Hasan Ejaz, Muhammad Usman Qamar, Aisha Farhana, Sonia Younas, Alia Batool, Durreshahwar Lone, Muhammad Atif, Mashael W. Alruways, Muharib Alruwaili, Ismail Hamad, Samy Selim, Bi Bi Zainab Mazhari, Ali Farooq, Kashaf Junaid
{"title":"抗生素耐药性的浪潮：对广谱β-内酰胺酶和碳青霉烯耐药大肠埃希菌和肺炎克雷伯菌的研究。","authors":"Hasan Ejaz, Muhammad Usman Qamar, Aisha Farhana, Sonia Younas, Alia Batool, Durreshahwar Lone, Muhammad Atif, Mashael W. Alruways, Muharib Alruwaili, Ismail Hamad, Samy Selim, Bi Bi Zainab Mazhari, Ali Farooq, Kashaf Junaid","doi":"10.1002/jcla.25081","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>The global spread of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Enterobacterales (CRE) poses a significant concern. Acquisition of antimicrobial resistance genes leads to resistance against several antibiotics, limiting treatment options. We aimed to study ESBL-producing and CRE transmission in clinical settings.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>From clinical samples, 227 ESBL-producing and CRE isolates were obtained. The isolates were cultured on bacterial media and confirmed by VITEK 2. Antibiograms were tested against several antibiotics using VITEK 2. The acquired resistance genes were identified by PCR.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Of the 227 clinical isolates, 145 (63.8%) were <i>Klebsiella pneumoniae</i> and 82 (36.1%) were <i>Escherichia coli</i>; 76 (33.4%) isolates were detected in urine, 57 (25.1%) in pus swabs, and 53 (23.3%) in blood samples. A total of 58 (70.7%) ESBL-producing <i>E. coli</i> were resistant to beta-lactams, except for carbapenems, and 17.2% were amikacin-resistant; 29.2% of <i>E. coli</i> isolates were resistant to carbapenems. A total of 106 (73.1%) ESBL-producing <i>K. pneumoniae</i> were resistant to all beta-lactams, except for carbapenems, and 66.9% to ciprofloxacin; 38 (26.2%) <i>K. pneumoniae</i> were resistant to carbapenems. Colistin emerged as the most effective antibiotic against both bacterial types. Twelve (20.6%) <i>E. coli</i> isolates were positive for <i>bla</i><sub>CTX-M</sub>, 11 (18.9%) for <i>bla</i><sub>TEM</sub>, and 8 (33.3%) for <i>bla</i><sub>NDM</sub>. Forty-six (52.3%) <i>K. pneumoniae</i> isolates had <i>bla</i><sub>CTX-M</sub>, 27 (18.6%) <i>bla</i><sub>TEM</sub>, and 26 (68.4%) <i>bla</i><sub>NDM</sub>.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>This study found a high prevalence of drug-resistant ESBL-producing and CRE, highlighting the need for targeted antibiotic use to combat resistance.</p>\n </section>\n </div>","PeriodicalId":15509,"journal":{"name":"Journal of Clinical Laboratory Analysis","volume":"38 10","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211664/pdf/","citationCount":"0","resultStr":"{\"title\":\"The Rising Tide of Antibiotic Resistance: A Study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae\",\"authors\":\"Hasan Ejaz, Muhammad Usman Qamar, Aisha Farhana, Sonia Younas, Alia Batool, Durreshahwar Lone, Muhammad Atif, Mashael W. 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The acquired resistance genes were identified by PCR.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Of the 227 clinical isolates, 145 (63.8%) were <i>Klebsiella pneumoniae</i> and 82 (36.1%) were <i>Escherichia coli</i>; 76 (33.4%) isolates were detected in urine, 57 (25.1%) in pus swabs, and 53 (23.3%) in blood samples. A total of 58 (70.7%) ESBL-producing <i>E. coli</i> were resistant to beta-lactams, except for carbapenems, and 17.2% were amikacin-resistant; 29.2% of <i>E. coli</i> isolates were resistant to carbapenems. A total of 106 (73.1%) ESBL-producing <i>K. pneumoniae</i> were resistant to all beta-lactams, except for carbapenems, and 66.9% to ciprofloxacin; 38 (26.2%) <i>K. pneumoniae</i> were resistant to carbapenems. Colistin emerged as the most effective antibiotic against both bacterial types. Twelve (20.6%) <i>E. coli</i> isolates were positive for <i>bla</i><sub>CTX-M</sub>, 11 (18.9%) for <i>bla</i><sub>TEM</sub>, and 8 (33.3%) for <i>bla</i><sub>NDM</sub>. Forty-six (52.3%) <i>K. pneumoniae</i> isolates had <i>bla</i><sub>CTX-M</sub>, 27 (18.6%) <i>bla</i><sub>TEM</sub>, and 26 (68.4%) <i>bla</i><sub>NDM</sub>.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>This study found a high prevalence of drug-resistant ESBL-producing and CRE, highlighting the need for targeted antibiotic use to combat resistance.</p>\\n </section>\\n </div>\",\"PeriodicalId\":15509,\"journal\":{\"name\":\"Journal of Clinical Laboratory Analysis\",\"volume\":\"38 10\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11211664/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Clinical Laboratory Analysis\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/jcla.25081\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Clinical Laboratory Analysis","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jcla.25081","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
The Rising Tide of Antibiotic Resistance: A Study on Extended-Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia coli and Klebsiella pneumoniae
Background
The global spread of extended-spectrum beta-lactamase (ESBL)-producing and carbapenem-resistant Enterobacterales (CRE) poses a significant concern. Acquisition of antimicrobial resistance genes leads to resistance against several antibiotics, limiting treatment options. We aimed to study ESBL-producing and CRE transmission in clinical settings.
Methods
From clinical samples, 227 ESBL-producing and CRE isolates were obtained. The isolates were cultured on bacterial media and confirmed by VITEK 2. Antibiograms were tested against several antibiotics using VITEK 2. The acquired resistance genes were identified by PCR.
Results
Of the 227 clinical isolates, 145 (63.8%) were Klebsiella pneumoniae and 82 (36.1%) were Escherichia coli; 76 (33.4%) isolates were detected in urine, 57 (25.1%) in pus swabs, and 53 (23.3%) in blood samples. A total of 58 (70.7%) ESBL-producing E. coli were resistant to beta-lactams, except for carbapenems, and 17.2% were amikacin-resistant; 29.2% of E. coli isolates were resistant to carbapenems. A total of 106 (73.1%) ESBL-producing K. pneumoniae were resistant to all beta-lactams, except for carbapenems, and 66.9% to ciprofloxacin; 38 (26.2%) K. pneumoniae were resistant to carbapenems. Colistin emerged as the most effective antibiotic against both bacterial types. Twelve (20.6%) E. coli isolates were positive for blaCTX-M, 11 (18.9%) for blaTEM, and 8 (33.3%) for blaNDM. Forty-six (52.3%) K. pneumoniae isolates had blaCTX-M, 27 (18.6%) blaTEM, and 26 (68.4%) blaNDM.
Conclusion
This study found a high prevalence of drug-resistant ESBL-producing and CRE, highlighting the need for targeted antibiotic use to combat resistance.
期刊介绍：
Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.
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