突变育种及其在现代植物育种中的重要性:综述

Shahzeb Ali, Talekar Nilesh Suryakant
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摘要

突变是指活细胞 DNA 突然发生的、可遗传的改变,这种改变不是由基因重组或分离引起的。在植物育种中有意识地使用突变被称为 "突变育种"。与杂交和选育相比,突变育种的优势在于可以在不牺牲农艺性状和品质特征的前提下,改良一个原本优秀的栽培品种的缺点。要改良无籽作物,没有比突变育种更简单的办法了。自欧洲最初发布源自基础突变研究的突变栽培品种以来,这些优势已导致植物育种领域突变育种市场的发展。物理诱变剂和化学诱变剂改进了诱导主要作物突变的方法,并详细介绍了突变种群的选择策略。目前已经发现了许多以前没有记录的突变,并且正在研究新的诱变因素,如宇宙射线和离子束辐射。不过,电离辐射和烷基化化学品仍在广泛使用。由于出现了适用于许多作物物种的可靠体外方法,突变体培育的效率得到了提高。体外方法特别有效,因为它们可以在小范围内管理相当大的诱变种群,在无性繁殖物种中具有较快的后代周转率,并且可以筛选培养环境中的各种生物和非生物胁迫因素。在过去的十年中,突变体筛选技术有了长足的进步,反向遗传方法已成为优先考虑的方法。因此,分子方法和突变技术的结合为当代植物育种开辟了新的迷人的可能性。
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Mutation Breeding and Its Importance in Modern Plant Breeding: A Review
A mutation is an abrupt, heritable alteration in a living cell's DNA that is not brought about by genetic recombination or segregation. The deliberate use of mutations in plant breeding is known as "mutation breeding." Mutation breeding provides the advantage of improving a fault in an otherwise excellent cultivar without sacrificing its agronomic and qualitative features, in contrast to hybridization and selection. There is no simpler solution than mutation breeding to enhance seedless crops. These benefits have led to the development of a market for mutation breeding in plant breeding since the initial release of mutant cultivars derived from fundamental mutation research in Europe. Both physical and chemical mutagens have improved methods for inducing mutations in major crops, and strategies for selecting mutant populations have been detailed. A broad range of mutations that have not been previously documented have been detected, and new mutagenic factors like cosmic rays and ion beam radiation are being studied. However, ionising radiation and alkylating chemicals continue to be widely used. The efficiency of mutant breeding has increased as a result of the advent of reliable in vitro methods for numerous crop species. In vitro methods are particularly effective because they can manage sizable mutagenized populations in a small area, have a quicker progeny turnover rate in vegetatively propagated species, and can screen for a variety of biotic and abiotic stress factors in the culture environment. Over the last ten years, there have been significant advancements in mutant screening, with reverse genetic methods now being prioritised. Thus, the combination of molecular methods and mutation techniques is opening up new and intriguing possibilities for contemporary plant breeding.
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