Yu Yan , Zhenping Liu , Wenbin Pang , Shijian Huang , Mengxin Deng , Jiyuan Yao , Qiuju Huang , Mingliang Jin , Lingling Shui
{"title":"通过具有有序反波谱结构的原位自组装碳纳米管实现用于癌症多重生物标记诊断的集成生物传感器阵列。","authors":"Yu Yan , Zhenping Liu , Wenbin Pang , Shijian Huang , Mengxin Deng , Jiyuan Yao , Qiuju Huang , Mingliang Jin , Lingling Shui","doi":"10.1016/j.bios.2024.116528","DOIUrl":null,"url":null,"abstract":"<div><p>To enhance the precision and reliability of early disease detection, especially in malignancies, an exhaustive investigation of multi-target biomarkers is essential. In this study, an advanced integrated electrochemical biosensor array that demonstrates exceptional performance was constructed. This biosensor was developed through a controllable porous-size mechanism and <em>in-situ</em> modification of carbon nanotubes (CNTs) to quantify multiplex biomarkers—specifically, C-reaction protein (CRP), carbohydrate antigen 125 (CA125), and carcinoembryonic antigen (CEA)—in human serum plasma. The fabrication process involved creating a highly ordered three-dimensional inverse-opal structure with the CNTs (<em>p</em>CNTs) modifier through microdroplet-based microfluidics, confined spatial self-assembly of nanoparticles, and chemical wet-etching. This innovative approach allowed for direct <em>in-situ</em> modification of nanomaterial onto the surface of electrode array, eliminating secondary transfer and providing exceptional control over structure and stability. The outstanding electrochemical performance was achieved through the synergistic effect of the <em>p</em>CNTs nanomaterial, aptamer, and horseradish peroxidase-labeled (HRP-) antibody. Additionally, the integrated biosensor array platform comprised multiple individually addressable electrode units (<em>n</em> = 11), enabling simultaneous multi-parallel/target testing, thereby ensuring accuracy and high throughput. Crucially, this integrated biosensor array accurately quantified multiplex biomarkers in human serum, yielding results comparable to commercial methods. 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引用次数: 0
摘要
为了提高早期疾病(尤其是恶性肿瘤)检测的准确性和可靠性,必须对多目标生物标记物进行详尽的研究。本研究构建了一种性能卓越的先进集成电化学生物传感器阵列。这种生物传感器是通过一种可控多孔尺寸机制和碳纳米管(CNTs)原位修饰技术开发出来的,用于量化人血清血浆中的多重生物标记物,特别是 C 反应蛋白(CRP)、碳水化合物抗原 125(CA125)和癌胚抗原(CEA)。制造过程包括通过基于微液滴的微流体技术、纳米颗粒的封闭空间自组装和化学湿蚀刻技术,用碳纳米管(pCNTs)改性剂创建高度有序的三维反波醛结构。这种创新方法可将纳米材料直接原位修饰到电极阵列表面,消除了二次转移,并提供了对结构和稳定性的出色控制。pCNTs 纳米材料、aptamer 和辣根过氧化物酶标记(HRP)抗体的协同作用实现了出色的电化学性能。此外,集成生物传感器阵列平台由多个可单独寻址的电极单元(n = 11)组成,可同时进行多平行/目标测试,从而确保了准确性和高通量。最重要的是,该集成生物传感器阵列能准确量化人血清中的多重生物标志物,其结果可与商业方法相媲美。这项集成技术为早期疾病诊断和生物分析中的床旁检测(POCT)带来了希望。
Integrated biosensor array for multiplex biomarkers cancer diagnosis via in-situ self-assembly carbon nanotubes with an ordered inverse-opal structure
To enhance the precision and reliability of early disease detection, especially in malignancies, an exhaustive investigation of multi-target biomarkers is essential. In this study, an advanced integrated electrochemical biosensor array that demonstrates exceptional performance was constructed. This biosensor was developed through a controllable porous-size mechanism and in-situ modification of carbon nanotubes (CNTs) to quantify multiplex biomarkers—specifically, C-reaction protein (CRP), carbohydrate antigen 125 (CA125), and carcinoembryonic antigen (CEA)—in human serum plasma. The fabrication process involved creating a highly ordered three-dimensional inverse-opal structure with the CNTs (pCNTs) modifier through microdroplet-based microfluidics, confined spatial self-assembly of nanoparticles, and chemical wet-etching. This innovative approach allowed for direct in-situ modification of nanomaterial onto the surface of electrode array, eliminating secondary transfer and providing exceptional control over structure and stability. The outstanding electrochemical performance was achieved through the synergistic effect of the pCNTs nanomaterial, aptamer, and horseradish peroxidase-labeled (HRP-) antibody. Additionally, the integrated biosensor array platform comprised multiple individually addressable electrode units (n = 11), enabling simultaneous multi-parallel/target testing, thereby ensuring accuracy and high throughput. Crucially, this integrated biosensor array accurately quantified multiplex biomarkers in human serum, yielding results comparable to commercial methods. This integrated technology holds promise for point-of-care testing (POCT) in early disease diagnosis and biological analysis.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.