无牙周炎吸烟者的唾液氧化应激生物标志物:一项横断面研究。

IF 1.1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE Minerva dental and oral science Pub Date : 2024-08-01 Epub Date: 2024-07-02 DOI:10.23736/S2724-6329.24.04879-4
Mohammad S Alrashdan, Hisham Al-Shorman, Ahmed Al-Dwairi, Abubaker Qutieshat, Mahmoud K Al-Omiri
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引用次数: 0

摘要

背景:人们对唾液氧化应激进行了广泛的研究,试图将氧化应激标志物的变化与包括吸烟在内的局部和全身因素联系起来。本研究的目的是评估香烟和水烟(WPS)这两种吸烟形式对唾液中某些氧化应激生物标志物的影响:研究共招募了三组参与者,即对照组(从不吸烟者)、吸烟者和水烟吸烟者。参与者在临床上没有牙周炎和已知会影响唾液成分的全身性疾病。按照标准方案收集未刺激的全唾液样本,并检测 8-羟基-2'-脱氧鸟苷(8-OHdG)、髓过氧化物酶(MPO)、丙二醛(MDA)、总抗氧化能力(TAC)和皮质醇的浓度。采用单因素方差分析来比较三个研究组之间各氧化应激生物标志物的水平,并采用层次线性回归分析来检验唾液皮质醇水平对其他氧化应激生物标志物的预测作用。显著性水平设定为 95% 置信区间,概率值≤0.05:8-OHdG在WPS组最高(mean±SE 11,030.35±1829.16 pg/mL),而MDA和皮质醇水平在吸烟者组最高(mean±SE分别为3.33±0.52 µM和3.99±0.48 ng/mL),MPO在对照组最高(mean±SE 7.760±1.55 ng/mL)。WPS 组的 TAC 值最高(mean±SE 0.3±0.03 mM)。然而,所有测试的制造商均未达到具有统计学意义的差异:结论:尽管某些生物标志物发生了微妙的变化,但在无牙周炎的吸烟者中,唾液氧化应激似乎并未受到吸烟习惯的显著影响。
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Salivary oxidative stress biomarkers in periodontitis-free smokers: a cross sectional study.

Background: Salivary oxidative stress has been extensively studied with attempts to correlate changes in the oxidative stress markers with local and systemic factors, including smoking. The objective of this study was to evaluate the influence of two forms of smoking, cigarettes and waterpipe smoking (WPS), on selected oxidative stress biomarkers in saliva.

Methods: Three groups of participants were enrolled into the study, controls (never smokers), cigarette smokers and WPS. Participants were clinically free from periodontitis and systemic conditions known to affect the saliva constituents. Unstimulated whole saliva samples were collected according to a standard protocol and concentrations of 8-hydroxy-2'-deoxyguanosine (8-OHdG), myeloperoxidase (MPO), malondialdehyde (MDA), total antioxidant capacity (TAC), and cortisol. The one-way ANOVA test was used to compare the levels of each oxidative stress biomarker between the three study groups and the hierarchical linear regression analysis was used to test the levels of salivary cortisol for prediction of other oxidative stress biomarkers. Significance levels were set at 95% confidence intervals and probability values ≤0.05.

Results: 8-OHdG was highest in WPS group (mean±SE 11,030.35±1829.16 pg/mL) while MDA and cortisol levels were highest in the cigarette smokers group (mean±SE 3.33±0.52 µM and 3.99±0.48 ng/mL, respectively) and MPO was highest in the control group (mean±SE 7.760±1.55 ng/mL). WPS group showed the highest TAC (mean±SE 0.3±0.03 mM). However, none of the tested makers reached a statistically significant difference.

Conclusions: Despite subtle changes in some biomarkers, the salivary oxidative stress does not appear to be significantly influenced by smoking habits in periodontitis-free smokers.

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来源期刊
Minerva dental and oral science
Minerva dental and oral science DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.50
自引率
5.00%
发文量
61
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