Stephanie H. Ayotte , Sarah J. Wallace , Christopher R. Allen , Kela P. Weber , Otto R. Stein , Ellen G. Lauchnor
{"title":"两级处理湿地中的微生物群落动态:处理季节性滑雪胜地废水的启示","authors":"Stephanie H. Ayotte , Sarah J. Wallace , Christopher R. Allen , Kela P. Weber , Otto R. Stein , Ellen G. Lauchnor","doi":"10.1016/j.biteb.2024.101885","DOIUrl":null,"url":null,"abstract":"<div><p>Microbial community studies in treatment wetlands (TW) have not investigated cold-climate TW and have focused primarily on laboratory mesocosms. This study determined microbial community composition and dynamics using 16s rRNA sequencing in a partially-saturated two-stage, vertical-flow pilot TW treating ski resort wastewater from December–April. Indicator species analysis found organisms capable of the primary nitrogen transformations observed in each stage at 3 °C, with nitrifiers in the unsaturated second stage and heterotrophic denitrifiers in the first stage where recycled nitrate is removed. During seasonal TW operation, microbial communities developed significant differences between TW stages and α-diversity decreased. Microbial communities were compared to batch mesocosm TW communities, which were significantly different from the pilot. The study is the first to discern community dynamics and key microorganisms during seasonal loading of a cold-climate, field-scale TW. Results raise questions regarding comparability of mesocosms to pilot systems and indicate more field-scale TW investigations are needed.</p></div>","PeriodicalId":8947,"journal":{"name":"Bioresource Technology Reports","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microbial community dynamics in a two-stage treatment wetland: Insights from treating seasonal ski resort wastewater\",\"authors\":\"Stephanie H. Ayotte , Sarah J. Wallace , Christopher R. Allen , Kela P. Weber , Otto R. Stein , Ellen G. Lauchnor\",\"doi\":\"10.1016/j.biteb.2024.101885\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Microbial community studies in treatment wetlands (TW) have not investigated cold-climate TW and have focused primarily on laboratory mesocosms. This study determined microbial community composition and dynamics using 16s rRNA sequencing in a partially-saturated two-stage, vertical-flow pilot TW treating ski resort wastewater from December–April. Indicator species analysis found organisms capable of the primary nitrogen transformations observed in each stage at 3 °C, with nitrifiers in the unsaturated second stage and heterotrophic denitrifiers in the first stage where recycled nitrate is removed. During seasonal TW operation, microbial communities developed significant differences between TW stages and α-diversity decreased. Microbial communities were compared to batch mesocosm TW communities, which were significantly different from the pilot. The study is the first to discern community dynamics and key microorganisms during seasonal loading of a cold-climate, field-scale TW. Results raise questions regarding comparability of mesocosms to pilot systems and indicate more field-scale TW investigations are needed.</p></div>\",\"PeriodicalId\":8947,\"journal\":{\"name\":\"Bioresource Technology Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-06-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioresource Technology Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589014X24001269\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Environmental Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589014X24001269","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Environmental Science","Score":null,"Total":0}
Microbial community dynamics in a two-stage treatment wetland: Insights from treating seasonal ski resort wastewater
Microbial community studies in treatment wetlands (TW) have not investigated cold-climate TW and have focused primarily on laboratory mesocosms. This study determined microbial community composition and dynamics using 16s rRNA sequencing in a partially-saturated two-stage, vertical-flow pilot TW treating ski resort wastewater from December–April. Indicator species analysis found organisms capable of the primary nitrogen transformations observed in each stage at 3 °C, with nitrifiers in the unsaturated second stage and heterotrophic denitrifiers in the first stage where recycled nitrate is removed. During seasonal TW operation, microbial communities developed significant differences between TW stages and α-diversity decreased. Microbial communities were compared to batch mesocosm TW communities, which were significantly different from the pilot. The study is the first to discern community dynamics and key microorganisms during seasonal loading of a cold-climate, field-scale TW. Results raise questions regarding comparability of mesocosms to pilot systems and indicate more field-scale TW investigations are needed.