Alan Bao, Xian Xia, Hao Wang, Qin Li, Chunmei Chen*, Yonghui Zhang* and Hucheng Zhu*,
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引用次数: 0
摘要
通过核磁共振、HRESIMS、Mo2(OAc)4 诱导的圆二色性(ICD)和电子圆二色性(ECD)计算,从真菌哈茨真菌(Trichoderma harzianum)中分离出了一种新的 fusicoccane 二萜类化合物--哈茨真菌 A(1)、两种新的哈茨二萜类化合物--哈茨酮 G 和 H(2 和 3)、一种修正的哈茨二萜类化合物(4)以及两种已知的二萜类化合物(5 和 6)。值得注意的是,化合物 1 是首次从哈茨蘑菇中提取的呋喃杂环庚烷类二萜化合物。通过仔细的光谱分析,呋喃哈嗪酮 B 的结构被修正为 4。此外,化合物 2 和 5 还能抑制食源性细菌病原体蜡样芽孢杆菌的整体生长。化合物 4 对脂多糖(LPS)处理的 RAW 264.7 细胞中 NO 的生成有适度的抑制作用。本研究的发现不仅扩大了从哈茨藻中分离出的二萜化合物的结构种类,还为开发哈茨二萜化合物作为抗食源性病原体制剂奠定了坚实的基础。
Diterpenoids with Antibacterial Activities from the Fungus Trichoderma harzianum
A new fusicoccane diterpenoid, harziaderma A (1), two novel harziane diterpenoids, harzianones G and H (2 and 3), one revised harziane diterpenoid (4), and two known diterpenoids (5 and 6) were isolated from the fungus Trichoderma harzianum and established via NMR, HRESIMS, Mo2(OAc)4-induced circular dichroism (ICD) and electronic circular dichroism (ECD) calculations. It is worth noting that compound 1 represents the first instance of a fusicoccane-type diterpenoid derived from T. harzianum. The structure of furanharzianone B was revised to 4 via careful spectroscopic analyses. Additionally, compounds 2 and 5 could suppress the overall growth of the foodborne bacterial pathogen Bacillus cereus. Compound 4 showed a moderate suppressive impact on NO generation in lipopolysaccharide (LPS)-treated RAW 264.7 cells. The discoveries from the current study not only expanded the structural variety of diterpenoids isolated from T. harzianum but also laid a robust foundation for the development of harziane diterpenoids as anti-foodborne pathogen agents.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.