CTRP9 通过增强自噬启动复合物的形成来增加脂肪分解,从而缓解饮食引起的肥胖。

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Nutritional Biochemistry Pub Date : 2024-06-19 DOI:10.1016/j.jnutbio.2024.109694
Shengyun Lei , Xuehui Li , Anju Zuo , Shiyan Ruan , Yuan Guo
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引用次数: 0

摘要

最近,越来越多的证据表明,肥胖已成为全球普遍存在的健康威胁。据报道,CTRP9 可改善高频分解膳食诱发的肥胖症。然而,CTRP9在肥胖症中发挥作用的分子机制仍未确定。在本研究中,我们报告了其在调控脂肪分解中的主要功能。首先,我们发现在肥胖小鼠的成熟脂肪细胞和白色脂肪组织中,CTRP9 的表达量减少。然后,我们发现过表达脂肪组织 CTRP9 可减轻饮食诱导的肥胖和脂肪细胞肥大,改善葡萄糖不耐受,提高能量消耗。此外,CTRP9 还能增加体外和体内的脂肪分解。此外,我们还发现 CTRP9 能增强脂肪细胞的自噬通量。有趣的是,通过 SiRNA 敲除 Beclin1 可消除 CTRP9 对脂肪分解的影响。CTRP9从机制上增强了SNX26的表达。我们证实,SNX26是ATG14L-Beclin1-VPS34复合物的一个组成部分,并能增强自噬启动复合物的组装。总之,我们的研究结果表明,CTRP9 可通过自噬启动复合物的形成促进脂肪分解,从而减轻饮食引起的肥胖。
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CTRP9 alleviates diet induced obesity through increasing lipolysis mediated by enhancing autophagy-initiation complex formation.

Recently, emerging evidence has suggested that obesity become a prevalent health threat worldwide. Reportedly, CTRP9 can ameliorate HFD induced obesity. However, the molecular mechanism underlying the role of CTRP9 in obesity remains elusive. In this study, we reported its major function in the regulation of lipolysis. First, we found that the expression of CTRP9 was decreased in mature adipocytes and white adipose tissue of obese mice. Then, we showed that overexpression adipose tissue CTRP9 alleviated diet-induced obesity and adipocytes hypertrophy, improved glucose intolerance and raised energy expenditure. Moreover, CTRP9 increased the lipolysis in vitro and vivo. Additionally, we determined that CTRP9 enhanced autophagy flux in adipocytes. Intriguingly, knock down Beclin1 by SiRNA abolished the effect of CTRP9 on lipolysis. Mechanically, CTRP9 enhanced the expression of SNX26. We demonstrated that SNX26 was a component of the ATG14L-Beclin1-VPS34 complex and enhanced the assembly of the autophagy-initiation complex. Collectively, our results suggested that CTRP9 alleviated diet induced obesity through enhancing lipolysis mediated by autophagy-initiation complex formation.

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来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
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