将原代小鼠肺肿瘤细胞培养成器官组织的简便操作方案

IF 2 3区 医学 Q2 ANATOMY & MORPHOLOGY Annals of Anatomy-Anatomischer Anzeiger Pub Date : 2024-07-04 DOI:10.1016/j.aanat.2024.152298
Jannis Ludwig , Felix Ritzmann , Andreas Kamyschnikow , Christian Herr , Robert Bals , Christoph Beisswenger
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引用次数: 0

摘要

癌症研究涉及大量的动物消耗和痛苦。肿瘤细胞可以在体外分化成与原发肿瘤相似的三维有机体。然而,在基础癌症研究中,肿瘤器官组织通常只用于动物实验。我们建立了一种易于操作的方案,可以将 KRAS 驱动的肺肿瘤细胞长期培养成器质体。与小鼠的相应肿瘤一样,器官组织也能产生表面活性蛋白 C,但没有气道上皮细胞的标记物(如 SCGB1A1、KRT5)。有机体可作为单细胞悬浮液进行传代。我们的类器官模型有助于用细胞培养系统取代动物实验,可用于癌症研究中的药物测试或功能研究。
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An easy-to-perform protocol for culturing primary murine lung tumor cells as organoids

Cancer research involves significant animal consumption and suffering. Tumor cells can be differentiated in vitro into three-dimensional organoids that resemble the primary tumor. In basic cancer research, however, tumor organoids are usually only used alongside animal experiments. We have established an easy-to-perform protocol that allows to culture KRAS-driven lung tumor cells as organoids for extended periods of time. Like the corresponding tumors in mice, the organoids produce surfactant protein C but no markers of airway epithelial cells (e.g. SCGB1A1, KRT5). The organoids can be passaged as single cell suspensions. Our organoid model contributes to replace animal experiments with cell culture systems and can be used for drug testing or functional studies in cancer research.

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来源期刊
Annals of Anatomy-Anatomischer Anzeiger
Annals of Anatomy-Anatomischer Anzeiger 医学-解剖学与形态学
CiteScore
4.40
自引率
22.70%
发文量
137
审稿时长
33 days
期刊介绍: Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as •molecular biology, •cell biology •reproductive biology •immunobiology •developmental biology, neurobiology •embryology as well as •neuroanatomy •neuroimmunology •clinical anatomy •comparative anatomy •modern imaging techniques •evolution, and especially also •aging
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