Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer-Friedrich, Corinne Casiot-Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp-Cothenet
{"title":"定量蛋白质组学揭示了 Sox 系统在光营养体卤虫的硫和砷代谢中的作用。","authors":"Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer-Friedrich, Corinne Casiot-Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp-Cothenet","doi":"10.1111/1462-2920.16655","DOIUrl":null,"url":null,"abstract":"<p>The metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in <i>Allochromatium vinosum</i>, a member of the <i>Chromatiaceae</i> family. However, the metabolic processes of purple sulphur bacteria from the <i>Ectothiorhodospiraceae</i> and <i>Halorhodospiraceae</i> families remain unexplored. We have analysed the proteome of <i>Halorhodospira halophila</i>, a member of the <i>Halorhodospiraceae</i> family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the <i>Ectothiorhodospiraceae</i> family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of <i>Halorhodospira halophila</i> when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome <i>c</i><sub>551</sub>/<i>c</i><sub>5</sub> and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome <i>c</i><sub>551</sub>/<i>c</i><sub>5</sub> and HiPIP III directing electrons towards the Reaction Centre.</p>","PeriodicalId":11898,"journal":{"name":"Environmental microbiology","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16655","citationCount":"0","resultStr":"{\"title\":\"Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila\",\"authors\":\"Giulia D'Ermo, Stéphane Audebert, Luc Camoin, Britta Planer-Friedrich, Corinne Casiot-Marouani, Sophie Delpoux, Régine Lebrun, Marianne Guiral, Barbara Schoepp-Cothenet\",\"doi\":\"10.1111/1462-2920.16655\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in <i>Allochromatium vinosum</i>, a member of the <i>Chromatiaceae</i> family. However, the metabolic processes of purple sulphur bacteria from the <i>Ectothiorhodospiraceae</i> and <i>Halorhodospiraceae</i> families remain unexplored. We have analysed the proteome of <i>Halorhodospira halophila</i>, a member of the <i>Halorhodospiraceae</i> family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the <i>Ectothiorhodospiraceae</i> family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of <i>Halorhodospira halophila</i> when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome <i>c</i><sub>551</sub>/<i>c</i><sub>5</sub> and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome <i>c</i><sub>551</sub>/<i>c</i><sub>5</sub> and HiPIP III directing electrons towards the Reaction Centre.</p>\",\"PeriodicalId\":11898,\"journal\":{\"name\":\"Environmental microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1111/1462-2920.16655\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.16655\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.16655","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Quantitative proteomics reveals the Sox system's role in sulphur and arsenic metabolism of phototroph Halorhodospira halophila
The metabolic process of purple sulphur bacteria's anoxygenic photosynthesis has been primarily studied in Allochromatium vinosum, a member of the Chromatiaceae family. However, the metabolic processes of purple sulphur bacteria from the Ectothiorhodospiraceae and Halorhodospiraceae families remain unexplored. We have analysed the proteome of Halorhodospira halophila, a member of the Halorhodospiraceae family, which was cultivated with various sulphur compounds. This analysis allowed us to reconstruct the first comprehensive sulphur-oxidative photosynthetic network for this family. Some members of the Ectothiorhodospiraceae family have been shown to use arsenite as a photosynthetic electron donor. Therefore, we analysed the proteome response of Halorhodospira halophila when grown under arsenite and sulphide conditions. Our analyses using ion chromatography-inductively coupled plasma mass spectrometry showed that thioarsenates are chemically formed under these conditions. However, they are more extensively generated and converted in the presence of bacteria, suggesting a biological process. Our quantitative proteomics revealed that the SoxAXYZB system, typically dedicated to thiosulphate oxidation, is overproduced under these growth conditions. Additionally, two electron carriers, cytochrome c551/c5 and HiPIP III, are also overproduced. Electron paramagnetic resonance spectroscopy suggested that these transporters participate in the reduction of the photosynthetic Reaction Centre. These results support the idea of a chemically and biologically formed thioarsenate being oxidized by the Sox system, with cytochrome c551/c5 and HiPIP III directing electrons towards the Reaction Centre.
期刊介绍:
Environmental Microbiology provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following:
the structure, activities and communal behaviour of microbial communities
microbial community genetics and evolutionary processes
microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors
microbes in the tree of life, microbial diversification and evolution
population biology and clonal structure
microbial metabolic and structural diversity
microbial physiology, growth and survival
microbes and surfaces, adhesion and biofouling
responses to environmental signals and stress factors
modelling and theory development
pollution microbiology
extremophiles and life in extreme and unusual little-explored habitats
element cycles and biogeochemical processes, primary and secondary production
microbes in a changing world, microbially-influenced global changes
evolution and diversity of archaeal and bacterial viruses
new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens