{"title":"应用抗血管内皮生长因子抗体恢复 2 型糖尿病小鼠模型的唾液分泌功能。","authors":"","doi":"10.1016/j.job.2024.06.011","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p><span><span><span>Xerostomia<span><span>, a common complication of type 2 diabetes, leads to an increased risk of caries, </span>dysphagia, and </span></span>dysgeusia. Although anti-vascular endothelial growth factor (VEGF) antibodies, such as </span>ranibizumab (RBZ), have been used to treat </span>diabetic retinopathy<span>, their effects on the salivary glands<span> are unknown. This study evaluated the effects of RBZ on salivary glands<span> to reduce inflammation and restore salivary function in a mouse model of type 2 diabetes.</span></span></span></p></div><div><h3>Methods</h3><p>Male KK-A<sup>y</sup><span> mice with type 2 diabetes (10–12 weeks old) were used. The diabetes mellitus (DM) group received phosphate-buffered saline, while the DM + RBZ group received an intraperitoneal administration of RBZ (100 μg/kg) 24 h before the experiment.</span></p></div><div><h3>Results</h3><p><span><span>Ex vivo</span></span><span><span> perfusion experiments showed a substantial increase in salivary secretion<span> from the submandibular gland<span><span><span> (SMG) in the DM + RBZ group. In addition, the mRNA expression levels of TNF-α and IL-1β were considerably lower in this group. In contrast, those of </span>aquaporin 5 were substantially higher in the DM + RBZ group, as revealed by </span>quantitative reverse transcription PCR. Furthermore, the number of </span></span></span>lymphocyte infiltration spots in the SMG was notably lower in the DM + RBZ group. Finally, intracellular Ca</span><sup>2+</sup><span> signaling in acinar cells was considerably higher in the DM + RBZ group than that in the DM group.</span></p></div><div><h3>Conclusion</h3><p>Treating a type 2 diabetic mouse model with RBZ restored salivary secretion through its anti-inflammatory effects.</p></div>","PeriodicalId":45851,"journal":{"name":"Journal of Oral Biosciences","volume":"66 3","pages":"Pages 619-627"},"PeriodicalIF":2.6000,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of anti-vascular endothelial growth factor antibody restores the function of saliva secretion in a type 2 diabetes mouse model\",\"authors\":\"\",\"doi\":\"10.1016/j.job.2024.06.011\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p><span><span><span>Xerostomia<span><span>, a common complication of type 2 diabetes, leads to an increased risk of caries, </span>dysphagia, and </span></span>dysgeusia. Although anti-vascular endothelial growth factor (VEGF) antibodies, such as </span>ranibizumab (RBZ), have been used to treat </span>diabetic retinopathy<span>, their effects on the salivary glands<span> are unknown. This study evaluated the effects of RBZ on salivary glands<span> to reduce inflammation and restore salivary function in a mouse model of type 2 diabetes.</span></span></span></p></div><div><h3>Methods</h3><p>Male KK-A<sup>y</sup><span> mice with type 2 diabetes (10–12 weeks old) were used. The diabetes mellitus (DM) group received phosphate-buffered saline, while the DM + RBZ group received an intraperitoneal administration of RBZ (100 μg/kg) 24 h before the experiment.</span></p></div><div><h3>Results</h3><p><span><span>Ex vivo</span></span><span><span> perfusion experiments showed a substantial increase in salivary secretion<span> from the submandibular gland<span><span><span> (SMG) in the DM + RBZ group. In addition, the mRNA expression levels of TNF-α and IL-1β were considerably lower in this group. In contrast, those of </span>aquaporin 5 were substantially higher in the DM + RBZ group, as revealed by </span>quantitative reverse transcription PCR. Furthermore, the number of </span></span></span>lymphocyte infiltration spots in the SMG was notably lower in the DM + RBZ group. Finally, intracellular Ca</span><sup>2+</sup><span> signaling in acinar cells was considerably higher in the DM + RBZ group than that in the DM group.</span></p></div><div><h3>Conclusion</h3><p>Treating a type 2 diabetic mouse model with RBZ restored salivary secretion through its anti-inflammatory effects.</p></div>\",\"PeriodicalId\":45851,\"journal\":{\"name\":\"Journal of Oral Biosciences\",\"volume\":\"66 3\",\"pages\":\"Pages 619-627\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-06-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral Biosciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1349007924001488\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral Biosciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1349007924001488","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Application of anti-vascular endothelial growth factor antibody restores the function of saliva secretion in a type 2 diabetes mouse model
Objectives
Xerostomia, a common complication of type 2 diabetes, leads to an increased risk of caries, dysphagia, and dysgeusia. Although anti-vascular endothelial growth factor (VEGF) antibodies, such as ranibizumab (RBZ), have been used to treat diabetic retinopathy, their effects on the salivary glands are unknown. This study evaluated the effects of RBZ on salivary glands to reduce inflammation and restore salivary function in a mouse model of type 2 diabetes.
Methods
Male KK-Ay mice with type 2 diabetes (10–12 weeks old) were used. The diabetes mellitus (DM) group received phosphate-buffered saline, while the DM + RBZ group received an intraperitoneal administration of RBZ (100 μg/kg) 24 h before the experiment.
Results
Ex vivo perfusion experiments showed a substantial increase in salivary secretion from the submandibular gland (SMG) in the DM + RBZ group. In addition, the mRNA expression levels of TNF-α and IL-1β were considerably lower in this group. In contrast, those of aquaporin 5 were substantially higher in the DM + RBZ group, as revealed by quantitative reverse transcription PCR. Furthermore, the number of lymphocyte infiltration spots in the SMG was notably lower in the DM + RBZ group. Finally, intracellular Ca2+ signaling in acinar cells was considerably higher in the DM + RBZ group than that in the DM group.
Conclusion
Treating a type 2 diabetic mouse model with RBZ restored salivary secretion through its anti-inflammatory effects.