Minerva dental and oral science最新文献

Background: This paper describes pharmacoeconomic analysis of ethanol and benzalkonium chloride disinfectants used in dental institutions to prevent infections. Pathogens can be transmitted to patients via air, items, contact or vectors. The aim of this study is to compare the efficiency and cost-effectiveness of both disinfectants.

Methods: For pharmacoeconomic assessment, cost minimization analysis, cost benefit analysis (CBA), cost effectiveness analysis and cost utility analysis were performed. The cost of disinfectants used in hand disinfection of dental professionals is estimated to be 50 times higher when using ethanol. Compared monthly costs for disinfectants in surface disinfection are 18 times higher when using ethanol.

Results: Results of CBA imply 12 hours as annual time needed for performance of benzalkonium chloride disinfection, and 720 hours for ethanol. Reduction of pathogens on the examined surface after application of benzalkonium chloride was 99-99% for all tested pathogens. The application of the amount of benzalkonium chloride analogous to the cost of ethanol in dental facilities could eliminate the chance of nosocomial infections.

Conclusions: The cost-effectiveness of benzalkonium chloride leads to more agile recovery of the patient. Performed assessments lead to the conclusion that benzalkonium chloride is more efficient in dental facilities than ethanol. Utilization of benzalkonium chloride improves quality of life, significantly decreasing time spent for application and frequent reapplications of the disinfectant.

Social relationships, self-esteem, and one's own view are all greatly impacted by smile aesthetics. Smile harmony can be upset by abnormalities like peg-shaped lateral incisors, which leads patients to look for the least invasive yet effective cosmetic procedures. Direct composite veneers are a conservative way to improve your appearance without requiring a lot of dental preparation. This research investigates clinical application, material improvements, and overall patient satisfaction of direct composite veneers in the context of esthetic smile rehabilitation. The outcomes of recent research on direct composite veneers, with an emphasis on minimally invasive cosmetic rehabilitation methods, are summarized in this narrative overview of the literature. Through extensive searches in PubMed, Scopus, and ScienceDirect, studies were chosen using keywords like "direct composite veneers," "esthetic restoration," and "minimally invasive dentistry." The inclusion of articles was determined by their applicability to patient satisfaction, clinical results, and composite material improvements. According to the review, direct composite veneers are a practical, affordable, and reversible substitute for porcelain veneers. Benefits include instant results, little tooth preparation, and the use of multilayer composite procedures to provide natural-looking results. Nevertheless, drawbacks like staining vulnerability, decreased durability, and upkeep needs highlight the necessity of cautious patient selection. Direct composite veneers are becoming more and more feasible for long-term aesthetic solutions because to recent developments in composite resins, such as nanohybrid and nanofilled technologies, which have improved their polishability, durability, and color stability. Direct composite veneers are a good choice for those looking for a customizable, minimally intrusive method of improving the appearance of their smiles. Composite veneers are a useful and versatile option for improving appearance, even though they could need upkeep over time. To increase their lifetime and expand their use in cosmetic dentistry, more study on material advancements is necessary.

Background: Although uncommon, adverse local and systemic reactions to local anesthetic administration can occurred. The postanesthetic necrosis is a rare complication which usually involved the palatal region. The wound healing is a complex process regulated by a range of growth factors. Concentrated growth factor (CGF) is the latest generation of autologous platelet concentrate which has been widely used in the repair and regeneration of oral soft and hard tissue. The study aims to analyses the clinical benefit of CGF in the treatment of postanesthetic necrosis of the hard palate.

Methods: The study included all patients referred to the Department of Maxillo-Facial Surgery, Marche University General Hospital, between 2020 and 2022, for aseptic ulcer of the hard palate due to local anesthetic administration. For each patient, the surgical debridement of the necrotic tissue and a peripheral blood sample were performed. The CGF was obtained by a centrifuge device and sutured in the affected site. The greasy gauze, placed to prevent the matrix detachment, was removed seven days post-operative.

Results: Seven patients (M:F=4:3, mean age 48.6±5.1 years) were enrolled in the study. After 7 days post-operative, no infection, discomfort, or dysphagia were reported. Moreover, 4 patients (57%) showed a total re-epithelialization, while 3 patients (43%) with larger defects, displayed a partial re-epithelialization. A complete recovery occurred in all cases 15 days after surgery.

Conclusions: CFG represents a promising potential preparation for the oral mucosa wound healing able to promote the granular tissue formation and the mucosa re-epithelialization.

Introduction: Obstructive sleep apnea syndrome (OSAS) is a breathing disorder during sleep, characterized by upper airway obstruction with many subsequent daytime and nocturnal symptoms. Children with OSAS may show abnormalities of the maxillary structure. This review aims to evaluate the effectiveness of rapid maxillary expansion (RME) as a treatment for OSAS in children to reduce apnea/hypopnea index and oxygen saturation.

Evidence acquisition: An electronic search for all articles published in English from January 2004 to February 2022. The study focused on children between 5 and 12 years old and meeting the following inclusion criteria: clinical signs of malocclusion, no syndromic children, adenotonsillar hypertrophy, underwent an otolaryngology evaluation and no previous treatment for OSAS. The device was fixed to the second deciduous molars. After 12 months, the RME was removed.

Evidence synthesis: The investigation recorded a statistically significant reduction in AHI (apnea/hypopnea index) (on average 60-65%) and an increase in oxygen saturation ranging from 95% to 97%.

Conclusions: This review shows that RME, through the enlargement of dental arches and nasal-maxillary structures, may be a helpful approach in children with malocclusion and OSAS.

Background: Understanding healing of the alveolar process is crucial for immediate implant, alveolar ridge preservation and guided bone regeneration procedures, and to evaluate it several different scales have been proposed; however, all have different characteristics and seem to miss a standardization allowing for an objective and dichotomous evaluation of the different aspects of wound healing. The objective of the present study is to propose and apply, in real clinical scenarios, a novel index for the objective evaluation of wound healing following erupted tooth extraction.

Methods: Healthy patients in need of a single tooth extraction were enrolled and re-examined at 7, 14 and 21 days after the extraction using the novel index proposed. For each follow up, a score ranging from 0 to 10 based on dichotomous factors (0-1) was obtained.

Results: Ten patients were enrolled in the study, 7 females and 3 males; the scores obtained at 21 days were coherent with the clinical appearance of the wound, and an excellent wound healing was obtained for everyone, except for two patients.

Conclusions: The proposed scale seems to accurately indicate the actual clinical status of the post-extraction wound and provide useful information on overall wound healing. Further validation of the scale is needed in future studies.

Alginates are widely used in dentistry for various applications due to their favorable properties, including ease of use, cost-effectiveness, and patient comfort. They are commonly employed for preliminary impressions of oral structures in dental practice. This study aims to assess the impact of different factors on the performance of alginate impression materials in dentistry. The mixing process of alginate involves combining the powder with water, and the technique employed for mixing can significantly affect the quality and porosity of the resulting material. The study employed a comprehensive search strategy using electronic databases and manual searches to gather relevant studies published in English. The results indicated that the amount of water used for mixing and the presence of calcium ions could affect the setting time, hardness, and elasticity of alginate impressions. Proper water-powder ratio and manipulation instructions are crucial in minimizing distortion in the resulting casts. Disinfectants like chlorhexidine can effectively reduce microorganism percentages on the impression surface without compromising quality. Various mixing techniques were evaluated, including manual spatulation, automated spinning bowl, centrifugal mixer, and vacuum mixer. Centrifugal and vacuum mixing showed better surface and internal porosity results-mechanical mixing techniques, such as vacuum mixers, improved performance.

Background: Cadaverine and hydrocinnamic acid are frequent metabolites in inflamed periodontal areas. Their role as a metabolite for plant growth inhibition has been established, but their relevance in humans has yet to be determined. Moreover, Vascular endothelial growth factor (VGEF) is a consistent growth factor in neo-angiogenesis in periodontal regeneration. The aim of the study was to utilize an in-silico approach to investigate the potential interaction between Cadaverine and hydrocinnamic acid, metabolites found in inflamed periodontal areas, and vascular endothelial growth factor (VEGF), with a focus on understanding their role in periodontal regeneration.

Methods: Desmond MD simulation is an efficient technique for analyzing the dynamics of protein-ligand complexes. The system is minimized and equilibrated after the protein-ligand combination has been solvated in a water box. The system is simulated for a desired time, typically 10-100 nanoseconds. The simulation data is examined to reveal the interactions between proteins and ligands, such as binding affinities, contact maps, and hydrogen bonding patterns. VEGF interactome of metabolites was assessed.

Results: Docking interactions between hydrocinnamic acid and VEGF with binding energy -5.0 kcal/mol and docking interactions between Cadaverine and VEGF with -3.6 kcal/mol. Fluctuations in RMSD values remain within 2.0 for the simulation duration, which is perfectly fine. Ligand RMSD values fluctuated within 1.0 Angstrom up to 25 ns, flipped in ligand mode, regained equilibrium at 80 ns, and remained steady for the simulation duration.

Conclusions: The current in-silico study suggests that metabolites like Cadaverine and hydrocinnamic acid, which are produced during periodontal inflammation, may have the ability to block pro-angiogenic vascular endothelial growth factors. This interference can have notable effects on the healing and regeneration of tissues by preventing the formation of blood vessels and the expression of VEGF.

Introduction: The aim of this study was to evaluate the long-term treatment outcomes of basal implants in patients with severely resorbed ridges, including the survival and success rates, patient complaints, satisfaction, and Quality of Life.

Evidence acquisition: An extensive electronic search was conducted on the search engines: PubMed, Web of Science, and Google Scholar using Boolean Operators (AND, OR, NOT) and the key words (basal implants, Corticobasal implants, Strategic Implants, severely resorbed ridge, severely atrophic ridge, treatment outcome, patient satisfaction) within the last 10 years.

Evidence synthesis: A total of 21 articles were found, encompassing 9732 basal implants placed in 1219 patients. Thirteen studies had reported a success or survival rate with a range of 90.3-100% for intraoral basal implants and 88.2% and 92.9% for orbital and nasal implants, respectively. Four studies have reported failure rates with a range of 0.3-3.2%, while seven articles documented 0.3-2.4% mobility. The pain was reported in 6 studies (0.3-0.8), marginal bone loss (0.33-7.89 mm), an increase in bone density, and peri-implant bone contact were reported in 3 and 2 studies, respectively.

Conclusions: Basal implant-supported prostheses can be a practical treatment modality with high predictable survival or success rates, positive impacts on patient satisfaction, and improved quality of life.

Background: Boswellic acid (BA) is a bioactive compound derived from Boswellia trees. This study aims to investigate the anti-cancer properties of BA against KB oral squamous cancer cells and elucidate the underlying mechanisms.

Methods: Escalating doses of BA were administered to KB cells, and various analyses were conducted using bioinformatic tools such as GEO, GEO2R, and STITCH database. MTT and trypan blue assays has been validated to measure the cytotoxicity by treating BA in KB Cells. Flow cytometry assessed cell cycle progression, apoptosis induction, and metabolic alterations. Network analysis identified relevant signaling pathways, while RT-PCR validated mRNA expression changes. Docking studies by Autodock evaluated beta-BA's binding affinity with mTOR-mediated pathways.

Results: BA effectively hindered KB cell progression, inducing G0/G1 phase cell cycle arrest and apoptosis. It also inhibited aerobic glycolysis, a hallmark of oral cancer cells. Network analysis revealed involvement in apoptosis and mTOR targets. RT-PCR confirmed downregulation of genes associated with aerobic glycolysis and apoptosis. Docking studies indicated strong binding between BA and mTOR pathways.

Conclusions: BA shows promise in inhibiting KB oral squamous cancer cell growth. These findings underscore its potential as a treatment for oral cancer. Further research and clinical studies are needed to unlock its full therapeutic potential.

Chronic periodontitis is a prevalent inflammatory condition that affects both the oral health and systemic well-being of individuals, particularly those with type 2 diabetes mellitus (T2DM). Accurate diagnosis and evaluation of periodontal status are crucial for effective management of the disease. This narrative review aimed to compare the diagnostic and evaluative capabilities of conventional periodontal probing and salivary biomarkers in chronic periodontitis among individuals with T2DM. The available literature highlights the clinical utility of conventional periodontal probing as the gold standard for periodontal diagnosis and evaluation. It enables the assessment of clinical parameters such as probing depth, clinical attachment level, and bleeding on probing, providing valuable information about the severity and extent of periodontal disease. However, this approach has limitations, including its invasive nature, potential for examiner bias, and limited ability to detect early stages of periodontitis. In recent years, salivary biomarkers have emerged as promising diagnostic tools for periodontal diseases. Various biomarkers, such as inflammatory cytokines, matrix metalloproteinases, and host-derived enzymes, have shown potential in differentiating periodontal health from disease and assessing disease activity. Salivary biomarker analysis offers advantages such as non-invasiveness, ease of collection, and the potential for chair-side testing. However, standardization of collection protocols, biomarker panels, and interpretation criteria remain a challenge. This narrative review provides an overview of the strengths and limitations of conventional periodontal probing and salivary biomarkers in the diagnosis and evaluation of chronic periodontitis in individuals with T2DM. Both approaches have their unique advantages and contribute valuable information in clinical practice. Combining conventional periodontal probing with salivary biomarker analysis holds promise for enhancing the accuracy and precision of periodontal disease diagnosis and monitoring in individuals with T2DM.