Renata Blocher, Ying Liu, Tayler Patrick, Irina A Polejaeva
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引用次数: 0
摘要
牛体外卵母细胞成熟(IVM)是为后续辅助生殖技术获取卵母细胞的一种简便方法,但与体内成熟相比效率较低。在多个物种中,补充成纤维细胞生长因子 2 (FGF2)、白血病抑制因子 (LIF) 和胰岛素样生长因子 1 (IGF1) 或 FLI 这三种细胞因子可提高卵母细胞成熟度和胚胎发育,但尚未有研究探讨补充 FLI IVM 所导致的卵母细胞差异。本研究旨在评估优质卵母细胞中重要的核和胞质成熟事件。补充 FLI 的卵母细胞在 IVM 后 GV 下降(3.0% vs. 13.7%,p < 0.01),端期 I 发生率增加(34.6% vs. 17.6%,p < 0.05),正常减数分裂轴增加(68.8% vs. 50.0%,p < 0.001),核成熟率增加(75.1% vs. 66.8%,p < 0.001)。此外,在二分裂期卵母细胞中,经 FLI 处理的卵母细胞中线粒体呈弥漫分布的比例较高(87.7% 对 77.5%,p<0.05),线粒体呈皮层分布的比例较低(11.6% 对 17.4%,p<0.05)。此外,补充 FLI 的卵母细胞具有更多的 I 型皮质颗粒(21.3% 对 14.4%,p < 0.05)。这些数据表明,在牛体外成熟培养基中补充 FLI 可协调细胞核和细胞质的成熟,从而产生更高质量的卵母细胞。
Cytokine-Supplemented Maturation Medium Enhances Cytoplasmic and Nuclear Maturation in Bovine Oocytes.
Bovine in vitro oocyte maturation (IVM) is an easy way to obtain oocytes for subsequent assisted reproductive techniques but is inefficient compared to in vivo maturation. Supplementation of three cytokines, fibroblast growth factor 2 (FGF2), leukemia inhibitory factor (LIF), and insulin-like growth factor 1 (IGF1), or FLI, has increased oocyte maturation and embryo development in multiple species, but studies have not explored the oocyte differences caused by FLI IVM supplementation. This study aimed to assess important nuclear and cytoplasmic maturation events in high-quality oocytes. FLI-supplemented oocytes had a decreased GV (3.0% vs. 13.7%, p < 0.01) and increased telophase I incidence (34.6% vs. 17.6%, p < 0.05) after IVM, increased normal meiotic spindles (68.8% vs. 50.0%, p < 0.001), and an increased nuclear maturation rate (75.1% vs. 66.8%, p < 0.001). Moreover, in metaphase II oocytes, the percentage of FLI-treated oocytes with a diffuse mitochondrial distribution was higher (87.7% vs. 77.5%, p < 0.05) and with a cortical mitochondrial distribution was lower (11.6% vs. 17.4%, p < 0.05). Additionally, FLI-supplemented oocytes had more pattern I cortical granules (21.3% vs. 14.4%, p < 0.05). These data suggest that FLI supplementation in bovine in vitro maturation medium coordinates nuclear and cytoplasmic maturation to produce higher-quality oocytes.
AnimalsAgricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍:
Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).