E. M. Moiseeva, V. V. Fadeev, Yu. V. Fadeeva, Yu. S. Gusev, M. I. Chumakov
{"title":"玉米原生质体中核糖核蛋白复合物对 GEX2 基因的 CRISPR/Cas9 编辑效果分析","authors":"E. M. Moiseeva, V. V. Fadeev, Yu. V. Fadeeva, Yu. S. Gusev, M. I. Chumakov","doi":"10.1134/s1022795424700285","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">\n<b>Abstract</b>—</h3><p>The GEX2 protein is expressed in the maize gamete membranes and is necessary during fertilization at the stage of contact (adhesion) of gamete membranes. Knockout of <i>GEX2</i> gene can presumably lead to impaired fertilization and, as a result, to the formation of matroclinic haploid maize embryos. The purpose of this study is to analyze the efficiency of CRISPR/Cas9 editing of the <i>GEX2</i> gene after PEG-mediated transfection of maize protoplasts by ribonucleoprotein (RNP) complexes with different gRNAs. For the first time, constructs for CRISPR/Cas9 editing of the maize <i>GEX2</i> gene have been created, the effectiveness of which has been proven on protoplasts and reaches 10.7%, depending on the selected gRNA and the ratio and number of components in the RNP complexes.</p>","PeriodicalId":21441,"journal":{"name":"Russian Journal of Genetics","volume":"11 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of the Effectiveness of CRISPR/Cas9-Editing of the GEX2 Gene by Ribonucleoprotein Complexes in Maize Protoplasts\",\"authors\":\"E. M. Moiseeva, V. V. Fadeev, Yu. V. Fadeeva, Yu. S. Gusev, M. I. Chumakov\",\"doi\":\"10.1134/s1022795424700285\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">\\n<b>Abstract</b>—</h3><p>The GEX2 protein is expressed in the maize gamete membranes and is necessary during fertilization at the stage of contact (adhesion) of gamete membranes. Knockout of <i>GEX2</i> gene can presumably lead to impaired fertilization and, as a result, to the formation of matroclinic haploid maize embryos. The purpose of this study is to analyze the efficiency of CRISPR/Cas9 editing of the <i>GEX2</i> gene after PEG-mediated transfection of maize protoplasts by ribonucleoprotein (RNP) complexes with different gRNAs. For the first time, constructs for CRISPR/Cas9 editing of the maize <i>GEX2</i> gene have been created, the effectiveness of which has been proven on protoplasts and reaches 10.7%, depending on the selected gRNA and the ratio and number of components in the RNP complexes.</p>\",\"PeriodicalId\":21441,\"journal\":{\"name\":\"Russian Journal of Genetics\",\"volume\":\"11 1\",\"pages\":\"\"},\"PeriodicalIF\":0.6000,\"publicationDate\":\"2024-06-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Russian Journal of Genetics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1134/s1022795424700285\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"GENETICS & HEREDITY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Russian Journal of Genetics","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s1022795424700285","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}
Analysis of the Effectiveness of CRISPR/Cas9-Editing of the GEX2 Gene by Ribonucleoprotein Complexes in Maize Protoplasts
Abstract—
The GEX2 protein is expressed in the maize gamete membranes and is necessary during fertilization at the stage of contact (adhesion) of gamete membranes. Knockout of GEX2 gene can presumably lead to impaired fertilization and, as a result, to the formation of matroclinic haploid maize embryos. The purpose of this study is to analyze the efficiency of CRISPR/Cas9 editing of the GEX2 gene after PEG-mediated transfection of maize protoplasts by ribonucleoprotein (RNP) complexes with different gRNAs. For the first time, constructs for CRISPR/Cas9 editing of the maize GEX2 gene have been created, the effectiveness of which has been proven on protoplasts and reaches 10.7%, depending on the selected gRNA and the ratio and number of components in the RNP complexes.
期刊介绍：
Russian Journal of Genetics is a journal intended to make significant contribution to the development of genetics. The journal publishes reviews and experimental papers in the areas of theoretical and applied genetics. It presents fundamental research on genetic processes at molecular, cell, organism, and population levels, including problems of the conservation and rational management of genetic resources and the functional genomics, evolutionary genomics and medical genetics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
