用激光和扫描电子显微镜评估牙结石残留微区

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of periodontology Pub Date : 2024-06-28 DOI:10.1002/jper.24-0191
Stephen K. Harrel, Charles M. Cobb, Martha E. Nunn, Donggao Zhao
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引用次数: 0

摘要

背景最近的研究表明,残留牙结石的微小结晶颗粒在牙周炎的发病机制中扮演着重要角色。这项体内外研究的目的是比较单纯洗牙和根面平整(SRP)与结合 24% 乙二胺四乙酸(EDTA)凝胶的 SRP 在去除拔牙结石方面的效果,并确定使用 EDTA 的最佳时间长度。在每颗牙齿的根部表面制备一个直径为 4 毫米的部位,然后进行 SRP。使用白光(WL)和激光荧光(LF)以 40 倍放大率拍摄显微照片。显微照片使用 ImageJ 进行分析。结果SRP后残留结石的平均面积为45%-53%(45.6% ± 19.6% WL,53.8% ± 19.7% LF)。SRP 后用 EDTA 烧灼一分钟,结石仅减少了 14%-18%(13.9% ± 12.5% LF,18.2% ± 11.1% WL)。使用 EDTA 超过 1 分钟后,牙结石没有进一步去除。扫描电子显微镜显示,剩余结石的表面因使用 EDTA 烧灼而发生了改变。单独使用 SRP 清除了牙根表面 60% 的牙结石。辅助使用 24% EDTA 凝胶烧灼牙根表面,可去除 SRP 后残留的大部分牙结石。EDTA 烧灼后残留的牙结石在形态上有明显的改变。
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Laser and scanning electron microscopy evaluation of residual microislands of calculus
BackgroundRecent studies suggest a role for microscopic crystalline particles of residual dental calculus in the pathogenesis of periodontitis. The purpose of this ex vivo study was to compare the effectiveness of scaling and root planing (SRP) alone versus SRP combined with 24% ethylenediamine‐tetra acetic acid (EDTA) gel in removing calculus from extracted teeth and to determine the optimal length of time for application of the EDTA.MethodsSpecimens consisted of 32 extracted teeth with heavy root calculus. A 4‐mm diameter site was prepared on the root surface of each tooth which then underwent SRP. EDTA was applied to four timed groups: 30 s; 60 s; 120 s; and 180 s. Photomicrographs were taken at 40× magnification using white light (WL) and laser fluorescence (LF). Photomicrographs were analyzed using ImageJ. Specimens were also evaluated with scanning electron microscopy (SEM).ResultsThe mean area of residual calculus after SRP was 45%–53% (45.6% ± 19.6% WL, 53.8% ± 19.7% LF). Burnishing with EDTA for one minute following SRP reduced calculus to only 14%–18% (13.9% ± 12.5% LF, 18.2% ± 11.1% WL). Use of EDTA for greater than 1 min showed no further calculus removal. SEM revealed the surface of remaining calculus was altered by burnishing with EDTA.ConclusionSRP alone or SRP + 24% EDTA gel failed to remove all calculus. SRP alone removed >60% of calculus from root surfaces. Adjunctive use of 24% EDTA gel burnished on the root surface removed most of the calculus residual after SRP. Calculus remaining after EDTA burnishing exhibited a significantly altered morphologic appearance.
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
期刊最新文献
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