来自 Phanerochaete chrysosporium 的 Cel45A 的 1H、13C 和 15N 主干共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS Biomolecular NMR Assignments Pub Date : 2024-06-18 DOI:10.1007/s12104-024-10182-6

Laura Okmane, Mats Sandgren, Jerry Ståhlberg, Gustav Nestor

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引用次数: 0

摘要

用 13C 和 15N 标记在 Pichia pastoris 中表达了来自白腐基枝菌 Phanerochaete chrysosporium 的糖苷水解酶家族 45（GH45）（PcCel45A）。利用 TALOS-N 软件对 1H、13C 和 15N 主干共振进行了近乎完整的分配，并根据分配的化学位移对二级结构进行了预测。除了序列末端的差异外，预测的二级结构与之前公布的同一种酶的晶体结构几乎完全相同。这是首次使用同位素标记的 GH45 酶进行核磁共振研究。

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1H, 13C and 15N backbone resonance assignment of Cel45A from Phanerochaete chrysosporium

A glycoside hydrolase family 45 (GH45) enzyme from the white-rot basidiomycete fungus Phanerochaete chrysosporium (PcCel45A) was expressed in Pichia pastoris with ¹³C and ¹⁵N labelling. A nearly complete assignment of ¹H, ¹³C and ¹⁵N backbone resonances was obtained, as well as the secondary structure prediction based on the assigned chemical shifts using the TALOS-N software. The predicted secondary structure was almost identical to previously published crystal structures of the same enzyme, except for differences in the termini of the sequence. This is the first NMR study using an isotopically labelled GH45 enzyme.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.