Duen-Pang Kuo , Yung-Chieh Chen , Sho-Jen Cheng , Kevin Li-Chun Hsieh , Chen-Yin Ou , Yi-Tien Li , Cheng-Yu Chen
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MRI, including diffusion tensor imaging (DTI), T2-weighted imaging, perfusion-weighted imaging, and T1 mapping, was conducted at multiple time points for up to 5 days during the I/R phases. The spatiotemporal dynamics of blood–brain barrier (BBB) modifications were characterized through changes in T1 within the IP and IC regions and compared with mean diffusivity (MD), T2, and cerebral blood flow.</p></div><div><h3>Results</h3><p>During the I/R phases, the MD of the IC initially decreased, normalized after recanalization, decreased again at 24 h, and peaked on day 5. By contrast, the IP remained relatively stable. Both the IP and IC exhibited hyperperfusion, with the IP reaching its peak at 24 h, followed by resolution, whereas hyperperfusion was maintained in the IC until day 5. Despite hyperperfusion, the IP maintained an intact BBB, whereas the IC experienced persistent BBB leakage. 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引用次数: 0
摘要
简介尽管缺血再灌注(I/R)损伤在皮层和皮层下区域之间存在差异,但其对特定区域的影响仍不清楚。在这项研究中,我们使用了多种磁共振成像(MRI)技术来研究啮齿动物模型缺血半影(IP)和再灌注缺血核心(IC)内 I/R 损伤的时空动态,旨在通过阐明这些动态来改进治疗策略:共有 17 只 Sprague-Dawley 大鼠接受了 1 小时的瞬时大脑中动脉闭塞缝合模型。在I/R阶段的长达5天的时间内,在多个时间点进行核磁共振成像,包括弥散张量成像(DTI)、T2加权成像、灌注加权成像和T1图谱。研究人员通过IP和IC区域内T1的变化来描述血脑屏障(BBB)改变的时空动态,并将其与平均弥散度(MD)、T2和脑血流进行比较:结果:在I/R阶段,IC的MD最初下降,再通后恢复正常,24小时后再次下降,并在第5天达到峰值。相比之下,IP 保持相对稳定。IP 和 IC 都表现出高灌注,IP 在 24 小时达到峰值,随后缓解,而 IC 的高灌注一直维持到第 5 天。尽管存在高灌注,IP 仍保持完整的 BBB,而 IC 则出现持续的 BBB 渗漏。24小时后,集成电路的T2信号增加,与5天时出现BBB破坏的区域相对应:结论:高灌注和 BBB 损伤在 IP 和 IC 中具有不同的模式。定量 T1 映像可作为一种辅助工具,用于早期检测伴有 BBB 渗漏的恶性高灌注,有助于在再通后进行精确干预。这些发现强调了磁共振成像标记物在监测缺血特异性区域和定制治疗策略以改善患者预后方面的价值。
Ischemia-reperfusion injury in a salvaged penumbra: Longitudinal high-tesla perfusion magnetic resonance imaging in a rat model
Introduction
Although ischemia-reperfusion (I/R) injury varies between cortical and subcortical regions, its effects on specific regions remain unclear. In this study, we used various magnetic resonance imaging (MRI) techniques to examine the spatiotemporal dynamics of I/R injury within the salvaged ischemic penumbra (IP) and reperfused ischemic core (IC) of a rodent model, with the aim of enhancing therapeutic strategies by elucidating these dynamics.
Materials and methods
A total of 17 Sprague–Dawley rats were subjected to 1 h of transient middle cerebral artery occlusion with a suture model. MRI, including diffusion tensor imaging (DTI), T2-weighted imaging, perfusion-weighted imaging, and T1 mapping, was conducted at multiple time points for up to 5 days during the I/R phases. The spatiotemporal dynamics of blood–brain barrier (BBB) modifications were characterized through changes in T1 within the IP and IC regions and compared with mean diffusivity (MD), T2, and cerebral blood flow.
Results
During the I/R phases, the MD of the IC initially decreased, normalized after recanalization, decreased again at 24 h, and peaked on day 5. By contrast, the IP remained relatively stable. Both the IP and IC exhibited hyperperfusion, with the IP reaching its peak at 24 h, followed by resolution, whereas hyperperfusion was maintained in the IC until day 5. Despite hyperperfusion, the IP maintained an intact BBB, whereas the IC experienced persistent BBB leakage. At 24 h, the IC exhibited an increase in the T2 signal, corresponding to regions exhibiting BBB disruption at 5 days.
Conclusions
Hyperperfusion and BBB impairment have distinct patterns in the IP and IC. Quantitative T1 mapping may serve as a supplementary tool for the early detection of malignant hyperemia accompanied by BBB leakage, aiding in precise interventions after recanalization. These findings underscore the value of MRI markers in monitoring ischemia-specific regions and customizing therapeutic strategies to improve patient outcomes.
期刊介绍:
Magnetic Resonance Imaging (MRI) is the first international multidisciplinary journal encompassing physical, life, and clinical science investigations as they relate to the development and use of magnetic resonance imaging. MRI is dedicated to both basic research, technological innovation and applications, providing a single forum for communication among radiologists, physicists, chemists, biochemists, biologists, engineers, internists, pathologists, physiologists, computer scientists, and mathematicians.