Rafael A.N. Ramos , Hassan Hakimi , Jordan Salomon , Rachel E. Busselman , Rachel Curtis-Robles , Carolyn L. Hodo , Sarah A. Hamer , Guilherme G. Verocai
{"title":"在美国得克萨斯州野生食肉动物体内检测到的密螺旋体和条纹盘尾丝虫（螺旋体：盘尾丝虫科","authors":"Rafael A.N. Ramos , Hassan Hakimi , Jordan Salomon , Rachel E. Busselman , Rachel Curtis-Robles , Carolyn L. Hodo , Sarah A. Hamer , Guilherme G. Verocai","doi":"10.1016/j.ijppaw.2024.100954","DOIUrl":null,"url":null,"abstract":"<div><p><em>Dirofilaria immitis</em> and <em>Dirofilaria striata</em> (Spirurida: Onchocercidae) are epidemiologically important filarial nematodes detected in wild carnivores sympatric to domestic animals and humans. In this study we surveyed for <em>Dirofilaria</em> species among previous studies archived blood samples (n = 202) of wild carnivores sourced across Texas between the years of 2014–2016 and 2020 to 2023. In total, 117 coyotes (<em>Canis latrans</em>), 67 raccoons (<em>Procyon lotor</em>), 12 gray foxes (<em>Urocyon cinereoargenteus</em>), five bobcats (<em>Lynx rufus</em>), and one striped skunk (<em>Mephitis mephitis</em>) were tested through the amplification of the partial cytochrome oxidase c subunit 1 (<em>COI</em>) gene followed by sequencing. <em>Dirofilaria immitis</em> was detected in 11.39% (95% CI = 7.71–16.51) of the samples (21 coyotes and two raccoons), while <em>D. striata</em> was detected in a single bobcat. <em>Dirofilaria immitis</em> sequences had 99.85%–100% (99.92% ± 0.08) similarity with other <em>D. immitis</em> sequences in GenBank. The sequence of <em>D. striata</em> from the bobcat was 100% similar to the single <em>COI</em> sequence available in GenBank. Data from this study reinforce the role of coyotes as a wild reservoir for <em>D. immitis</em> and suggest that raccoons may also play a role in the epidemiology of this parasite. This study additionally provides molecular data on <em>D. striata</em>, an understudied filarioid of felids.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100954"},"PeriodicalIF":2.0000,"publicationDate":"2024-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000506/pdfft?md5=d473871778a888b18273e10ea118efe0&pid=1-s2.0-S2213224424000506-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Dirofilaria immitis and Dirofilaria striata (Spirurida: Onchocercidae) detected in wild carnivores from Texas, United States\",\"authors\":\"Rafael A.N. Ramos , Hassan Hakimi , Jordan Salomon , Rachel E. Busselman , Rachel Curtis-Robles , Carolyn L. Hodo , Sarah A. Hamer , Guilherme G. Verocai\",\"doi\":\"10.1016/j.ijppaw.2024.100954\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><em>Dirofilaria immitis</em> and <em>Dirofilaria striata</em> (Spirurida: Onchocercidae) are epidemiologically important filarial nematodes detected in wild carnivores sympatric to domestic animals and humans. In this study we surveyed for <em>Dirofilaria</em> species among previous studies archived blood samples (n = 202) of wild carnivores sourced across Texas between the years of 2014–2016 and 2020 to 2023. In total, 117 coyotes (<em>Canis latrans</em>), 67 raccoons (<em>Procyon lotor</em>), 12 gray foxes (<em>Urocyon cinereoargenteus</em>), five bobcats (<em>Lynx rufus</em>), and one striped skunk (<em>Mephitis mephitis</em>) were tested through the amplification of the partial cytochrome oxidase c subunit 1 (<em>COI</em>) gene followed by sequencing. <em>Dirofilaria immitis</em> was detected in 11.39% (95% CI = 7.71–16.51) of the samples (21 coyotes and two raccoons), while <em>D. striata</em> was detected in a single bobcat. <em>Dirofilaria immitis</em> sequences had 99.85%–100% (99.92% ± 0.08) similarity with other <em>D. immitis</em> sequences in GenBank. The sequence of <em>D. striata</em> from the bobcat was 100% similar to the single <em>COI</em> sequence available in GenBank. Data from this study reinforce the role of coyotes as a wild reservoir for <em>D. immitis</em> and suggest that raccoons may also play a role in the epidemiology of this parasite. This study additionally provides molecular data on <em>D. striata</em>, an understudied filarioid of felids.</p></div>\",\"PeriodicalId\":54278,\"journal\":{\"name\":\"International Journal for Parasitology-Parasites and Wildlife\",\"volume\":\"24 \",\"pages\":\"Article 100954\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-06-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2213224424000506/pdfft?md5=d473871778a888b18273e10ea118efe0&pid=1-s2.0-S2213224424000506-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal for Parasitology-Parasites and Wildlife\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213224424000506\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ECOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal for Parasitology-Parasites and Wildlife","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213224424000506","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
Dirofilaria immitis and Dirofilaria striata (Spirurida: Onchocercidae) detected in wild carnivores from Texas, United States
Dirofilaria immitis and Dirofilaria striata (Spirurida: Onchocercidae) are epidemiologically important filarial nematodes detected in wild carnivores sympatric to domestic animals and humans. In this study we surveyed for Dirofilaria species among previous studies archived blood samples (n = 202) of wild carnivores sourced across Texas between the years of 2014–2016 and 2020 to 2023. In total, 117 coyotes (Canis latrans), 67 raccoons (Procyon lotor), 12 gray foxes (Urocyon cinereoargenteus), five bobcats (Lynx rufus), and one striped skunk (Mephitis mephitis) were tested through the amplification of the partial cytochrome oxidase c subunit 1 (COI) gene followed by sequencing. Dirofilaria immitis was detected in 11.39% (95% CI = 7.71–16.51) of the samples (21 coyotes and two raccoons), while D. striata was detected in a single bobcat. Dirofilaria immitis sequences had 99.85%–100% (99.92% ± 0.08) similarity with other D. immitis sequences in GenBank. The sequence of D. striata from the bobcat was 100% similar to the single COI sequence available in GenBank. Data from this study reinforce the role of coyotes as a wild reservoir for D. immitis and suggest that raccoons may also play a role in the epidemiology of this parasite. This study additionally provides molecular data on D. striata, an understudied filarioid of felids.
期刊介绍：
The International Journal for Parasitology: Parasites and Wildlife (IJP-PAW) publishes the results of original research on parasites of all wildlife, invertebrate and vertebrate. This includes free-ranging, wild populations, as well as captive wildlife, semi-domesticated species (e.g. reindeer) and farmed populations of recently domesticated or wild-captured species (e.g. cultured fishes). Articles on all aspects of wildlife parasitology are welcomed including taxonomy, biodiversity and distribution, ecology and epidemiology, population biology and host-parasite relationships. The impact of parasites on the health and conservation of wildlife is seen as an important area covered by the journal especially the potential role of environmental factors, for example climate. Also important to the journal is ''one health'' and the nature of interactions between wildlife, people and domestic animals, including disease emergence and zoonoses.
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