吡咯糖基-tRNA 合成酶的进化：从甲烷生成到遗传密码扩展

IF 51.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Chemical Reviews Pub Date : 2024-07-02 DOI:10.1021/acs.chemrev.4c00031

Nikolaj G. Koch, Nediljko Budisa

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引用次数: 0

摘要

20 多年前，人们在特定的古细菌中发现了吡咯赖氨酸编码翻译系统。我们的综述概述了曾经默默无闻的吡咯赖氨酸-tRNA 合成酶（PylRS）tRNA 对如何为新兴的遗传密码扩展领域奠定了基础。我们的主要重点是讨论如何成功改造 PylRS，使其识别新底物并表现出更高的体内活性。我们汇编了一份可与 PylRS 系统结合的 ncAAs 综合清单。此外，我们还总结了 PylRS 系统最近在创造创新治疗方案方面的成功应用，如新型抗体-药物共轭物、疫苗模式的进步以及新型抗菌药的潜在生产。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Evolution of Pyrrolysyl-tRNA Synthetase: From Methanogenesis to Genetic Code Expansion

Over 20 years ago, the pyrrolysine encoding translation system was discovered in specific archaea. Our Review provides an overview of how the once obscure pyrrolysyl-tRNA synthetase (PylRS) tRNA pair, originally responsible for accurately translating enzymes crucial in methanogenic metabolic pathways, laid the foundation for the burgeoning field of genetic code expansion. Our primary focus is the discussion of how to successfully engineer the PylRS to recognize new substrates and exhibit higher in vivo activity. We have compiled a comprehensive list of ncAAs incorporable with the PylRS system. Additionally, we also summarize recent successful applications of the PylRS system in creating innovative therapeutic solutions, such as new antibody–drug conjugates, advancements in vaccine modalities, and the potential production of new antimicrobials.

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来源期刊

Chemical Reviews 化学-化学综合

CiteScore

106.00

自引率

1.10%

发文量

278

审稿时长

4.3 months

期刊介绍： Chemical Reviews is a highly regarded and highest-ranked journal covering the general topic of chemistry. Its mission is to provide comprehensive, authoritative, critical, and readable reviews of important recent research in organic, inorganic, physical, analytical, theoretical, and biological chemistry. Since 1985, Chemical Reviews has also published periodic thematic issues that focus on a single theme or direction of emerging research.

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