersity was 1.48. Moreover, antioxidant capacity detection in vitro demonstrated that when the concentration of CGP-WKO was 1.0 mg/mL, its scavenging effects against 1,1-diphenyl-2-picrylhydrazyl; 2,2′-azinobis-di-(3-ethyl-benzothiazolin-6-sulfonic acid) diammonium salt; and hydroxyl and superoxide radicals were 45.89 % ± 1.89 %, 55.89 % ± 1.12 %, 29.02 % ± 2.61 %, and 52.76 % ± 2.12 %, respectively; and the IC50 values were 6.4612, 0.8888, 13.8585, and 0.9398 mg/mL, respectively. CGP-WKO increased the content of superoxide dismutase, catalase, and glutathione peroxidase and reduced malondialdehyde content in H2O2-challenged HepG2 cells. Overall, the present work indicated that CGP-WKO can be used as an antioxidant agent in the food, cosmetics, and pharmaceutical industries.

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