S. Purushothaman , P. Azhahianambi , M. Dharman , R. Gokula Kannan , K.G. Tirumurugaan , C. Soundararajan , R.P. Aravindh Babu , Panneer Devaraju , John A.J. Prakash
{"title":"关于南印度家庭鼠群中恙虫病分子流行率的横断面研究。","authors":"S. Purushothaman , P. Azhahianambi , M. Dharman , R. Gokula Kannan , K.G. Tirumurugaan , C. Soundararajan , R.P. Aravindh Babu , Panneer Devaraju , John A.J. Prakash","doi":"10.1016/j.cimid.2024.102212","DOIUrl":null,"url":null,"abstract":"<div><p>This study aimed to assess the molecular prevalence of mite-borne zoonotic pathogen <em>O. tsutsugamushi</em> in household rats of South India through nested polymerase chain reaction amplification of <em>O. tsutsugamushi</em> 47-kDa <em>htrA</em> gene and to determine the most suitable sample type for screening of <em>O. tsutsugamushi</em> in rats<em>.</em> Out of 85 rats trapped in Tamil Nadu, Karnataka, and Puducherry regions, 47 rats were found positive for the <em>O. tsutsugamushi</em> genome with prevalence of 55.29 %. Among different sample types screened, faecal samples exhibited the highest positivity rate, followed by liver, spleen, kidney, and blood samples. Agreement between faecal and spleen samples of rats for the presence of <em>O. tsutsugamushi</em> was the highest. Principal component analysis revealed a positive correlation between the spleen, liver, and faeces and a negative correlation between blood and faeces for the presence of <em>O. tsutsugamushi</em> genome. These findings underscore the varied distribution of <em>O. tsutsugamushi</em> among different samples and indicate that the faecal and liver samples of rats are an ideal choice of samples for epidemiological studies. This is the first study to report a high level of presence of <em>O. tsutsugamushi</em> in faecal samples of rats.</p></div>","PeriodicalId":50999,"journal":{"name":"Comparative Immunology Microbiology and Infectious Diseases","volume":"111 ","pages":"Article 102212"},"PeriodicalIF":2.0000,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A cross sectional study on molecular prevalence of Orientia tsutsugamushi in household rat population of South India\",\"authors\":\"S. Purushothaman , P. Azhahianambi , M. Dharman , R. Gokula Kannan , K.G. Tirumurugaan , C. Soundararajan , R.P. Aravindh Babu , Panneer Devaraju , John A.J. Prakash\",\"doi\":\"10.1016/j.cimid.2024.102212\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>This study aimed to assess the molecular prevalence of mite-borne zoonotic pathogen <em>O. tsutsugamushi</em> in household rats of South India through nested polymerase chain reaction amplification of <em>O. tsutsugamushi</em> 47-kDa <em>htrA</em> gene and to determine the most suitable sample type for screening of <em>O. tsutsugamushi</em> in rats<em>.</em> Out of 85 rats trapped in Tamil Nadu, Karnataka, and Puducherry regions, 47 rats were found positive for the <em>O. tsutsugamushi</em> genome with prevalence of 55.29 %. Among different sample types screened, faecal samples exhibited the highest positivity rate, followed by liver, spleen, kidney, and blood samples. Agreement between faecal and spleen samples of rats for the presence of <em>O. tsutsugamushi</em> was the highest. Principal component analysis revealed a positive correlation between the spleen, liver, and faeces and a negative correlation between blood and faeces for the presence of <em>O. tsutsugamushi</em> genome. These findings underscore the varied distribution of <em>O. tsutsugamushi</em> among different samples and indicate that the faecal and liver samples of rats are an ideal choice of samples for epidemiological studies. This is the first study to report a high level of presence of <em>O. tsutsugamushi</em> in faecal samples of rats.</p></div>\",\"PeriodicalId\":50999,\"journal\":{\"name\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"volume\":\"111 \",\"pages\":\"Article 102212\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Immunology Microbiology and Infectious Diseases\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147957124000894\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Immunology Microbiology and Infectious Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147957124000894","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
A cross sectional study on molecular prevalence of Orientia tsutsugamushi in household rat population of South India
This study aimed to assess the molecular prevalence of mite-borne zoonotic pathogen O. tsutsugamushi in household rats of South India through nested polymerase chain reaction amplification of O. tsutsugamushi 47-kDa htrA gene and to determine the most suitable sample type for screening of O. tsutsugamushi in rats. Out of 85 rats trapped in Tamil Nadu, Karnataka, and Puducherry regions, 47 rats were found positive for the O. tsutsugamushi genome with prevalence of 55.29 %. Among different sample types screened, faecal samples exhibited the highest positivity rate, followed by liver, spleen, kidney, and blood samples. Agreement between faecal and spleen samples of rats for the presence of O. tsutsugamushi was the highest. Principal component analysis revealed a positive correlation between the spleen, liver, and faeces and a negative correlation between blood and faeces for the presence of O. tsutsugamushi genome. These findings underscore the varied distribution of O. tsutsugamushi among different samples and indicate that the faecal and liver samples of rats are an ideal choice of samples for epidemiological studies. This is the first study to report a high level of presence of O. tsutsugamushi in faecal samples of rats.
期刊介绍：
Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine .
The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
