在进化早期分化的兰花亚科 Apostasioideae 中,Neuwiedia malipoensis 的碳获得方式和真菌关联。

IF 3.6 2区 生物学 Q1 PLANT SCIENCES Annals of botany Pub Date : 2024-08-22 DOI:10.1093/aob/mcae097
Franziska E Zahn, Hong Jiang, Yung-I Lee, Gerhard Gebauer
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引用次数: 0

摘要

背景和目的:最早分化的兰科植物Apostasioideae只有两个属:Apostasia 和 Neuwiedia。之前关于Apostasia nipponica的报道表明,它与外生菌根形成的Ceratobasidiaceae科植物有共生关系,并在成虫阶段部分利用真菌碳。然而,Neuwiedia 在整个发育过程中的营养策略仍未确定。为了进一步加深我们对 Apostasioideae 中菌根营养的了解,本研究以 Neuwiedia malipoensis 为研究对象,考察了该兰花物种在不同发育阶段的菌根关联和生理生态:方法:我们利用分子条形码鉴定了Neuwiedia malipoensis原球茎、叶状幼苗和成株阶段的主要菌根真菌。为了揭示麦饭石菌利用的营养资源,我们将不同发育阶段的稳定同位素天然丰度(δ13C、δ15N、δ2H、δ18O)与自养参照植物进行了比较:幼苗和成株主要与属于土拉菌科的腐生真菌有联系。虽然 13C 和 2H 稳定同位素数据显示幼苗具有部分绵状异养性,但目前还不清楚成虫中真菌碳供应的减少程度。我们的数据表明,在本体发育过程中,菌根伙伴发生了转变,N. malipoensis 对真菌氮和碳的依赖性降低:结论:N. malipoensis 和 A. nipponica 之间菌根伙伴的差异表明,在最早分化的兰科植物 Apostasioideae 中,存在着不同的资源获取策略和生境选择。尼波尼卡(A. nipponica)依赖于其外生菌根真菌伙伴的异养碳增量,因此依赖于森林生境,而麦饭石兰(N. malipoensis)则依赖于自身的光合碳增量作为成体,使其能够在分布广泛的生境中生长,如根瘤真菌生长的生境。
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Mode of carbon gain and fungal associations of Neuwiedia malipoensis within the evolutionarily early-diverging orchid subfamily Apostasioideae.

Background and aims: The earliest-diverging orchid lineage, Apostasioideae, consists only of two genera: Apostasia and Neuwiedia. Previous reports of Apostasia nipponica indicated a symbiotic association with an ectomycorrhiza-forming Ceratobasidiaceae clade and partial utilization of fungal carbon during the adult stage. However, the trophic strategy of Neuwiedia throughout its development remains unidentified. To further improve our understanding of mycoheterotrophy in the Apostasioideae, this study focused on Neuwiedia malipoensis examining both the mycorrhizal association and the physiological ecology of this orchid species across various development stages.

Methods: We identified the major mycorrhizal fungi of N. malipoensis protocorm, leafy seedling and adult stages using molecular barcoding. To reveal nutritional resources utilized by N. malipoensis, we compared stable isotope natural abundances (δ13C, δ15N, δ2H, δ18O) of different developmental stages with those of autotrophic reference plants.

Key results: Protocorms exhibited an association with saprotrophic Ceratobasidiaceae rather than ectomycorrhiza-forming Ceratobasidiaceae and the 13C signature was characteristic of their fully mycoheterotrophic nutrition. Seedlings and adults were predominantly associated with saprotrophic fungi belonging to the Tulasnellaceae. While 13C and 2H stable isotope data revealed partial mycoheterotrophy of seedlings, it is unclear to what extent the fungal carbon supply is reduced in adult N. malipoensis. However, the 15N enrichment of mature N. malipoensis suggests partially mycoheterotrophic nutrition. Our data indicated a transition in mycorrhizal partners during ontogenetic development with decreasing dependency of N. malipoensis on fungal nitrogen and carbon.

Conclusions: The divergence in mycorrhizal partners between N. malipoensis and A. nipponica indicates different resource acquisition strategies and allows various habitat options in the earliest-diverging orchid lineage, Apostasioideae. While A. nipponica relies on the heterotrophic carbon gain from its ectomycorrhizal fungal partner and thus on forest habitats, N. malipoensis rather relies on own photosynthetic carbon gain as an adult, allowing it to establish in habitats as widely distributed as those where Rhizoctonia fungi occur.

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来源期刊
Annals of botany
Annals of botany 生物-植物科学
CiteScore
7.90
自引率
4.80%
发文量
138
审稿时长
3 months
期刊介绍: Annals of Botany is an international plant science journal publishing novel and rigorous research in all areas of plant science. It is published monthly in both electronic and printed forms with at least two extra issues each year that focus on a particular theme in plant biology. The Journal is managed by the Annals of Botany Company, a not-for-profit educational charity established to promote plant science worldwide. The Journal publishes original research papers, invited and submitted review articles, ''Research in Context'' expanding on original work, ''Botanical Briefings'' as short overviews of important topics, and ''Viewpoints'' giving opinions. All papers in each issue are summarized briefly in Content Snapshots , there are topical news items in the Plant Cuttings section and Book Reviews . A rigorous review process ensures that readers are exposed to genuine and novel advances across a wide spectrum of botanical knowledge. All papers aim to advance knowledge and make a difference to our understanding of plant science.
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