Liu Chang, Wang Shengdong, Zhang Huien, Liu Liping
{"title":"含有钙交联的醋酸壳聚糖海绵的抗炎和促进愈合作用","authors":"Liu Chang, Wang Shengdong, Zhang Huien, Liu Liping","doi":"10.1002/pat.6504","DOIUrl":null,"url":null,"abstract":"Chitosan, a potential material for tissue wound repair, exhibits limitations like poor solubility and low gel strength. Acetate chitosan (CA) was synthesized through ethanol precipitation, while acetate chitosan sponge (CA‐Ca) was created using the freeze‐drying method with CaCO<jats:sub>3</jats:sub> as cross‐linking agents. The addition of CaCO<jats:sub>3</jats:sub> enhanced the mechanical strength of the sponges formed by CA, but affected the water absorption performance of the sponge. Both CA and CA‐Ca demonstrated antioxidant properties, with CA‐Ca showing slightly higher maximal scavenging rates for DPPH and hydroxyl radicals compared to CA. Concentrations ranging from 1 to 500 μg/mL of CA and CA‐Ca exhibited a proliferative effect on L929 and RAW264.7 cells. Furthermore, both CA and CA‐Ca promoted the migration of L929 cells, with CA‐Ca showing a higher maximal healing rate within 24 h compared to the control group. Additionally, both materials reduced the levels of TNF‐αand IL‐6 in inflammatory RAW264.7 cells, thereby alleviating the inflammatory response. Moreover, CA and CA‐Ca stimulated collagen secretion in fibroblasts without inducing excessive secretion. CA sponge demonstrated the ability to accelerate in vitro coagulation, while CA‐Ca‐8 (CA:CaCO<jats:sub>3</jats:sub> 1:8) sponge, prepared with 2% CA, exhibited the most effective coagulation. Overall, CA‐Ca has suitable characteristics such as water absorption, coagulation, cytocompatibility, anti‐inflammatory properties, and promoting cell healing, laying the foundation for its potential clinical applications.","PeriodicalId":20382,"journal":{"name":"Polymers for Advanced Technologies","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Anti‐inflammatory and pro‐healing effects of acetate chitosan sponge with calcium cross‐links\",\"authors\":\"Liu Chang, Wang Shengdong, Zhang Huien, Liu Liping\",\"doi\":\"10.1002/pat.6504\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Chitosan, a potential material for tissue wound repair, exhibits limitations like poor solubility and low gel strength. Acetate chitosan (CA) was synthesized through ethanol precipitation, while acetate chitosan sponge (CA‐Ca) was created using the freeze‐drying method with CaCO<jats:sub>3</jats:sub> as cross‐linking agents. The addition of CaCO<jats:sub>3</jats:sub> enhanced the mechanical strength of the sponges formed by CA, but affected the water absorption performance of the sponge. Both CA and CA‐Ca demonstrated antioxidant properties, with CA‐Ca showing slightly higher maximal scavenging rates for DPPH and hydroxyl radicals compared to CA. Concentrations ranging from 1 to 500 μg/mL of CA and CA‐Ca exhibited a proliferative effect on L929 and RAW264.7 cells. Furthermore, both CA and CA‐Ca promoted the migration of L929 cells, with CA‐Ca showing a higher maximal healing rate within 24 h compared to the control group. Additionally, both materials reduced the levels of TNF‐αand IL‐6 in inflammatory RAW264.7 cells, thereby alleviating the inflammatory response. Moreover, CA and CA‐Ca stimulated collagen secretion in fibroblasts without inducing excessive secretion. CA sponge demonstrated the ability to accelerate in vitro coagulation, while CA‐Ca‐8 (CA:CaCO<jats:sub>3</jats:sub> 1:8) sponge, prepared with 2% CA, exhibited the most effective coagulation. Overall, CA‐Ca has suitable characteristics such as water absorption, coagulation, cytocompatibility, anti‐inflammatory properties, and promoting cell healing, laying the foundation for its potential clinical applications.\",\"PeriodicalId\":20382,\"journal\":{\"name\":\"Polymers for Advanced Technologies\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.1000,\"publicationDate\":\"2024-07-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Polymers for Advanced Technologies\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://doi.org/10.1002/pat.6504\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"POLYMER SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Polymers for Advanced Technologies","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1002/pat.6504","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"POLYMER SCIENCE","Score":null,"Total":0}
Anti‐inflammatory and pro‐healing effects of acetate chitosan sponge with calcium cross‐links
Chitosan, a potential material for tissue wound repair, exhibits limitations like poor solubility and low gel strength. Acetate chitosan (CA) was synthesized through ethanol precipitation, while acetate chitosan sponge (CA‐Ca) was created using the freeze‐drying method with CaCO3 as cross‐linking agents. The addition of CaCO3 enhanced the mechanical strength of the sponges formed by CA, but affected the water absorption performance of the sponge. Both CA and CA‐Ca demonstrated antioxidant properties, with CA‐Ca showing slightly higher maximal scavenging rates for DPPH and hydroxyl radicals compared to CA. Concentrations ranging from 1 to 500 μg/mL of CA and CA‐Ca exhibited a proliferative effect on L929 and RAW264.7 cells. Furthermore, both CA and CA‐Ca promoted the migration of L929 cells, with CA‐Ca showing a higher maximal healing rate within 24 h compared to the control group. Additionally, both materials reduced the levels of TNF‐αand IL‐6 in inflammatory RAW264.7 cells, thereby alleviating the inflammatory response. Moreover, CA and CA‐Ca stimulated collagen secretion in fibroblasts without inducing excessive secretion. CA sponge demonstrated the ability to accelerate in vitro coagulation, while CA‐Ca‐8 (CA:CaCO3 1:8) sponge, prepared with 2% CA, exhibited the most effective coagulation. Overall, CA‐Ca has suitable characteristics such as water absorption, coagulation, cytocompatibility, anti‐inflammatory properties, and promoting cell healing, laying the foundation for its potential clinical applications.
期刊介绍：
Polymers for Advanced Technologies is published in response to recent significant changes in the patterns of materials research and development. Worldwide attention has been focused on the critical importance of materials in the creation of new devices and systems. It is now recognized that materials are often the limiting factor in bringing a new technical concept to fruition and that polymers are often the materials of choice in these demanding applications. A significant portion of the polymer research ongoing in the world is directly or indirectly related to the solution of complex, interdisciplinary problems whose successful resolution is necessary for achievement of broad system objectives.
Polymers for Advanced Technologies is focused to the interest of scientists and engineers from academia and industry who are participating in these new areas of polymer research and development. It is the intent of this journal to impact the polymer related advanced technologies to meet the challenge of the twenty-first century.
Polymers for Advanced Technologies aims at encouraging innovation, invention, imagination and creativity by providing a broad interdisciplinary platform for the presentation of new research and development concepts, theories and results which reflect the changing image and pace of modern polymer science and technology.
Polymers for Advanced Technologies aims at becoming the central organ of the new multi-disciplinary polymer oriented materials science of the highest scientific standards. It will publish original research papers on finished studies; communications limited to five typewritten pages plus three illustrations, containing experimental details; review articles of up to 40 pages; letters to the editor and book reviews. Review articles will normally be published by invitation. The Editor-in-Chief welcomes suggestions for reviews.
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