Shuai Huang , Jian-Zhu Wang , Yang-Li Pu , Yu-Yan Liu , Ying Chen , Lin Chen , Xian-Li Zhou
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引用次数: 0
摘要
通过对 Delphinium trichophorum Franch 全株的乙醇提取物进行植物化学研究，分离出了 10 种前所未有的二萜生物碱，其中包括 9 种名为 trichophodines A-I 的 delnudine 型 C20 二萜生物碱和 1 种名为 trichophozine A 的 kusnezoline 型 C20 二萜生物碱，此外还鉴定出了 7 种已知化合物。通过大量光谱分析，包括 HSQC、HMBC、1H-1H COSY、NOESY 和 X 射线晶体分析，阐明了这些化合物的结构。对大多数分离出的化合物进行了筛选，以检测其对 RAW 264.7 巨噬细胞中 LPS 诱导的 NO 生成的抑制活性以及对乙酰胆碱酯酶的抑制作用。Guan-fu base V 对乙酰胆碱酯酶有很强的抑制活性，在浓度为 40 μM 时抑制率为 53.81%。
Diterpenoid alkaloids from Delphinium trichophorum
The ethanol extract of the whole plant of Delphinium trichophorum Franch was subjected to a phytochemical study, leading to the isolation of ten unprecedented diterpenoid alkaloids, including nine delnudine-type C20-diterpenoid alkaloids named trichophodines A–I and one kusnezoline-type C20-diterpenoid alkaloid named trichophozine A. Additionally, seven known compounds were also identified. Their structures were elucidated on the basis of extensive spectroscopic analysis, including HSQC, HMBC, 1H–1H COSY, NOESY and X-ray crystallographic analysis. Most isolated compounds were screened for inhibitory activities against LPS-induced NO production in RAW 264.7 macrophage cells and acetylcholinesterase inhibitory effects. Guan-fu base V exhibited potent inhibitory activity against acetylcholinesterase, demonstrating an inhibitory rate of 53.81% at a concentration of 40 μM.
期刊介绍：
Phytochemistry is a leading international journal publishing studies of plant chemistry, biochemistry, molecular biology and genetics, structure and bioactivities of phytochemicals, including ''-omics'' and bioinformatics/computational biology approaches. Phytochemistry is a primary source for papers dealing with phytochemicals, especially reports concerning their biosynthesis, regulation, and biological properties both in planta and as bioactive principles. Articles are published online as soon as possible as Articles-in-Press and in 12 volumes per year. Occasional topic-focussed special issues are published composed of papers from invited authors.
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