从巴西白耳负鼠 Didelphis albiventris 中分离出一种新型复发性热病波氏杆菌并确定其分子特征

Barbara C. Weck , Adriana Santodomingo , Maria Carolina A. Serpa , Glauber M.B. de Oliveira , Felipe R. Jorge , Sebastián Muñoz-Leal , Marcelo B. Labruna
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引用次数: 0

摘要

本研究旨在通过分子方法检测、分离和鉴定巴西白耳负鼠(Didelphis albiventris)中的一种复发性热群(RFG)鲍氏菌。2015-2018年期间,在巴西圣保罗州的六个城市捕获了负鼠(Didelphis spp.),分子分析表明,在142只采样负鼠(感染率为4.9%)中,有7只负鼠(Didelphis albiventris)的血液中存在新型RFG鲍瑞氏杆菌。所有七只受感染的负鼠都来自一个地方(里贝朗普雷图市)。随后于2021年在里贝朗普雷图进行的实地研究中,又捕获了两只负鼠(D. albiventris),其中一只负鼠的血液中含有博氏病毒DNA。将这只受感染负鼠的浸渍组织接种到实验室动物(啮齿动物和兔子)和两只大耳负鼠(Didelphis aurita)身上,每天通过暗视野显微镜检查它们的血液样本。在实验动物的血液中未发现螺旋体。相反,两只负鼠在接种后 12 到 25 天的血液中发现了螺旋体。这些负鼠的血液样本被用于基于六个博氏体位点的多焦点测序分型(MLST)。根据 MLST 基因推断出的系统发育将测序后的包柔氏菌基因型定位为 RFG 包柔氏菌支系,与亚非组的包柔氏菌基本相同,并与另一个巴西分离株 "Candidatus B. caatinga "形成单系。根据这一系统发生分析,我们认为新分离的包柔氏菌属于一个假定的新物种,因此将其命名为 "Candidatus Borrelia mimona"。
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Isolation and molecular characterization of a novel relapsing fever group Borrelia from the white-eared opossum Didelphis albiventris in Brazil

This study aimed to detect, isolate and to characterize by molecular methods a relapsing fever group (RFG) Borrelia in white-eared opossums (Didelphis albiventris) from Brazil. During 2015–2018, when opossums (Didelphis spp.) were captured in six municipalities of the state of São Paulo, Brazil, molecular analyses revealed the presence of a novel RFG Borrelia sp. in the blood of seven opossums (Didelphis albiventris), out of 142 sampled opossums (4.9% infection rate). All seven infected opossums were from a single location (Ribeirão Preto municipality). In a subsequent field study in Ribeirão Preto during 2021, two new opossums (D. albiventris) were captured, of which one contained borrelial DNA in its blood. Macerated tissues from this infected opossum were inoculated into laboratory animals (rodents and rabbits) and two big-eared opossums (Didelphis aurita), which had blood samples examined daily via dark-field microscopy. No spirochetes were visualized in the blood of the laboratory animals. Contrastingly, spirochetes were visualized in the blood of the two D. aurita opossums between 12 and 25 days after inoculation. Blood samples from these opossums were used for a multi-locus sequencing typing (MLST) based on six borrelial loci. Phylogenies inferred from MLST genes positioned the sequenced Borrelia genotype into the RFG borreliae clade basally to borreliae of the Asian-African group, forming a monophyletic group with another Brazilian isolate, “Candidatus B. caatinga”. Based on this concatenated phylogenetic analysis, which supports that the new borrelial isolate corresponds to a putative new species, we propose the name “Candidatus Borrelia mimona”.

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