从蜜蜂胃中分离的乳酸菌的益生潜力:功能和技术启示

IF 3.5 2区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Annals of Agricultural Science Pub Date : 2024-06-01 DOI:10.1016/j.aoas.2024.06.001
Mohamed G. Shehata , Saad H.D. Masry , Nourhan M. Abd El-Aziz , Fouad L. Ridouane , Shaher B. Mirza , Sobhy A. El-Sohaimy
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引用次数: 0

摘要

益生乳酸菌(LAB)因其潜在的健康益处而备受关注,特别是在支持肠道微生物群平衡方面。本研究试图通过评估蜜蜂胃中的 LAB 分离物对酸和胆汁的耐受性、自聚集性、疏水性、与病原体的共聚集性、抗氧化活性、溶血作用、外多糖(EPS)的产生、体外细胞粘附性及其在以牛奶为基础的发酵产品中的表现,来评估其作为潜在益生菌的价值。LAB 分离物对胃酸的耐受性令人印象深刻,在 pH 值为 2 的模拟胃液中培养 2 小时后仍能存活。在几种 LAB 分离物中观察到了对益生菌粘附到肠道上皮细胞至关重要的自聚集和疏水性。值得注意的是,Ehb3、Ehb5 和 Ehb8 的值最高,表明它们具有有效粘附肠道的潜力。使用 DPPH(2,2-二苯基-1-苦基肼)和 ABTS(2,2′-偶氮-双(3-乙基苯并噻唑啉-6-磺酸))自由基清除试验评估了细胞内和无细胞乳酸菌菌株提取物的抗氧化活性。Ehb3 和 Ehb5 表现出了卓越的抗氧化能力,这表明它们具有提高益生菌产品保质期和健康益处的潜力。这些乳酸菌株还能很好地发酵牛奶,在储存期间保持高于益生菌产品技术要求的活力。最后,从蜜蜂胃中分离出的乳酸菌表现出良好的特性,使其成为具有健康益处的潜在益生菌的合适候选菌株。
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Probiotic potential of lactic acid bacteria isolated from honeybees stomach: Functional and technological insights

Probiotic lactic acid bacteria (LAB) have garnered substantial attention for their potential health benefits, particularly in supporting the balance of gut microbiota. This study sought to assess LAB isolates from honeybees stomach as potential probiotics by evaluating their tolerance to acid and bile, autoaggregation, hydrophobicity, co-aggregation with pathogens, antioxidant activity, haemolysis, exopolysaccharide (EPS) production, in vitro cell adherence, and their performance in milk-based fermented products. The LAB isolates exhibited impressive resilience to gastric acid, surviving exposure to simulated gastric juice at pH 2 after 2 h of incubation. Autoaggregation and hydrophobicity, crucial for probiotic adhesion to intestinal epithelial cells, were observed in several LAB isolates. Notably, Ehb3, Ehb5, and Ehb8 displayed the highest values, indicating their potential for effective intestinal adhesion. The antioxidant activities of intracellular and cell-free lactic acid bacteria strain extracts were evaluated using DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging tests. Ehb3 and Ehb5 demonstrated outstanding antioxidant capabilities, suggesting their potential for enhancing the shelf life and health benefits of probiotic products. These lactic acid bacteria strains were also proficient in fermenting milk, maintaining viability above the technological requirements for probiotic products during storage. Finally, lactic acid bacteria isolate from honey bee stomach exhibit promising characteristics that make them suitable candidates for potential probiotics with health benefits.

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来源期刊
Annals of Agricultural Science
Annals of Agricultural Science AGRICULTURE, MULTIDISCIPLINARY-
CiteScore
12.60
自引率
0.00%
发文量
18
审稿时长
33 days
期刊介绍: Annals of Agricultural Sciences (AOAS) is the official journal of Faculty of Agriculture, Ain Shams University. AOAS is an open access peer-reviewed journal publishing original research articles and review articles on experimental and modelling research at laboratory, field, farm, landscape, and industrial levels. AOAS aims to maximize the quality of the agricultural sector across the globe with emphasis on the Arabian countries by focusing on publishing the high-quality applicable researches, in addition to the new methods and frontiers leading to maximizing the quality and quantity of both plant and animal yield and final products.
期刊最新文献
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