{"title":"2-乙基己醇--一种由 Stenotrophomonas sp.","authors":"Yu Zhang, Zeyuan Li, Shuxin Wei, Changsheng Xu, Minhua Chen, Jierong Sang, Yiru Han, Huang Yan, Zhoukun Li, Zhongli Cui and Xianfeng Ye*, ","doi":"10.1021/acs.jafc.3c09851","DOIUrl":null,"url":null,"abstract":"<p >Researchers often consider microorganisms from <i>Stenotrophomonas</i> sp. to be beneficial for plants. In this study, the biocidal effects and action mechanisms of volatile organic compounds (VOCs) produced by <i>Stenotrophomonas</i> sp. NAU1697 were investigated. The mycelial growth and spore germination of <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (FOC), which is a pathogen responsible for cucumber wilt disease, were significantly inhibited by VOCs emitted from NAU1697. Among the VOCs, 33 were identified, 11 of which were investigated for their antifungal properties. Among the tested compounds, 2-ethylhexanol exhibited the highest antifungal activity toward FOC, with a minimum inhibitory volume (MIV) of 3.0 μL/plate (equal to 35.7 mg/L). Damage to the hyphal cell wall and cell membrane integrity caused a decrease in the ergosterol content and a burst of reactive oxygen species (ROS) after 2-ethylhexanol treatment. DNA damage, which is indicative of apoptosis-like cell death, was monitored in 2-ethylhexanol-treated FOC cells by using micro-FTIR analysis. Furthermore, the activities of mitochondrial dehydrogenases and mitochondrial respiratory chain complex III in 2-ethylhexanol-treated FOC cells were significantly decreased. The transcription levels of genes associated with redox reactions and the cell wall integrity (CWI) pathway were significantly upregulated, thus indicating that stress was caused by 2-ethylhexanol. The findings of this research provide a new avenue for the sustainable management of soil-borne plant fungal diseases.</p>","PeriodicalId":41,"journal":{"name":"Journal of Agricultural and Food Chemistry","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antifungal Activity and Mechanisms of 2-Ethylhexanol, a Volatile Organic Compound Produced by Stenotrophomonas sp. NAU1697, against Fusarium oxysporum f. sp. cucumerinum\",\"authors\":\"Yu Zhang, Zeyuan Li, Shuxin Wei, Changsheng Xu, Minhua Chen, Jierong Sang, Yiru Han, Huang Yan, Zhoukun Li, Zhongli Cui and Xianfeng Ye*, \",\"doi\":\"10.1021/acs.jafc.3c09851\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Researchers often consider microorganisms from <i>Stenotrophomonas</i> sp. to be beneficial for plants. In this study, the biocidal effects and action mechanisms of volatile organic compounds (VOCs) produced by <i>Stenotrophomonas</i> sp. NAU1697 were investigated. The mycelial growth and spore germination of <i>Fusarium oxysporum</i> f. sp. <i>cucumerinum</i> (FOC), which is a pathogen responsible for cucumber wilt disease, were significantly inhibited by VOCs emitted from NAU1697. Among the VOCs, 33 were identified, 11 of which were investigated for their antifungal properties. Among the tested compounds, 2-ethylhexanol exhibited the highest antifungal activity toward FOC, with a minimum inhibitory volume (MIV) of 3.0 μL/plate (equal to 35.7 mg/L). Damage to the hyphal cell wall and cell membrane integrity caused a decrease in the ergosterol content and a burst of reactive oxygen species (ROS) after 2-ethylhexanol treatment. DNA damage, which is indicative of apoptosis-like cell death, was monitored in 2-ethylhexanol-treated FOC cells by using micro-FTIR analysis. Furthermore, the activities of mitochondrial dehydrogenases and mitochondrial respiratory chain complex III in 2-ethylhexanol-treated FOC cells were significantly decreased. The transcription levels of genes associated with redox reactions and the cell wall integrity (CWI) pathway were significantly upregulated, thus indicating that stress was caused by 2-ethylhexanol. 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引用次数: 0
摘要
研究人员通常认为,来自 Stenotrophomonas sp.的微生物对植物有益。在本研究中,研究了由 Stenotrophomonas sp. NAU1697 产生的挥发性有机化合物(VOCs)的杀菌效果和作用机制。NAU1697释放的挥发性有机化合物显著抑制了黄瓜枯萎病病原菌Fusarium oxysporum f. sp. cucumerinum(FOC)的菌丝生长和孢子萌发。在确定的33种挥发性有机化合物中,对其中11种进行了抗真菌特性研究。在测试的化合物中,2-乙基己醇对FOC的抗真菌活性最高,最小抑制量(MIV)为3.0 μL/平板(相当于35.7 mg/L)。2-ethylhexanol 处理后,对真菌细胞壁和细胞膜完整性的破坏导致麦角固醇含量下降和活性氧(ROS)爆发。在 2-ethylhexanol 处理的 FOC 细胞中,利用显微傅立叶变换红外光谱分析监测了 DNA 损伤,这是细胞凋亡的标志。此外,2-乙基己醇处理的 FOC 细胞中线粒体脱氢酶和线粒体呼吸链复合物 III 的活性显著降低。与氧化还原反应和细胞壁完整性(CWI)通路相关的基因转录水平明显上调,从而表明 2-乙基己醇导致了应激反应。这项研究成果为土传植物真菌病害的可持续管理提供了一条新途径。
Antifungal Activity and Mechanisms of 2-Ethylhexanol, a Volatile Organic Compound Produced by Stenotrophomonas sp. NAU1697, against Fusarium oxysporum f. sp. cucumerinum
Researchers often consider microorganisms from Stenotrophomonas sp. to be beneficial for plants. In this study, the biocidal effects and action mechanisms of volatile organic compounds (VOCs) produced by Stenotrophomonas sp. NAU1697 were investigated. The mycelial growth and spore germination of Fusarium oxysporum f. sp. cucumerinum (FOC), which is a pathogen responsible for cucumber wilt disease, were significantly inhibited by VOCs emitted from NAU1697. Among the VOCs, 33 were identified, 11 of which were investigated for their antifungal properties. Among the tested compounds, 2-ethylhexanol exhibited the highest antifungal activity toward FOC, with a minimum inhibitory volume (MIV) of 3.0 μL/plate (equal to 35.7 mg/L). Damage to the hyphal cell wall and cell membrane integrity caused a decrease in the ergosterol content and a burst of reactive oxygen species (ROS) after 2-ethylhexanol treatment. DNA damage, which is indicative of apoptosis-like cell death, was monitored in 2-ethylhexanol-treated FOC cells by using micro-FTIR analysis. Furthermore, the activities of mitochondrial dehydrogenases and mitochondrial respiratory chain complex III in 2-ethylhexanol-treated FOC cells were significantly decreased. The transcription levels of genes associated with redox reactions and the cell wall integrity (CWI) pathway were significantly upregulated, thus indicating that stress was caused by 2-ethylhexanol. The findings of this research provide a new avenue for the sustainable management of soil-borne plant fungal diseases.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.