探索隐藏的宝藏:辣椒田杂草根瘤中磷酸盐溶解细菌 (PSB) 的分离、特征描述和植物生长促进性状筛选

Vinuthana V H, Yallappa M, Mahadevaswamy, Pampanagouda, Savita A S
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引用次数: 0

摘要

植物相关微生物会影响植物的生长、养分获取、对不同胁迫条件的耐受性等。然而,影响杂草生物合成的有益微生物在很大程度上仍未得到探索和利用。磷是辣椒植株生长和发育所必需的主要矿物质之一。本研究的主要目的是从辣椒生态系统的杂草根瘤中分离出 PSB,并对其 PGPR 特性进行评估。共从辣椒生态系统中的不同杂草中分离出 40 个 PSB 分离物,并对其进行了生化鉴定,发现 13 个分离物在淀粉水解方面呈阳性,35 个分离物在产酸方面呈阳性,12 个分离物在产气方面呈阳性,40 个分离物在过氧化氢酶生成方面呈阳性,5 个分离物在 H2S 生成方面呈阳性,13 个分离物在脲酶生成方面呈阳性,其余所有分离物在相应测试中均呈阴性。根据形态和生化特征,初步确定分离物为芽孢杆菌和假单胞菌。
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Exploring the Hidden Treasures: Isolation, Characterization and Screening of Phosphate Solubilizing Bacteria (PSB) for Plant Growth-Promoting Traits from Chilli Field Weeds Rhizosphere
Plant-associated microbes affect growth, nutrient acquisition, tolerance to different stress conditions and etc. by the plant. However, beneficial microbes influencing weeds biosynthesis remain largely unexplored and unexploited. Phosphorus is one of essential macro-minerals for the growth and development of chilli plants. The main objective of this study was to isolate characterize and evaluation of the PGPR characters of PSB from weed rhizosphere of the chilli ecosystem. Totally 40 PSB isolates were isolated from different weeds in the chilli ecosystem and were subjected to biochemical characterization where 13 isolates were found positive for starch hydrolysis, 35 isolates were positive for acid production, 12isolates were positive for gas production, 40 isolates were positive for catalase production, 5 isolates were positive for H2S production, 13 isolates were positive for urease production and remaining all isolated were negative for the respective tests. Based on morphological and biochemical characterization, the isolates were tentatively identified as Bacillus sp and Pseudomonas sp.
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