定量蛋白质组学揭示了 Sox 系统在光营养体卤虫的硫和砷代谢中的作用。

IF 4.3 2区 生物学 Q2 MICROBIOLOGY Environmental microbiology Pub Date : 2024-06-19 DOI:10.1111/1462-2920.16655
Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer-Friedrich, Corinne Casiot-Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp-Cothenet
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引用次数: 0

摘要

对紫色硫细菌无氧光合作用新陈代谢过程的研究主要集中在 Chromatiaceae 家族的 Allochromatium vinosum 上。然而,外硫华菌科(Ectothiorhodospiraceae)和卤代硫华菌科(Halorhodospiraceae)紫硫细菌的新陈代谢过程仍未得到研究。我们分析了用各种硫化合物培养的 Halorhodospira halophila 的蛋白质组。通过分析,我们首次为该家族重建了全面的硫氧化光合网络。有研究表明,Ectothiorhodospiraceae 家族的一些成员使用亚砷酸盐作为光合电子供体。因此,我们分析了 Halorhodospira halophila 在亚砷酸盐和硫化物条件下生长时蛋白质组的反应。我们使用离子色谱-电感耦合等离子体质谱法进行的分析表明,硫代砷酸盐会在这些条件下化学生成。然而,在有细菌存在的情况下,硫代砷酸盐的生成和转化更为广泛,这表明存在一个生物过程。我们的定量蛋白质组学发现,通常专门用于硫代硫酸盐氧化的 SoxAXYZB 系统在这些生长条件下过度产生。此外,细胞色素 c551/c5 和 HiPIP III 这两种电子载体也生产过剩。电子顺磁共振光谱表明,这些载体参与了光合作用反应中心的还原过程。这些结果支持这样一种观点,即化学和生物形成的硫代砷酸盐被 Sox 系统氧化,细胞色素 c551/c5 和 HiPIP III 将电子导向反应中心。
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Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila

The metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sulphur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome c551/c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551/c5 and HiPIP III directing electrons towards the Reaction Centre.

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来源期刊
Environmental microbiology
Environmental microbiology 环境科学-微生物学
CiteScore
9.90
自引率
3.90%
发文量
427
审稿时长
2.3 months
期刊介绍: Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens
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