比较转录组为了解橄榄树对寒冷胁迫的分子调控提供了新的视角。

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-08-03 DOI:10.1093/treephys/tpae075
Wenjun Wu, Chengying Jiang, Qianqian Wei, Ling He, Gaoming Jin, Yuming Zheng, Jianli Qi, Rong Zhang, Yufang Yao, Dongshi Wan, Jiaojiao Lv
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引用次数: 0

摘要

橄榄(Olea europaea. L)是一种具有重要经济价值的产油作物,对低温非常敏感,这严重限制了其产量和地理分布。然而,橄榄耐寒性的内在机制仍然难以捉摸。本研究对两个橄榄栽培品种的幼苗进行了冷冻实验(4 °C),结果发现,与 O. europaea cv. Leccino 相比,O. europaea cv. Arbequina 表现出更强的耐寒性,光合作用活性更高。转录组分析表明,叶绿素合成的主要调节因子--早期光诱导蛋白 1(ELIP1)被显著诱导,以保护低温下的光合作用。此外,加权基因共表达网络分析(WGCNA)、酵母单杂交(Y1H)和荧光素酶(LUC)实验证明,转录因子 bHLH66 通过与启动子中的 G-box motif 结合,成为 ELIP1 转录的重要调控因子。综上所述,我们的研究揭示了由 bHLH66- ELIP1 组成的新型转录模块在橄榄树适应寒冷胁迫过程中的作用。
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Comparative transcriptome provides new insights into the molecular regulation of olive trees to chilling stress.

Olive (Olea europaea L.), an economically important oil-producing crop, is sensitive to low temperature, which severely limits its productivity and geographical distribution. However, the underlying mechanism of cold tolerance in olive remains elusive. In this study, a chilling experiment (4 °C) on the living saplings of two olive cultivars revealed that O. europaea cv. Arbequina showed stronger cold tolerance with greater photosynthetic activity compared with O. europaea cv. Leccino. Transcriptome analyses revealed that early light-inducible protein 1 (ELIP1), the main regulator for chlorophyll synthesis, is dramatically induced to protect the photosynthesis at low temperatures. Furthermore, weighted gene co-expression network analysis, yeast one-hybrid and luciferase assays demonstrated that transcription factor bHLH66 serves as an important regulator of ELIP1 transcription by binding to the G-box motif in the promoter. Taken together, our research revealed a novel transcriptional module consisting of bHLH66-ELIP1 in the adaptation of olive trees to cold stress.

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来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
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