源内碎片对代谢和化学质谱数据解读的隐性影响

IF 18.9 1区医学 Q1 ENDOCRINOLOGY & METABOLISM Nature metabolism Pub Date : 2024-06-25 DOI:10.1038/s42255-024-01076-x

Martin Giera, Aries Aisporna, Winnie Uritboonthai, Gary Siuzdak

引用次数: 0

摘要

质谱法，特别是液相色谱-串联质谱法（LC-MS/MS），是代谢组学、制药和临床分析的前沿技术，在检测生物样本中的代谢物和其他小分子方面具有高灵敏度和特异性。然而，这项强大的技术却揭示了大量令人费解的未识别光谱特征，被称为 "黑暗代谢组 "1,2，与已知的代谢多样性形成了鲜明对比。人类已知拥有约 20,000 个蛋白质编码基因3,4 ，其中只有一小部分表达酶，而 LC-MS/MS 所显示的假定化学多样性表明，还有数十万到数百万个代谢物有待鉴定2,5。生物学的核心教条描述了从基因组到转录组、蛋白质组、代谢组等不同层次之间的关系和信息流，其中基因组是任何生命系统在诞生之初就烙下的主代码6。连接前三层的代码相当简单，三个核苷酸编码一个氨基酸。然而，代谢组的这种加密方式尚未被揭示。这一事实，再加上大量未标注的 LC-MS/MS 数据，是代谢组（生物系统中的全部小分子）尚无法定义的两个主要原因。根据目前的估计，只有不到 2% 的 LC-MS/MS 图谱可以进行标注，这就意味着可能存在大量未知化合物2。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature metabolism ENDOCRINOLOGY & METABOLISM-

CiteScore

27.50

自引率

2.40%

发文量

170

期刊介绍： Nature Metabolism is a peer-reviewed scientific journal that covers a broad range of topics in metabolism research. It aims to advance the understanding of metabolic and homeostatic processes at a cellular and physiological level. The journal publishes research from various fields, including fundamental cell biology, basic biomedical and translational research, and integrative physiology. It focuses on how cellular metabolism affects cellular function, the physiology and homeostasis of organs and tissues, and the regulation of organismal energy homeostasis. It also investigates the molecular pathophysiology of metabolic diseases such as diabetes and obesity, as well as their treatment. Nature Metabolism follows the standards of other Nature-branded journals, with a dedicated team of professional editors, rigorous peer-review process, high standards of copy-editing and production, swift publication, and editorial independence. The journal has a high impact factor, has a certain influence in the international area, and is deeply concerned and cited by the majority of scholars.

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