Jannis Ludwig , Felix Ritzmann , Andreas Kamyschnikow , Christian Herr , Robert Bals , Christoph Beisswenger
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An easy-to-perform protocol for culturing primary murine lung tumor cells as organoids
Cancer research involves significant animal consumption and suffering. Tumor cells can be differentiated in vitro into three-dimensional organoids that resemble the primary tumor. In basic cancer research, however, tumor organoids are usually only used alongside animal experiments. We have established an easy-to-perform protocol that allows to culture KRAS-driven lung tumor cells as organoids for extended periods of time. Like the corresponding tumors in mice, the organoids produce surfactant protein C but no markers of airway epithelial cells (e.g. SCGB1A1, KRT5). The organoids can be passaged as single cell suspensions. Our organoid model contributes to replace animal experiments with cell culture systems and can be used for drug testing or functional studies in cancer research.
期刊介绍:
Annals of Anatomy publish peer reviewed original articles as well as brief review articles. The journal is open to original papers covering a link between anatomy and areas such as
•molecular biology,
•cell biology
•reproductive biology
•immunobiology
•developmental biology, neurobiology
•embryology as well as
•neuroanatomy
•neuroimmunology
•clinical anatomy
•comparative anatomy
•modern imaging techniques
•evolution, and especially also
•aging