评估布里蒂(Mauritia flexuosa Liliopsida)和木薯(Manihot esculenta Crantz)残渣的化学成分及其在生物生产椰子香气(6-戊基-α-吡喃酮)中的可能应用。

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-10-01 Epub Date: 2024-07-06 DOI:10.1007/s00449-024-03055-8
A S Nascimento, U M Nascimento, G J Muchave, G E C Marques, G S Nascimento, C Mendonça, G S B Becco, C P Borges, S G F Leite
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引用次数: 0

摘要

这项工作旨在确定提高 6-戊基-α-吡喃酮(bioaroma)生物产量的战略。作为第一项战略,利用农用工业残留物在固态下进行发酵:Mauritia flexuosa Liliopsida.和 Manihot esculenta Crantz 分离出来,用不同的营养液进行发酵,发酵真菌为 Trichoderma harzianum。需要进行理化特性、百分数组成、木质纤维素和矿物质含量以及抗菌活性的分析。在不同的加湿条件下(水、不含添加剂的营养液和含葡萄糖或蔗糖的营养液)进行了为期 9 天的发酵。在固相微萃取法的辅助下,采用气相色谱法对生物醛进行定量。结果表明,在使用甜木薯进行的发酵中,这种化合物的产量很低(约为 6 ppm(w/w))。甜木薯残渣的生物产量低可能与其富含淀粉、基质均匀和营养浓度低有关。在使用布里提时,已经检测到没有香气产生。可能是布里提中硅的存在和木质素的高含量将真菌的活性降到了最低,从而难以获得所需的香气。鉴于废料的特性,我们选择了一种新的策略:以 1:1 的比例混合废料。使用添加了葡萄糖的营养液进行发酵,结果产生了 156.24 ppm(w/w)的香气。因此,可能是由于甜木薯中的可发酵糖和布里蒂果皮中的脂肪酸的存在,这种组合为工艺提供了更有利的环境,从而证明了生物生产椰子香气的可能性增加了约 2500%。
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Assessment of the chemical composition of buriti (Mauritia flexuosa Liliopsida) and cassava (Manihot esculenta Crantz) residues and their possible application in the bioproduction of coconut aroma (6 pentyl-α-pyrone).

This work aimed to define strategies to increase the bioproduction of 6 pentyl-α-pyrone (bioaroma). As first strategy, fermentations were carried out in the solid state, with agro-industrial residues: Mauritia flexuosa Liliopsida. and Manihot esculenta Crantz in isolation, conducting them with different nutrient solutions having Trichoderma harzianum as a fermenting fungus. Physicochemical characterizations, centesimal composition, lignocellulosic and mineral content and antimicrobial activity were required. Fermentations were conducted under different humidification conditions (water, nutrient solution without additives and nutrient solutions with glucose or sucrose) for 9 days. Bioaroma was quantified by gas chromatography, assisted by solid-phase microextraction. The results showed the low production of this compound in fermentations conducted with sweet cassava (around 6 ppm (w/w)). The low bioproduction with sweet cassava residues can probably be related to its starch-rich composition, homogeneous substrate, and low concentration of nutrients. Already using buriti, the absence of aroma production was detected. Probably the presence of silicon and high lignin content in buriti minimized the fungal activity, making it difficult to obtain the aroma of interest. Given the characteristics presented by the waste, a new strategy was chosen: mixing waste in a 1:1 ratio. This fermentation resulted in the production of 156.24 ppm (w/w) of aroma using the nutrient solution added with glucose. This combination, therefore, promoted more favorable environment for the process, possibly due to the presence of fermentable sugars from sweet cassava and fatty acids from the buriti peel, thus proving the possibility of an increase of around 2500% in the bioproduction of coconut aroma.

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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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