{"title":"利用内生真菌 Nigrospora gullinensis 的外多糖轻松合成和稳定硒纳米粒子及其生物活性研究","authors":"Anmol Singh, Priyankar Dey, Hisaaki Mihara, Nagaraja Tejo Prakash, Ranjana Prakash","doi":"10.1007/s13399-024-05870-1","DOIUrl":null,"url":null,"abstract":"<p>The primary objective of this work was to investigate the synthesis and the bioactivity of SeNPs encapsulated with exopolysaccharide (EPS). The EPS was mainly composed of 395 ± 13.20 mg/g carbohydrate and 121 ± 3.21 mg/g protein; the following moiety was used for surface stabilization of selenium nanoparticles (SeNPs). Bioactive SeNPs, i.e., EPS-SeNPs, with an average size of 43.7 ± 13.7 nm, were successfully synthesized. The antibacterial bioactivity of EPS-SeNPs was seen to be enhanced against Gram-positive bacteria, viz., <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, and <i>Enterococcus faecalis</i>, as well as Gram-negative bacteria, such as <i>Escherichia coli</i> and <i>Salmonella enterica</i>. In addition, the study also demonstrated antifungal activity against <i>Alternaria alternata</i>, <i>Fusarium laterium</i>, and <i>Aspergillus niger</i>. The cytotoxic activity of synthesized EPS-SeNPs exhibited a dose-dependent response against liver carcinoma cell lines, i.e., the HepG2. These findings support the hypothesis that these nanoparticles hold potential as an emerging material in the field of therapeutics.</p>","PeriodicalId":488,"journal":{"name":"Biomass Conversion and Biorefinery","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Facile synthesis of selenium nanoparticles and stabilization using exopolysaccharide from endophytic fungus, Nigrospora gullinensis, and their bioactivity study\",\"authors\":\"Anmol Singh, Priyankar Dey, Hisaaki Mihara, Nagaraja Tejo Prakash, Ranjana Prakash\",\"doi\":\"10.1007/s13399-024-05870-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The primary objective of this work was to investigate the synthesis and the bioactivity of SeNPs encapsulated with exopolysaccharide (EPS). The EPS was mainly composed of 395 ± 13.20 mg/g carbohydrate and 121 ± 3.21 mg/g protein; the following moiety was used for surface stabilization of selenium nanoparticles (SeNPs). Bioactive SeNPs, i.e., EPS-SeNPs, with an average size of 43.7 ± 13.7 nm, were successfully synthesized. The antibacterial bioactivity of EPS-SeNPs was seen to be enhanced against Gram-positive bacteria, viz., <i>Bacillus subtilis</i>, <i>Staphylococcus aureus</i>, and <i>Enterococcus faecalis</i>, as well as Gram-negative bacteria, such as <i>Escherichia coli</i> and <i>Salmonella enterica</i>. In addition, the study also demonstrated antifungal activity against <i>Alternaria alternata</i>, <i>Fusarium laterium</i>, and <i>Aspergillus niger</i>. The cytotoxic activity of synthesized EPS-SeNPs exhibited a dose-dependent response against liver carcinoma cell lines, i.e., the HepG2. These findings support the hypothesis that these nanoparticles hold potential as an emerging material in the field of therapeutics.</p>\",\"PeriodicalId\":488,\"journal\":{\"name\":\"Biomass Conversion and Biorefinery\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-06-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomass Conversion and Biorefinery\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1007/s13399-024-05870-1\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENERGY & FUELS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomass Conversion and Biorefinery","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1007/s13399-024-05870-1","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENERGY & FUELS","Score":null,"Total":0}
Facile synthesis of selenium nanoparticles and stabilization using exopolysaccharide from endophytic fungus, Nigrospora gullinensis, and their bioactivity study
The primary objective of this work was to investigate the synthesis and the bioactivity of SeNPs encapsulated with exopolysaccharide (EPS). The EPS was mainly composed of 395 ± 13.20 mg/g carbohydrate and 121 ± 3.21 mg/g protein; the following moiety was used for surface stabilization of selenium nanoparticles (SeNPs). Bioactive SeNPs, i.e., EPS-SeNPs, with an average size of 43.7 ± 13.7 nm, were successfully synthesized. The antibacterial bioactivity of EPS-SeNPs was seen to be enhanced against Gram-positive bacteria, viz., Bacillus subtilis, Staphylococcus aureus, and Enterococcus faecalis, as well as Gram-negative bacteria, such as Escherichia coli and Salmonella enterica. In addition, the study also demonstrated antifungal activity against Alternaria alternata, Fusarium laterium, and Aspergillus niger. The cytotoxic activity of synthesized EPS-SeNPs exhibited a dose-dependent response against liver carcinoma cell lines, i.e., the HepG2. These findings support the hypothesis that these nanoparticles hold potential as an emerging material in the field of therapeutics.
期刊介绍:
Biomass Conversion and Biorefinery presents articles and information on research, development and applications in thermo-chemical conversion; physico-chemical conversion and bio-chemical conversion, including all necessary steps for the provision and preparation of the biomass as well as all possible downstream processing steps for the environmentally sound and economically viable provision of energy and chemical products.