甲基转移酶 METTL15 在线粒体核糖体生物发生过程中的作用离不开其催化活性。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-06-24 DOI:10.1080/15476286.2024.2369374
Christian D Mutti, Lindsey Van Haute, Michal Minczuk
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引用次数: 0

摘要

核糖体是由蛋白质和 RNA 组成的大分子复合体,其生物生成需要大量的因子和转录后修饰。在人类线粒体中,核糖体 RNA 经过十个位点的转录后修饰。N4-甲基胞嘧啶(m4C)甲基转移酶 METTL15 在 C1486 位修饰小亚基的 12S rRNA。如本文和之前的研究所示,该酶对线粒体蛋白质合成和 mitoribosome 小亚基的组装至关重要。在这里,我们证明了小亚基的生物发生不需要 m4C 修饰,这表明 METTL15 蛋白本身的类似伴侣的活性是 mitoribosome 生物发生的重要组成部分。
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The catalytic activity of methyltransferase METTL15 is dispensable for its role in mitochondrial ribosome biogenesis.

Ribosomes are large macromolecular complexes composed of both proteins and RNA, that require a plethora of factors and post-transcriptional modifications for their biogenesis. In human mitochondria, the ribosomal RNA is post-transcriptionally modified at ten sites. The N4-methylcytidine (m4C) methyltransferase, METTL15, modifies the 12S rRNA of the small subunit at position C1486. The enzyme is essential for mitochondrial protein synthesis and assembly of the mitoribosome small subunit, as shown here and by previous studies. Here, we demonstrate that the m4C modification is not required for small subunit biogenesis, indicating that the chaperone-like activity of the METTL15 protein itself is an essential component for mitoribosome biogenesis.

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来源期刊
RNA Biology
RNA Biology 生物-生化与分子生物学
CiteScore
8.60
自引率
0.00%
发文量
82
审稿时长
1 months
期刊介绍: RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy
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