Carbohydrate Polymer Technologies and Applications最新文献

Carboxymethyl chitosan oligosaccharide prevents the progression of chronic kidney disease as a Nrf2-dependent apoptosis inhibitor

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100728

Xiaozheng Yuan , Huan He , Youtao Xu, Xinru Chen, Jiawen Wu, Xianpeng Zhong, Xiyu Li, Jing Qiao

Chronic Kidney Disease (CKD) has become a global public health problem, for which satisfactory treatments remain an urgent need. Carboxymethyl chitosan oligosaccharide (CMCOS) is a small molecule derivative of the natural product chitin, which is easy to absorb with variety of biological activities. Our previous research has demonstrated the renal protective effects of CMCOS, while the underlying mechanism is poorly understood. This study reports that the renal protective effects of CMCOS are associated with anti-apoptosis in glomerular podocytes and renal tubular epithelial cells, which maintains the integrity of renal structure and function. Furthermore, CMCOS suppresses tissue oxidative stress and intracellular accumulation of reactive oxygen species both in vitro and in vivo, demonstrating positive correlations with its apoptosis inhibition. Mechanistically, CMCOS enhances endogenous antioxidant defense and relieves oxidative stress in the renal microenvironment through activation of the nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. Specific inhibition of Nrf2 abolishes the apoptosis-inhibitory effect of CMCOS, indicating a dependence on this pathway. In conclusion, CMCOS activates Nrf2/HO-1 signaling pathway to inhibited oxidative stress-related apoptosis in renal parenchymal cells, thereby alleviating the progression of CKD. Our findings provide a new basis for the medicinal development of carbohydrates.

{"title":"Carboxymethyl chitosan oligosaccharide prevents the progression of chronic kidney disease as a Nrf2-dependent apoptosis inhibitor","authors":"Xiaozheng Yuan , Huan He , Youtao Xu, Xinru Chen, Jiawen Wu, Xianpeng Zhong, Xiyu Li, Jing Qiao","doi":"10.1016/j.carpta.2025.100728","DOIUrl":"10.1016/j.carpta.2025.100728","url":null,"abstract":"<div><div>Chronic Kidney Disease (CKD) has become a global public health problem, for which satisfactory treatments remain an urgent need. Carboxymethyl chitosan oligosaccharide (CMCOS) is a small molecule derivative of the natural product chitin, which is easy to absorb with variety of biological activities. Our previous research has demonstrated the renal protective effects of CMCOS, while the underlying mechanism is poorly understood. This study reports that the renal protective effects of CMCOS are associated with anti-apoptosis in glomerular podocytes and renal tubular epithelial cells, which maintains the integrity of renal structure and function. Furthermore, CMCOS suppresses tissue oxidative stress and intracellular accumulation of reactive oxygen species both in vitro and in vivo, demonstrating positive correlations with its apoptosis inhibition. Mechanistically, CMCOS enhances endogenous antioxidant defense and relieves oxidative stress in the renal microenvironment through activation of the nuclear factor-erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway. Specific inhibition of Nrf2 abolishes the apoptosis-inhibitory effect of CMCOS, indicating a dependence on this pathway. In conclusion, CMCOS activates Nrf2/HO-1 signaling pathway to inhibited oxidative stress-related apoptosis in renal parenchymal cells, thereby alleviating the progression of CKD. Our findings provide a new basis for the medicinal development of carbohydrates.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100728"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailored biopolymer films based on cellulose acetate and cobalt ferrite nanoparticles: Dye adsorption and antimicrobial activity

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100716

Mohamed S. Hasanin , Miroslawa El Fray , O.A. Al-Qabandi , Mohamed Bassyouni , N. Almutlaq , Qihui Zhou , Tomy Muringayil Joseph , Monica Toderaș , Sherief A. Al Kiey

In this work, easy-collect adsorbent strips were developed based on the inclusion of cellulose acetate and poly(ԑ-caprolactone) (CA/PCL) as a template for cobalt ferrite nanoparticles (CoFe₂O₄NPs) with different concentrations (1, 3, and 5 %). The formulated strips and their parent components were characterized using FTIR and XRD. In addition, topographical analysis, including TEM for CoFe₂O₄NPs and SEM and EDX, was performed for formulated strips. The kinetics and the isotherm models were applied to explain the adsorption mechanism. The antimicrobial activity of the formulated strips was studied against the popular water-contaminated microorganism strains. The antimicrobial activity of the strips was presented as a broad spectrum antibacterial activity, and antifungal activity against unicellular and filaments was increased in parallel with CoFe₂O₄NPs concentration. The kinetics and isotherm models emphasized that the cationic dye was fitted with a pseudo-first-order and that the anionic dye was fitted with a pseudo-second-order. Moreover, the Langmuir and Freundlich isotherm models were fitted to cationic and anionic dyes. CA/PCL@CoFe₂O₄NPs (5 %) stripe recorded q_m as 1567 and 908 mg g^-1 for methylene blue (MB) and methyl orange (MO), respectively. These findings recommended these stripes for multi-dye adsorption.

{"title":"Tailored biopolymer films based on cellulose acetate and cobalt ferrite nanoparticles: Dye adsorption and antimicrobial activity","authors":"Mohamed S. Hasanin , Miroslawa El Fray , O.A. Al-Qabandi , Mohamed Bassyouni , N. Almutlaq , Qihui Zhou , Tomy Muringayil Joseph , Monica Toderaș , Sherief A. Al Kiey","doi":"10.1016/j.carpta.2025.100716","DOIUrl":"10.1016/j.carpta.2025.100716","url":null,"abstract":"<div><div>In this work, easy-collect adsorbent strips were developed based on the inclusion of cellulose acetate and poly(ԑ-caprolactone) (CA/PCL) as a template for cobalt ferrite nanoparticles (CoFe<sub>2</sub>O<sub>4</sub>NPs) with different concentrations (1, 3, and 5 %). The formulated strips and their parent components were characterized using FTIR and XRD. In addition, topographical analysis, including TEM for CoFe<sub>2</sub>O<sub>4</sub>NPs and SEM and EDX, was performed for formulated strips. The kinetics and the isotherm models were applied to explain the adsorption mechanism. The antimicrobial activity of the formulated strips was studied against the popular water-contaminated microorganism strains. The antimicrobial activity of the strips was presented as a broad spectrum antibacterial activity, and antifungal activity against unicellular and filaments was increased in parallel with CoFe<sub>2</sub>O<sub>4</sub>NPs concentration. The kinetics and isotherm models emphasized that the cationic dye was fitted with a pseudo-first-order and that the anionic dye was fitted with a pseudo-second-order. Moreover, the Langmuir and Freundlich isotherm models were fitted to cationic and anionic dyes. CA/PCL@CoFe<sub>2</sub>O<sub>4</sub>NPs (5 %) stripe recorded q<sub>m</sub> as 1567 and 908 mg g<sup>-1</sup> for methylene blue (MB) and methyl orange (MO), respectively. These findings recommended these stripes for multi-dye adsorption.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100716"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143509559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellulose oligomer synthesis: Primer effects on structural characteristics in the cellodextrin phosphorylase-catalyzed reverse reaction 纤维素低聚物的合成：引物对纤维糊精磷酸化酶催化的反向反应结构特征的影响

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100731

Tatsuhiro Konishi , Atsushi Sasaki , Ryosuke Kusumi , Masahisa Wada , Kayoko Kobayashi

The synthesis of cellulose oligomers catalyzed by cellodextrin phosphorylase (CDP) can easily yield microcrystals with functional groups exposed on their surfaces using glucose derivatives with various functional groups at the C1 position as primers. In this study, we examined and compared the effects of primers with various structures on the structural characteristics of the resulting synthetic products. The yield of the synthetic products positively correlated with the CDP activity toward the primer, whereas the degree of polymerization (DP) and molecular weight distribution showed an inverse correlation. However, deviations from these trends were observed, suggesting the influence of varying solubilities and crystallization behaviors due to the introduction of functional groups. The obtained microcrystals exhibited a primarily plate-like shape with a thickness increasing in proportion to the DP, resulting in larger plate-like crystals at higher DP values. These findings provide insights into strategies for controlling the structure of the resulting cellulose oligomers.

{"title":"Cellulose oligomer synthesis: Primer effects on structural characteristics in the cellodextrin phosphorylase-catalyzed reverse reaction","authors":"Tatsuhiro Konishi , Atsushi Sasaki , Ryosuke Kusumi , Masahisa Wada , Kayoko Kobayashi","doi":"10.1016/j.carpta.2025.100731","DOIUrl":"10.1016/j.carpta.2025.100731","url":null,"abstract":"<div><div>The synthesis of cellulose oligomers catalyzed by cellodextrin phosphorylase (CDP) can easily yield microcrystals with functional groups exposed on their surfaces using glucose derivatives with various functional groups at the C1 position as primers. In this study, we examined and compared the effects of primers with various structures on the structural characteristics of the resulting synthetic products. The yield of the synthetic products positively correlated with the CDP activity toward the primer, whereas the degree of polymerization (DP) and molecular weight distribution showed an inverse correlation. However, deviations from these trends were observed, suggesting the influence of varying solubilities and crystallization behaviors due to the introduction of functional groups. The obtained microcrystals exhibited a primarily plate-like shape with a thickness increasing in proportion to the DP, resulting in larger plate-like crystals at higher DP values. These findings provide insights into strategies for controlling the structure of the resulting cellulose oligomers.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100731"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143519321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amorphous calcium phosphate reinforced alginate-dialdehyde-gelatin (ADA-GEL) bioink for biofabrication of bone tissue scaffolds

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100710

Abhishek Indurkar , Susanne Heid , Julian Bauer , Kristaps Rubenis , Oliver Friedrich , Janis Locs , Aldo R. Boccaccini

The aim of this work was to develop a nanocomposite bioink closely resembling the nanostructure of bone incorporating amorphous calcium phosphate (ACP) as an inorganic counterpart, specifically, citrate stabilized ACP (ACP_CIT) and non-stabilized ACP (ACP_ACE) in an organic hydrogel matrix consisted of alginate dialdehyde-gelatin (ADA-GEL). The hydrogel's physical properties were evaluated, confirming the reinforcement effect of ACP. The frequency sweep analysis showed that G' and G" of ADA-GEL were 99 ± 9 Pa and 9 ± 1 Pa, respectively. By the addition of ACP_ACE, G' and G" increased. Overall, the viscoelastic and mechanical properties of ADA-GEL hydrogel were enhanced by ACP. ACP_CIT was more effective than ACP_ACE. Further, printing parameters were optimized. The bioink was formulated by embedding MC3T3-E1 cells in ADA-GEL and ACP-reinforced ADA-GEL hydrogels, followed by fabricating scaffolds at optimized printing parameters (pressure: 65 kPa, speed: 5 mm/s). Crosslinking was performed by immersing constructs in CaCl₂ and microbial transglutaminase solution. Post-printing analysis was performed using the printability index and average pore area analysis. The lowest structural stability was observed in ADA-GEL constructs. The highest structural stability was noted in ADA-GEL-ACP_CIT constructs. Epifluorescence and two-photon microscopy of Rhodamine/Phalloidin stained constructs confirmed the cytocompatibility of the bioinks.

{"title":"Amorphous calcium phosphate reinforced alginate-dialdehyde-gelatin (ADA-GEL) bioink for biofabrication of bone tissue scaffolds","authors":"Abhishek Indurkar , Susanne Heid , Julian Bauer , Kristaps Rubenis , Oliver Friedrich , Janis Locs , Aldo R. Boccaccini","doi":"10.1016/j.carpta.2025.100710","DOIUrl":"10.1016/j.carpta.2025.100710","url":null,"abstract":"<div><div>The aim of this work was to develop a nanocomposite bioink closely resembling the nanostructure of bone incorporating amorphous calcium phosphate (ACP) as an inorganic counterpart, specifically, citrate stabilized ACP (ACP_CIT) and non-stabilized ACP (ACP_ACE) in an organic hydrogel matrix consisted of alginate dialdehyde-gelatin (ADA-GEL). The hydrogel's physical properties were evaluated, confirming the reinforcement effect of ACP. The frequency sweep analysis showed that G' and G\" of ADA-GEL were 99 ± 9 Pa and 9 ± 1 Pa, respectively. By the addition of ACP_ACE, G' and G\" increased. Overall, the viscoelastic and mechanical properties of ADA-GEL hydrogel were enhanced by ACP. ACP_CIT was more effective than ACP_ACE. Further, printing parameters were optimized. The bioink was formulated by embedding MC3T3-E1 cells in ADA-GEL and ACP-reinforced ADA-GEL hydrogels, followed by fabricating scaffolds at optimized printing parameters (pressure: 65 kPa, speed: 5 mm/s). Crosslinking was performed by immersing constructs in CaCl<sub>2</sub> and microbial transglutaminase solution. Post-printing analysis was performed using the printability index and average pore area analysis. The lowest structural stability was observed in ADA-GEL constructs. The highest structural stability was noted in ADA-GEL-ACP_CIT constructs. Epifluorescence and two-photon microscopy of Rhodamine/Phalloidin stained constructs confirmed the cytocompatibility of the bioinks.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100710"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydroxyapatites and nano-hydroxyapatites as scaffolds in drug delivery towards efficient bone regeneration: A review

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100692

Amir Hatami kaleshtari , Samira Farjaminejad , Melika Hasani , Rosana Farjaminejad , Amin Foroozandeh , Majid Abdouss , Mohammad Hasanzadeh

Bone regeneration is a complex process that requires materials capable of supporting structural integrity, promoting cell proliferation, and enabling effective drug delivery. Nano-hydroxyapatite (nHA), with its biocompatibility and close resemblance to natural bone minerals, has emerged as a promising material in bone tissue engineering. nHA enhances osteoconductivity, mechanical strength, and drug-loading capacity, making it an effective tool for treating bone defects. Therefore, the potential of combining nHA with bioactive agents and polymers offers new opportunities for long-term, targeted treatments for bone diseases and injuries. This review highlights the role of nHA in drug-delivery systems for bone regeneration, focusing on its properties, synthesis methods, and interactions with bone tissue. Then, key challenges include optimizing scaffold porosity, controlling degradation rates, and improving mechanical stability. Next, advanced applications, such as gene therapy and intelligent drug delivery systems, are also explored. Additionally, some research gaps on the utilization of nHA in drug delivery of bone regeneration were surveyed. Finally, future perspectives on the nHA-based scaffolds for smart drug delivery in the reconstruction and repair of bone were discussed.

{"title":"Hydroxyapatites and nano-hydroxyapatites as scaffolds in drug delivery towards efficient bone regeneration: A review","authors":"Amir Hatami kaleshtari , Samira Farjaminejad , Melika Hasani , Rosana Farjaminejad , Amin Foroozandeh , Majid Abdouss , Mohammad Hasanzadeh","doi":"10.1016/j.carpta.2025.100692","DOIUrl":"10.1016/j.carpta.2025.100692","url":null,"abstract":"<div><div>Bone regeneration is a complex process that requires materials capable of supporting structural integrity, promoting cell proliferation, and enabling effective drug delivery. Nano-hydroxyapatite (nHA), with its biocompatibility and close resemblance to natural bone minerals, has emerged as a promising material in bone tissue engineering. nHA enhances osteoconductivity, mechanical strength, and drug-loading capacity, making it an effective tool for treating bone defects. Therefore, the potential of combining nHA with bioactive agents and polymers offers new opportunities for long-term, targeted treatments for bone diseases and injuries. This review highlights the role of nHA in drug-delivery systems for bone regeneration, focusing on its properties, synthesis methods, and interactions with bone tissue. Then, key challenges include optimizing scaffold porosity, controlling degradation rates, and improving mechanical stability. Next, advanced applications, such as gene therapy and intelligent drug delivery systems, are also explored. Additionally, some research gaps on the utilization of nHA in drug delivery of bone regeneration were surveyed. Finally, future perspectives on the nHA-based scaffolds for smart drug delivery in the reconstruction and repair of bone were discussed.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100692"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143529132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microwave assisted extraction of chitosan from Agaricus bisporus: techno-functional and microstructural properties 微波辅助提取双孢蘑菇壳聚糖：技术功能和微观结构特性

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-03-01 DOI: 10.1016/j.carpta.2025.100730

Adity Bahndral , Rafeeya Shams , Kshirod Kumar Dash , Pintu Chaudhary , Ayaz Mukarram Shaikh , Kovács Béla

Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is primarily derived from chitin. The present research was conducted to generate and analyze chitosan derived from white button mushroom waste (Agaricus bisporus) using microwave assisted extraction. Dried mushroom waste powder was demineralized in diluted acid using 3 M HCl in 1:10 w/v at 540 W for 8 min and deproteinated at 180 W using 10% NaOH in 1:10 w/v for 8 min to remove proteins and lipids. The extracted chitin was deacetylated using 50% NaOH in 1:20 w/v at 360 W to convert it into chitosan. Chitin from the aforesaid process was deacetylated in concentrated alkaline medium at 360 W for 8 min to yield chitosan by converting acetyl groups to -NH₂ groups. The pH and solubility of fresh chitosan were 7.5 and 75%, respectively. Extracted chitosan had maximum 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of 53.97% and reducing power of 3.58. The microwave irradiation method produced chitosan having degree of deacetylation of 79.94% and crystallinity index of 1.09. The spectra bands confirmed existence of NH₂, OH, CO, CH, and CN functional groups. The X-ray diffraction analysis of the chitosan sample discovered distinct peaks at 2θ values between 10 and 20 °, indicating its semi-crystalline nature.

{"title":"Microwave assisted extraction of chitosan from Agaricus bisporus: techno-functional and microstructural properties","authors":"Adity Bahndral , Rafeeya Shams , Kshirod Kumar Dash , Pintu Chaudhary , Ayaz Mukarram Shaikh , Kovács Béla","doi":"10.1016/j.carpta.2025.100730","DOIUrl":"10.1016/j.carpta.2025.100730","url":null,"abstract":"<div><div>Chitosan, a copolymer of glucosamine and N-acetyl glucosamine, is primarily derived from chitin. The present research was conducted to generate and analyze chitosan derived from white button mushroom waste (<em>Agaricus bisporus</em>) using microwave assisted extraction. Dried mushroom waste powder was demineralized in diluted acid using 3 M HCl in 1:10 w/v at 540 W for 8 min and deproteinated at 180 W using 10% NaOH in 1:10 w/v for 8 min to remove proteins and lipids. The extracted chitin was deacetylated using 50% NaOH in 1:20 w/v at 360 W to convert it into chitosan. Chitin from the aforesaid process was deacetylated in concentrated alkaline medium at 360 W for 8 min to yield chitosan by converting acetyl groups to -NH<sub>2</sub> groups. The pH and solubility of fresh chitosan were 7.5 and 75%, respectively. Extracted chitosan had maximum 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity of 53.97% and reducing power of 3.58. The microwave irradiation method produced chitosan having degree of deacetylation of 79.94% and crystallinity index of 1.09. The spectra bands confirmed existence of NH<sub>2</sub>, OH, C<img>O, CH, and C<img>N functional groups. The X-ray diffraction analysis of the chitosan sample discovered distinct peaks at 2θ values between 10 and 20 °, indicating its semi-crystalline nature.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100730"},"PeriodicalIF":6.2,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cyclodextrin metal-organic framework for efficient adsorption of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) residues from cultured meat

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-26 DOI: 10.1016/j.carpta.2025.100727

Tahirou Sogore , Meimei Guo , Na Sun , Donglei Jiang , Donghong Liu , Mofei Shen , Tian Ding

Cultured meat offers a promising solution to meet global protein demand using stem cells rather than traditional animal agriculture. The production relies on gel-based scaffold materials, requiring photoinitiators like lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for crosslinking and forming the three-dimensional matrix. However, LAP residues in the final product pose potential health risks, necessitating efficient detection and removal strategies. In this study, we analyzed cultured fish meat samples, revealing LAP residues, then prepared an eco-friendly CD-MOF from edible materials and demonstrated its excellent LAP adsorption capabilities. The adsorption quantity was investigated using high-performance liquid chromatography (HPLC), and the method's versatility was validated by expanding the study to 2-Hydroxy-4′-(2- hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959) and 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651). CD-MOF achieved maximum adsorption rates of 88.19 ± 3.65 mg/g for LAP, 60.88 ± 3.51 mg/g for Irgacure 2959, and 56.52 ± 3.28 mg/g for Irgacure 651. Spiked recoveries in cultured meat samples ranged from 84.36 % to 98.78 %, with RSDs between 2.16 % and 6.87 %, demonstrating high precision. These findings suggest CD-MOF's promise for detecting and removing photoinitiator residues in cultured meat production.

{"title":"Cyclodextrin metal-organic framework for efficient adsorption of lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) residues from cultured meat","authors":"Tahirou Sogore , Meimei Guo , Na Sun , Donglei Jiang , Donghong Liu , Mofei Shen , Tian Ding","doi":"10.1016/j.carpta.2025.100727","DOIUrl":"10.1016/j.carpta.2025.100727","url":null,"abstract":"<div><div>Cultured meat offers a promising solution to meet global protein demand using stem cells rather than traditional animal agriculture. The production relies on gel-based scaffold materials, requiring photoinitiators like lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) for crosslinking and forming the three-dimensional matrix. However, LAP residues in the final product pose potential health risks, necessitating efficient detection and removal strategies. In this study, we analyzed cultured fish meat samples, revealing LAP residues, then prepared an eco-friendly CD-MOF from edible materials and demonstrated its excellent LAP adsorption capabilities. The adsorption quantity was investigated using high-performance liquid chromatography (HPLC), and the method's versatility was validated by expanding the study to 2-Hydroxy-4′-(2- hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959) and 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651). CD-MOF achieved maximum adsorption rates of 88.19 ± 3.65 mg/g for LAP, 60.88 ± 3.51 mg/g for Irgacure 2959, and 56.52 ± 3.28 mg/g for Irgacure 651. Spiked recoveries in cultured meat samples ranged from 84.36 % to 98.78 %, with RSDs between 2.16 % and 6.87 %, demonstrating high precision. These findings suggest CD-MOF's promise for detecting and removing photoinitiator residues in cultured meat production.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100727"},"PeriodicalIF":6.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel (pectin), oleogel (oil/triglyceride) and bigel (alginate-oil/triglyceride) and their applications in meat products formulation

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-21 DOI: 10.1016/j.carpta.2025.100715

Farzad Mirab Toupchi, Sajad Pirsa, Sana Mohammadi

The consumption of meat and meat products holds significant importance and nutritional value. Given their formulation, these products can be deemed complete, as they encompass all essential food groups. While meat constitutes the majority of the formulation, other ingredients may pose health risks to consumers. This article aims to first analyze the key ingredients in meat product formulations and their impact on the final product. It also explores natural and suitable substitutes that maintain the characteristics of meat products without compromising quality.

The primary focus of this review is to describe and report on various types of gels—namely hydrogel, organogel, emulsion gel, and bigel—and their application in fat replacement within meat products. The article details the structure, production methods, and processing of these various edible gels. Additionally, it discusses the substitution of the harmful substance nitrite in meat products with natural preservatives, along with the replacement of NaCl and phosphate salts with less hazardous alternatives. Finally, the role of fermentation processes in meat products is investigated and reported.

{"title":"Hydrogel (pectin), oleogel (oil/triglyceride) and bigel (alginate-oil/triglyceride) and their applications in meat products formulation","authors":"Farzad Mirab Toupchi, Sajad Pirsa, Sana Mohammadi","doi":"10.1016/j.carpta.2025.100715","DOIUrl":"10.1016/j.carpta.2025.100715","url":null,"abstract":"<div><div>The consumption of meat and meat products holds significant importance and nutritional value. Given their formulation, these products can be deemed complete, as they encompass all essential food groups. While meat constitutes the majority of the formulation, other ingredients may pose health risks to consumers. This article aims to first analyze the key ingredients in meat product formulations and their impact on the final product. It also explores natural and suitable substitutes that maintain the characteristics of meat products without compromising quality.</div><div>The primary focus of this review is to describe and report on various types of gels—namely hydrogel, organogel, emulsion gel, and bigel—and their application in fat replacement within meat products. The article details the structure, production methods, and processing of these various edible gels. Additionally, it discusses the substitution of the harmful substance nitrite in meat products with natural preservatives, along with the replacement of NaCl and phosphate salts with less hazardous alternatives. Finally, the role of fermentation processes in meat products is investigated and reported.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100715"},"PeriodicalIF":6.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing handheld inkjet printing technology for rapid and decentralised fabrication of drug-loaded hydroxypropyl cellulose buccal films

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-20 DOI: 10.1016/j.carpta.2025.100724

Paola Carou-Senra , Atheer Awad , Abdul W. Basit , Carmen Alvarez-Lorenzo , Alvaro Goyanes

Inkjet printing is emerging as a valuable tool for personalised medicine, offering precision and flexibility in pharmaceutical development. Efforts have been made to modify commercial desktop printers; however, the growing interest in decentralized and on-demand pharmaceutical production highlights the need for more compact, energy-efficient alternatives that enhance versatility and seamless integration across diverse environments. This study presents for the first time, a handheld inkjet printer for the portable production of personalised medications in decentralised settings using hydroxypropyl cellulose (HPC) films as the printing substrate. The compact design of handheld printers ensures ease of use, space efficiency, and reduced energy consumption, making them ideal for patient-centric applications. As a proof of concept, the Selpic S1+ handheld inkjet printer was used to create buccal films with flexible nicotine doses tailored for nicotine replacement therapy. By modifying the printed area and number of pharma-ink (drug-loaded pharmaceutical ink) layers, precise nicotine dosages were achieved. The films exhibited controlled drug release, strong mucoadhesive properties, and adequate mechanical properties for buccal application. Studies on swelling behaviour, mucoadhesion, and surface morphology validated the film structural integrity and functionality. Ex vivo permeation studies using porcine buccal mucosa demonstrated high nicotine permeability. Additionally, pharma-ink printing was successfully applied to other biological macromolecules substrates (starch and cellulose), underscoring the versatility of handheld devices in creating diverse patterns across different surfaces. This research highlights the potential of handheld inkjet printing on eco-friendly films for decentralised, rapid and affordable treatment customisation with improved therapeutic outcomes and patient adherence.

{"title":"Harnessing handheld inkjet printing technology for rapid and decentralised fabrication of drug-loaded hydroxypropyl cellulose buccal films","authors":"Paola Carou-Senra , Atheer Awad , Abdul W. Basit , Carmen Alvarez-Lorenzo , Alvaro Goyanes","doi":"10.1016/j.carpta.2025.100724","DOIUrl":"10.1016/j.carpta.2025.100724","url":null,"abstract":"<div><div>Inkjet printing is emerging as a valuable tool for personalised medicine, offering precision and flexibility in pharmaceutical development. Efforts have been made to modify commercial desktop printers; however, the growing interest in decentralized and on-demand pharmaceutical production highlights the need for more compact, energy-efficient alternatives that enhance versatility and seamless integration across diverse environments. This study presents for the first time, a handheld inkjet printer for the portable production of personalised medications in decentralised settings using hydroxypropyl cellulose (HPC) films as the printing substrate. The compact design of handheld printers ensures ease of use, space efficiency, and reduced energy consumption, making them ideal for patient-centric applications. As a proof of concept, the Selpic S1+ handheld inkjet printer was used to create buccal films with flexible nicotine doses tailored for nicotine replacement therapy. By modifying the printed area and number of pharma-ink (drug-loaded pharmaceutical ink) layers, precise nicotine dosages were achieved. The films exhibited controlled drug release, strong mucoadhesive properties, and adequate mechanical properties for buccal application. Studies on swelling behaviour, mucoadhesion, and surface morphology validated the film structural integrity and functionality. <em>Ex vivo</em> permeation studies using porcine buccal mucosa demonstrated high nicotine permeability. Additionally, pharma-ink printing was successfully applied to other biological macromolecules substrates (starch and cellulose), underscoring the versatility of handheld devices in creating diverse patterns across different surfaces. This research highlights the potential of handheld inkjet printing on eco-friendly films for decentralised, rapid and affordable treatment customisation with improved therapeutic outcomes and patient adherence.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100724"},"PeriodicalIF":6.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of polyvinyl alcohol-gum Arabic hydrogel nanocomposite for xylanase immobilization and its role in poultry feed enhancement

IF 6.2 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymer Technologies and Applications

Pub Date : 2025-02-19 DOI: 10.1016/j.carpta.2025.100722

Naghmeh Taghizadeh , Shohreh Ariaeenejad , Marzieh Ghollasi , Elaheh Motamedi

This study presents the synthesis of a highly efficient bio-based hydrogel nanocomposite for enzyme immobilization to enhance hydrolysis of poultry feed. A hydrogel matrix was developed using polyvinyl alcohol (PVA) and gum Arabic (GA) grafted with acrylic acid (AA) and reinforced with nanocellulose/Se nanoparticles (NC-Se). Selenium nanoparticles were green-synthesized via ascorbic acid-mediated reduction of sodium selenite and loaded onto nanocellulose extracted from cotton stalks. The NC-Se nanofiller was incorporated into a PVA-GA-AA matrix with glyceryl methacrylate (GMA) as a crosslinker, forming an NC-Se/Hydrogel nanocomposite through free-radical polymerization. This nanocomposite exhibited superior thermal stability, reduced pore size, and enhanced water absorbency compared to the pristine hydrogel. Immobilization of the xylanase enzyme PersiXyn8 onto both hydrogel carriers resulted in improved thermal stability, with activity retention of 100 % and 42 % at 80 °C, for the immobilized and free enzymes, respectively. Kinetic analysis demonstrated higher specific activity (Kcat) of the PersiXyn8@Hydrogel and PersiXyn8@NC-Se/Hydrogel (0.0107 and 0.012 mMmin⁻¹mg⁻¹, respectively), compared to free PersiXyn8 (0.0105), indicating favorable interactions which could stabilize the active enzyme's conformation in hydrogel matrix. Immobilized enzymes showed excellent reusability (>50 % activity after six cycles) and storage stability, with 68.7 % activity retention for PersiXyn8@NC-Se/Hydrogel after 240 min. Furthermore, NC-Se/Hydrogel achieved significantly higher immobilization efficiency. When applied to poultry feed hydrolysis, PersiXyn8@NC-Se/Hydrogel doubled the release of reducing sugars and increased the water-holding capacity and solubility by 12-folds and over 2-folds, respectively, compared to the free enzyme. These findings highlight the potential of NC-Se/Hydrogel as a robust carrier for industrial enzyme applications.

{"title":"Development of polyvinyl alcohol-gum Arabic hydrogel nanocomposite for xylanase immobilization and its role in poultry feed enhancement","authors":"Naghmeh Taghizadeh , Shohreh Ariaeenejad , Marzieh Ghollasi , Elaheh Motamedi","doi":"10.1016/j.carpta.2025.100722","DOIUrl":"10.1016/j.carpta.2025.100722","url":null,"abstract":"<div><div>This study presents the synthesis of a highly efficient bio-based hydrogel nanocomposite for enzyme immobilization to enhance hydrolysis of poultry feed. A hydrogel matrix was developed using polyvinyl alcohol (PVA) and gum Arabic (GA) grafted with acrylic acid (AA) and reinforced with nanocellulose/Se nanoparticles (NC-Se). Selenium nanoparticles were green-synthesized via ascorbic acid-mediated reduction of sodium selenite and loaded onto nanocellulose extracted from cotton stalks. The NC-Se nanofiller was incorporated into a PVA-GA-AA matrix with glyceryl methacrylate (GMA) as a crosslinker, forming an NC-Se/Hydrogel nanocomposite through free-radical polymerization. This nanocomposite exhibited superior thermal stability, reduced pore size, and enhanced water absorbency compared to the pristine hydrogel. Immobilization of the xylanase enzyme PersiXyn8 onto both hydrogel carriers resulted in improved thermal stability, with activity retention of 100 % and 42 % at 80 °C, for the immobilized and free enzymes, respectively. Kinetic analysis demonstrated higher specific activity (Kcat) of the PersiXyn8@Hydrogel and PersiXyn8@NC-Se/Hydrogel (0.0107 and 0.012 mMmin<sup>−1</sup>mg<sup>−1</sup>, respectively), compared to free PersiXyn8 (0.0105), indicating favorable interactions which could stabilize the active enzyme's conformation in hydrogel matrix. Immobilized enzymes showed excellent reusability (>50 % activity after six cycles) and storage stability, with 68.7 % activity retention for PersiXyn8@NC-Se/Hydrogel after 240 min. Furthermore, NC-Se/Hydrogel achieved significantly higher immobilization efficiency. When applied to poultry feed hydrolysis, PersiXyn8@NC-Se/Hydrogel doubled the release of reducing sugars and increased the water-holding capacity and solubility by 12-folds and over 2-folds, respectively, compared to the free enzyme. These findings highlight the potential of NC-Se/Hydrogel as a robust carrier for industrial enzyme applications.</div></div>","PeriodicalId":100213,"journal":{"name":"Carbohydrate Polymer Technologies and Applications","volume":"9 ","pages":"Article 100722"},"PeriodicalIF":6.2,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0