新型嗜热芽孢杆菌 ASFS1 生物合成的氧化铁纳米粒子的纯化、表征和抗癌活性评估

IF 3.5 4区 生物学 Q2 MICROBIOLOGY Journal of Basic Microbiology Pub Date : 2024-06-23 DOI:10.1002/jobm.202400153
Naghmeh Satarzadeh, Mojtaba Shakibaie, Hamid Forootanfar, Bagher Amirheidari
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引用次数: 0

摘要

磁性纳米粒子(MNPs),特别是氧化铁纳米粒子(IONPs),是一组令人着迷的纳米粒子,由于其超顺磁性能、生物可降解性和生物兼容性,在生物医学应用方面受到了广泛的研究。研究人员分离并鉴定了一种新型革兰氏阳性中度嗜热细菌菌株,即茶碱芽孢杆菌(Bacillus tequilensis ASFS.1)。该菌株能够产生超顺磁性的 Fe3O4 纳米粒子,并表现出磁导行为。研究人员对该菌株在静态和动态环境下的游动行为进行了调查,发现其行为与趋磁细菌的磁导行为非常相似。这项研究首次报道了一种芽孢杆菌科细菌具有在细胞内生物合成 IONPs 的潜力。本研究首次设计了一种磁性和可重复的方法来分离 MNPs。此外,还利用紫外可见分光光度计、傅立叶变换红外光谱仪、振动样品磁力计、场发射扫描电子显微镜(FESEM)、X 射线衍射和热重力分析来表征生物制造的磁铁矿纳米粒子。通过 FESEM 成像分析生物源 MNPs 的粒度分布模式,发现其粒度范围为 10-100 nm,其中 10-40 nm 的 MNPs 最常见。VSM 分析表明,生物源 MNPs 具有超顺磁性,饱和磁化值高达 184 emu/g。用生物源 MNPs 处理 3T3、U87、A549、MCF-7 和 HT-29 细胞株 24 小时后,测得的 IC50 值分别为 339、641、582、149 和 184 μg mL-1。本研究介绍了新型菌株 ASFS.1借助其磁铁矿纳米颗粒的磁致伸缩能力,并为其 MNPs 的分离、表征和体外细胞毒性提供了信息。这些 MNPs 在生物医学应用方面展现出了巨大的潜力,但显然还有待进一步研究。
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Purification, Characterization, and Assessment of Anticancer Activity of Iron Oxide Nanoparticles Biosynthesized by Novel Thermophilic Bacillus tequilensis ASFS1‏

Magnetic nanoparticles (MNPs), particularly iron oxide nanoparticles (IONPs), are a fascinating group of nanoparticles that have been considerably investigated for biomedical applications because of their superparamagnetic properties, biodegradable nature, and biocompatibility. A novel Gram-positive moderately thermophilic bacterial strain, namely Bacillus tequilensis ASFS.1, was isolated and identified. This strain is capable of producing superparamagnetic Fe3O4 nanoparticles and exhibiting magnetotaxis behavior. This strain swimming behavior was investigated under static and dynamic environments, where it behaved very much similar to the magnetotaxis in magnetotactic bacteria. This study is the first report of a bacterium from the Bacillaceae family that has the potential to intracellular biosynthesis of IONPs. MNPs were separated by a magnetic and reproducible method which was designed for the first time for this study. In addition, UV-visible spectrophotometer, Fourier-transform infrared spectroscopy, vibrating sample magnetometer, field emission scanning electron microscopy (FESEM), X-ray diffraction, and thermal gravimetric analysis were utilized to characterize the bio-fabricated magnetite nanoparticles. Analysis of the particle size distribution pattern of the biogenic MNPs by FESEM imaging revealed the size range of 10–100 nm with the size range of 10–40 nm MNPs being the most frequent particles. VSM analysis demonstrated that biogenic MNPs displayed superparamagnetic properties with a high saturation magnetization value of 184 emu/g. After 24 h treatment of 3T3, U87, A549, MCF-7, and HT-29 cell lines with the biogenic MNPs, IC50 values were measured to be 339, 641, 582, 149, and 184 μg mL−1, respectively. This study presents the novel strain ASFS.1 capable of magnetotaxis by the aid of its magnetite nanoparticles and paving information on isolation, characterization, and in vitro cytotoxicity of its MNPs. The MNPs showed promising potential for biomedical applications, obviously subject to additional studies.

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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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