Ning Liu , Ziqiang Yin , Mingshuang Wang , Hongqian Kui , Zhenshuang Yuan , Yue Tian , Chuanxin Liu , Jianmei Huang
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引用次数: 0
摘要
复方雪莲通胶囊(FXT)对糖尿病视网膜病变(DR)有显著的预防和治疗作用,但其活性成分的相容性仍有待深入研究。本研究利用高混糖建立了斑马鱼糖尿病视网膜病变模型,并通过正交实验筛选出了田七总皂苷、丹参酚酸、黄芪皂苷、哈巴苷的最佳配比。此外,我们还采用UPLC-QqQ/MS检测DR斑马鱼组织在服用FXT及其配伍方后氨基酸含量的变化,分析FXT及其配伍方对氨基酸代谢产物的影响。结果表明,通过对各项指标的综合评价,各组分的最终相容比为 8:5:1:6.6。FXT及其配伍方对DR斑马鱼视网膜血管舒张、肝脏脂质蓄积、总葡萄糖、血管内皮生长因子水平均有促进作用,且均能唤回DR斑马鱼体内部分氨基酸水平。在这项研究中,我们确定了 FXT 中有效成分的兼容配方,并研究了它们在 DR 斑马鱼中的疗效,以便进一步应用于临床。
Pharmacodynamic and targeted amino acid metabolomics researches on the improvement of diabetic retinopathy with Fufang Xueshuantong component compatibility
The Fufang Xueshuantong capsule (FXT) has significant preventive and therapeutic effects on diabetic retinopathy(DR), but the compatibility of its active components remains to be thoroughly explored. In this study, a zebrafish diabetic retinopathy model was established using high-mixed sugars, and the optimal ratios of notoginseng total saponins, total salvianolic acid, astragaloside, and harpagide were selected through orthogonal experiments. Furthermore, we used UPLC-QqQ/MS to detect the changes in amino acid content of DR zebrafish tissues after administration of FXT and its compatible formula to analyze the effects of FXT and its compatible formula on amino acid metabolites. The results showed that the final compatibility ratios of the components were 8: 5: 1: 6.6 by comprehensive evaluation of the indicators. FXT and its compatibility formula had beneficial effects on retinal vasodilatation, lipid accumulation in the liver, total glucose, and VEGF levels in DR zebrafish, and all of them could call back some amino acid levels in DR zebrafish. In this research, we determined the compatible formulation of the active ingredients in the FXT and investigated their efficacy in DR zebrafish for further clinical applications.
期刊介绍:
The Journal of Chromatography B publishes papers on developments in separation science relevant to biology and biomedical research including both fundamental advances and applications. Analytical techniques which may be considered include the various facets of chromatography, electrophoresis and related methods, affinity and immunoaffinity-based methodologies, hyphenated and other multi-dimensional techniques, and microanalytical approaches. The journal also considers articles reporting developments in sample preparation, detection techniques including mass spectrometry, and data handling and analysis.
Developments related to preparative separations for the isolation and purification of components of biological systems may be published, including chromatographic and electrophoretic methods, affinity separations, field flow fractionation and other preparative approaches.
Applications to the analysis of biological systems and samples will be considered when the analytical science contains a significant element of novelty, e.g. a new approach to the separation of a compound, novel combination of analytical techniques, or significantly improved analytical performance.