TBP 有助于有丝分裂后的 RNA 聚合酶 I 转录。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-07-03 DOI:10.1080/15476286.2024.2375097
James Z J Kwan, Thomas F Nguyen, Sheila S Teves
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引用次数: 0

摘要

TATA-box 结合蛋白(TBP)是真核生物三大 RNA 聚合酶(Pol I、II 和 III)启动复合体中唯一常见的转录因子。虽然 TBP 在不同物种的三种 RNA Pols 的转录过程中起着核心作用,但在整个进化过程中出现的 TBP 旁系物增加了转录起始过程的复杂性。此外,最近的研究质疑了 TBP 在哺乳动物细胞中的中心地位,尤其是在 Pol II 转录中,但 TBP 及其旁系物在 Pol I 转录中的作用仍有待重新评估。在本报告中,我们发现在小鼠胚胎干细胞中,TBP定位在Pol I启动子上,而TBP旁系亲属TRF2仅与rDNA的间隔启动子有微弱联系,这表明它可能无法取代TBP进行Pol I转录。重要的是,急性 TBP 消耗并不会完全破坏 Pol I 在核糖体 RNA 基因上的占据或活性,但 TBP 在有丝分裂中的结合会导致 Pol I 在细胞分裂后有效地重新激活。这些发现为TBP在小鼠胚胎干细胞的Pol I转录中提供了更细致的作用。
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TBP facilitates RNA Polymerase I transcription following mitosis.

The TATA-box binding protein (TBP) is the sole transcription factor common in the initiation complexes of the three major eukaryotic RNA Polymerases (Pol I, II and III). Although TBP is central to transcription by the three RNA Pols in various species, the emergence of TBP paralogs throughout evolution has expanded the complexity in transcription initiation. Furthermore, recent studies have emerged that questioned the centrality of TBP in mammalian cells, particularly in Pol II transcription, but the role of TBP and its paralogs in Pol I transcription remains to be re-evaluated. In this report, we show that in murine embryonic stem cells TBP localizes onto Pol I promoters, whereas the TBP paralog TRF2 only weakly associates to the Spacer Promoter of rDNA, suggesting that it may not be able to replace TBP for Pol I transcription. Importantly, acute TBP depletion does not fully disrupt Pol I occupancy or activity on ribosomal RNA genes, but TBP binding in mitosis leads to efficient Pol I reactivation following cell division. These findings provide a more nuanced role for TBP in Pol I transcription in murine embryonic stem cells.

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来源期刊
RNA Biology
RNA Biology 生物-生化与分子生物学
CiteScore
8.60
自引率
0.00%
发文量
82
审稿时长
1 months
期刊介绍: RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy
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