Yeisson Gutiérrez, Karen A Alarcón, Cristian Ortiz, Jenny M Santos-Holguín, Jennifer L García-Riaño, Cindy Mejía, Carol V Amaya, Liz Uribe-Gutiérrez
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Moreover, the key carbon sources that allow optimal growth of the strain were identified through metabolic profiling, including sucrose, D-mannose, and γ-amino-butyric acid. These findings offer strategic insights for scalability and formulation methodologies. Additionally, enzymatic analyses unveiled robust protease activity within Bv065, crucial for catalysing insect cuticle degradation and facilitating host penetration, thus accentuating its entomopathogenic potential. Subsequent evaluations exposed Bv065's pathogenicity against D. borassi, causing significant mortality within nine days of exposure, albeit exhibiting limited effectiveness against Rhynchophorus palmarum. This study underscores the importance of understanding optimal growth conditions and metabolic preferences of B. bassiana strains for developing effective biopesticides. 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Overall, this study contributes to the growing body of knowledge on entomopathogenic fungi and their pivotal role in biological control, offering nuanced perspectives on eco-friendly alternatives to conventional insecticidal interventions.</p>","PeriodicalId":23703,"journal":{"name":"World journal of microbiology & biotechnology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11226477/pdf/","citationCount":"0","resultStr":"{\"title\":\"Isolation and characterization of a native strain of the entomopathogenic fungus Beauveria bassiana for the control of the palm weevil Dynamis borassi (Coleoptera: Curculionidae) in the neotropics.\",\"authors\":\"Yeisson Gutiérrez, Karen A Alarcón, Cristian Ortiz, Jenny M Santos-Holguín, Jennifer L García-Riaño, Cindy Mejía, Carol V Amaya, Liz Uribe-Gutiérrez\",\"doi\":\"10.1007/s11274-024-04044-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study aimed to isolate and characterize a native strain of Beauveria bassiana, coded as Bv065, showcasing its potential as a biological control agent targeting the palm weevil Dynamis borassi. 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引用次数: 0
摘要
本研究的目的是分离并鉴定一株本地的 Bauveria bassiana 菌株(代号为 Bv065),以展示其作为针对棕榈象鼻虫(Dynamis borassi)的生物防治剂的潜力。该真菌源于哥伦比亚西南部采集的自然感染 D. borassi 的标本,经过分子鉴定后被确定为 B. bassiana,与已知参考菌株的序列相似度很高。生理特性分析表明,Bv065 能在 25 至 30 °C 的温度范围和 6 至 9 的 pH 值范围内生长。此外,通过新陈代谢分析还确定了该菌株最佳生长的关键碳源,包括蔗糖、D-甘露糖和γ-氨基丁酸。这些发现为可扩展性和配方方法提供了战略性见解。此外,酶分析还揭示了 Bv065 内部强大的蛋白酶活性,这对催化昆虫角质层降解和促进宿主穿透至关重要,从而增强了其昆虫病原潜力。随后的评估揭示了 Bv065 对 D. borassi 的致病性,在接触后九天内就会导致大量死亡,但对 Rhynchophorus palmarum 的致病性有限。这项研究强调了了解 B. bassiana 菌株的最佳生长条件和代谢偏好对开发有效生物杀虫剂的重要性。研究结果表明,Bv065 是新热带地区病虫害综合防治战略的一个有前途的候选菌株,特别是在椰子和桃椰树种植中控制棕榈象甲虫虫害方面。未来的研究方向包括完善大规模生产方法、配制新型给药系统以及开展全面的田间药效试验,以充分挖掘 Bv065 在促进可持续虫害管理实践方面的潜力。总之,这项研究有助于增进人们对昆虫病原真菌及其在生物防治中的关键作用的了解,为传统杀虫干预措施的生态友好型替代品提供了细致入微的视角。
Isolation and characterization of a native strain of the entomopathogenic fungus Beauveria bassiana for the control of the palm weevil Dynamis borassi (Coleoptera: Curculionidae) in the neotropics.
This study aimed to isolate and characterize a native strain of Beauveria bassiana, coded as Bv065, showcasing its potential as a biological control agent targeting the palm weevil Dynamis borassi. Originating from a naturally infected D. borassi specimen collected in southwestern Colombia, the fungus underwent molecular identification and was identified as B. bassiana, exhibiting high sequence similarity with known reference strains. The physiological characterization revealed that Bv065 thrived within a temperature range of 25 to 30 °C and a pH range of 6 to 9. Moreover, the key carbon sources that allow optimal growth of the strain were identified through metabolic profiling, including sucrose, D-mannose, and γ-amino-butyric acid. These findings offer strategic insights for scalability and formulation methodologies. Additionally, enzymatic analyses unveiled robust protease activity within Bv065, crucial for catalysing insect cuticle degradation and facilitating host penetration, thus accentuating its entomopathogenic potential. Subsequent evaluations exposed Bv065's pathogenicity against D. borassi, causing significant mortality within nine days of exposure, albeit exhibiting limited effectiveness against Rhynchophorus palmarum. This study underscores the importance of understanding optimal growth conditions and metabolic preferences of B. bassiana strains for developing effective biopesticides. The findings suggest Bv065 as a promising candidate for integrated pest management strategies in neotropical regions, particularly for controlling palm weevil infestations in coconut and peach palm cultivation. Future research avenues include refining mass production methodologies, formulating novel delivery systems, and conducting comprehensive field efficacy trials to unlock the full potential of Bv065 in fostering sustainable pest management practices. Overall, this study contributes to the growing body of knowledge on entomopathogenic fungi and their pivotal role in biological control, offering nuanced perspectives on eco-friendly alternatives to conventional insecticidal interventions.
期刊介绍:
World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology.
Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions.
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· Simple isolation of microbes from local sources
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· Data reporting on host response to microbes
· Optimization of a procedure
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