Lucas G. Huggins , Ugyen Namgyel , Pelden Wangchuk , Ushani Atapattu , Rebecca Traub , Vito Colella
{"title":"利用纳米孔测序技术进行元条形编码可鉴定来自被忽视地区的多种人畜共患病病原体:调查不丹狗的案例研究","authors":"Lucas G. Huggins , Ugyen Namgyel , Pelden Wangchuk , Ushani Atapattu , Rebecca Traub , Vito Colella","doi":"10.1016/j.onehlt.2024.100839","DOIUrl":null,"url":null,"abstract":"<div><p>The diversity and prevalence of canine vector-borne pathogens (VBPs) in Bhutan have to date remained unexplored, whilst recent epidemiological surveys in other South Asian nations have found diseases caused by VBPs to be rife in local dog populations. Importantly, many of such VBPs can infect people as well, with a building body of evidence identifying potentially zoonotic rickettsial organisms infecting humans in Bhutan. Given the lack of data on canine pathogens in Bhutan we employed a suite of deep-sequencing metabarcoding methods using Oxford Nanopore Technologies' MinION™ device to holistically characterise the bacterial, apicomplexan and filarial worm blood-borne pathogens of dogs in the country's south. Of the 95 stray, owned and community dogs sampled 78% (95% CI = 69%–85%) were infected with at least one VBP. Pathogen species detected were highly diverse including the bacteria <em>Mycoplasma haemocanis</em> in 16% (95% CI: 10–24%), <em>Ehrlichia canis</em> in 4% (95% CI: 2–10%), <em>Anaplasma platys</em> in 2% (95% CI: 0.5–7%) of dogs as well as the zoonotic species <em>Bartonella clarridgeiae</em> in 1% (95% CI: 0.1–6%), a potentially novel <em>Bartonella</em> spp. and an <em>Ehrlichia chaffeensis</em>-like bacterium, both in 1% (95% CI: 0.1–6%) of dogs. The apicomplexan haemoparasites <em>Hepatozoon canis</em> in 62% (95% CI: 52–71%), <em>Babesia gibsoni</em> in 45% (95% CI: 36–55%) and <em>Babesia vogeli</em> in 3% (95% CI: 1–9%) of dogs were also detected. Finally, 5% (95% CI: 2–12%) of dogs were found to be infected with the filarioid <em>Acanthocheilonema reconditum</em> and 1% (95% CI: 0.1–6%) with zoonotic <em>Dirofilaria</em> sp. hongkongensis. One canine was found positive to the filarioid <em>Setaria tundra</em>, a species normally found infecting cervids. The elucidated diversity of VBP communities highlights the strength of assumption-free diagnostics, such as metabarcoding, in detecting rare, novel, and unexpected pathogens. This approach to identifying pathogen diversity is of critical importance when investigating regions and populations that have thus far been neglected, with the findings aiding the development of future One Health informed strategies for disease control.</p></div>","PeriodicalId":19577,"journal":{"name":"One Health","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2352771424001654/pdfft?md5=62f10dc07e5b6b7bdf70d9d01ec2804c&pid=1-s2.0-S2352771424001654-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Metabarcoding using nanopore sequencing enables identification of diverse and zoonotic vector-borne pathogens from neglected regions: A case study investigating dogs from Bhutan\",\"authors\":\"Lucas G. Huggins , Ugyen Namgyel , Pelden Wangchuk , Ushani Atapattu , Rebecca Traub , Vito Colella\",\"doi\":\"10.1016/j.onehlt.2024.100839\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The diversity and prevalence of canine vector-borne pathogens (VBPs) in Bhutan have to date remained unexplored, whilst recent epidemiological surveys in other South Asian nations have found diseases caused by VBPs to be rife in local dog populations. Importantly, many of such VBPs can infect people as well, with a building body of evidence identifying potentially zoonotic rickettsial organisms infecting humans in Bhutan. Given the lack of data on canine pathogens in Bhutan we employed a suite of deep-sequencing metabarcoding methods using Oxford Nanopore Technologies' MinION™ device to holistically characterise the bacterial, apicomplexan and filarial worm blood-borne pathogens of dogs in the country's south. Of the 95 stray, owned and community dogs sampled 78% (95% CI = 69%–85%) were infected with at least one VBP. Pathogen species detected were highly diverse including the bacteria <em>Mycoplasma haemocanis</em> in 16% (95% CI: 10–24%), <em>Ehrlichia canis</em> in 4% (95% CI: 2–10%), <em>Anaplasma platys</em> in 2% (95% CI: 0.5–7%) of dogs as well as the zoonotic species <em>Bartonella clarridgeiae</em> in 1% (95% CI: 0.1–6%), a potentially novel <em>Bartonella</em> spp. and an <em>Ehrlichia chaffeensis</em>-like bacterium, both in 1% (95% CI: 0.1–6%) of dogs. The apicomplexan haemoparasites <em>Hepatozoon canis</em> in 62% (95% CI: 52–71%), <em>Babesia gibsoni</em> in 45% (95% CI: 36–55%) and <em>Babesia vogeli</em> in 3% (95% CI: 1–9%) of dogs were also detected. Finally, 5% (95% CI: 2–12%) of dogs were found to be infected with the filarioid <em>Acanthocheilonema reconditum</em> and 1% (95% CI: 0.1–6%) with zoonotic <em>Dirofilaria</em> sp. hongkongensis. One canine was found positive to the filarioid <em>Setaria tundra</em>, a species normally found infecting cervids. The elucidated diversity of VBP communities highlights the strength of assumption-free diagnostics, such as metabarcoding, in detecting rare, novel, and unexpected pathogens. This approach to identifying pathogen diversity is of critical importance when investigating regions and populations that have thus far been neglected, with the findings aiding the development of future One Health informed strategies for disease control.</p></div>\",\"PeriodicalId\":19577,\"journal\":{\"name\":\"One Health\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.1000,\"publicationDate\":\"2024-06-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2352771424001654/pdfft?md5=62f10dc07e5b6b7bdf70d9d01ec2804c&pid=1-s2.0-S2352771424001654-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"One Health\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2352771424001654\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"One Health","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352771424001654","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
Metabarcoding using nanopore sequencing enables identification of diverse and zoonotic vector-borne pathogens from neglected regions: A case study investigating dogs from Bhutan
The diversity and prevalence of canine vector-borne pathogens (VBPs) in Bhutan have to date remained unexplored, whilst recent epidemiological surveys in other South Asian nations have found diseases caused by VBPs to be rife in local dog populations. Importantly, many of such VBPs can infect people as well, with a building body of evidence identifying potentially zoonotic rickettsial organisms infecting humans in Bhutan. Given the lack of data on canine pathogens in Bhutan we employed a suite of deep-sequencing metabarcoding methods using Oxford Nanopore Technologies' MinION™ device to holistically characterise the bacterial, apicomplexan and filarial worm blood-borne pathogens of dogs in the country's south. Of the 95 stray, owned and community dogs sampled 78% (95% CI = 69%–85%) were infected with at least one VBP. Pathogen species detected were highly diverse including the bacteria Mycoplasma haemocanis in 16% (95% CI: 10–24%), Ehrlichia canis in 4% (95% CI: 2–10%), Anaplasma platys in 2% (95% CI: 0.5–7%) of dogs as well as the zoonotic species Bartonella clarridgeiae in 1% (95% CI: 0.1–6%), a potentially novel Bartonella spp. and an Ehrlichia chaffeensis-like bacterium, both in 1% (95% CI: 0.1–6%) of dogs. The apicomplexan haemoparasites Hepatozoon canis in 62% (95% CI: 52–71%), Babesia gibsoni in 45% (95% CI: 36–55%) and Babesia vogeli in 3% (95% CI: 1–9%) of dogs were also detected. Finally, 5% (95% CI: 2–12%) of dogs were found to be infected with the filarioid Acanthocheilonema reconditum and 1% (95% CI: 0.1–6%) with zoonotic Dirofilaria sp. hongkongensis. One canine was found positive to the filarioid Setaria tundra, a species normally found infecting cervids. The elucidated diversity of VBP communities highlights the strength of assumption-free diagnostics, such as metabarcoding, in detecting rare, novel, and unexpected pathogens. This approach to identifying pathogen diversity is of critical importance when investigating regions and populations that have thus far been neglected, with the findings aiding the development of future One Health informed strategies for disease control.
期刊介绍:
One Health - a Gold Open Access journal.
The mission of One Health is to provide a platform for rapid communication of high quality scientific knowledge on inter- and intra-species pathogen transmission, bringing together leading experts in virology, bacteriology, parasitology, mycology, vectors and vector-borne diseases, tropical health, veterinary sciences, pathology, immunology, food safety, mathematical modelling, epidemiology, public health research and emergency preparedness. As a Gold Open Access journal, a fee is payable on acceptance of the paper. Please see the Guide for Authors for more information.
Submissions to the following categories are welcome:
Virology,
Bacteriology,
Parasitology,
Mycology,
Vectors and vector-borne diseases,
Co-infections and co-morbidities,
Disease spatial surveillance,
Modelling,
Tropical Health,
Discovery,
Ecosystem Health,
Public Health.