转录组和代谢组的综合分析揭示了兼性盐生植物 Avicennia marina 根系对高低盐度的不同耐受机制。

IF 3.5 2区 农林科学 Q1 FORESTRY Tree physiology Pub Date : 2024-08-03 DOI:10.1093/treephys/tpae082
Jing Li, Chao-Qun Xu, Ling-Yu Song, Ze-Jun Guo, Lu-Dan Zhang, Han-Chen Tang, Ji-Cheng Wang, Shi-Wei Song, Jing-Wen Liu, You-Hui Zhong, Bing-Jie Chi, Xue-Yi Zhu, Hai-Lei Zheng
{"title":"转录组和代谢组的综合分析揭示了兼性盐生植物 Avicennia marina 根系对高低盐度的不同耐受机制。","authors":"Jing Li, Chao-Qun Xu, Ling-Yu Song, Ze-Jun Guo, Lu-Dan Zhang, Han-Chen Tang, Ji-Cheng Wang, Shi-Wei Song, Jing-Wen Liu, You-Hui Zhong, Bing-Jie Chi, Xue-Yi Zhu, Hai-Lei Zheng","doi":"10.1093/treephys/tpae082","DOIUrl":null,"url":null,"abstract":"<p><p>Mangroves perform a crucial ecological role along the tropical and subtropical coastal intertidal zone where salinity fluctuation occurs frequently. However, the differential responses of mangrove plant at the combined transcriptome and metabolome level to variable salinity are not well documented. In this study, we used Avicennia marina (Forssk.) Vierh., a pioneer species of mangrove wetlands and one of the most salt-tolerant mangroves, to investigate the differential salt tolerance mechanisms under low and high salinity using inductively coupled plasma-mass spectrometry, transcriptomic and metabolomic analysis. The results showed that HAK8 was up-regulated and transported K+ into the roots under low salinity. However, under high salinity, AKT1 and NHX2 were strongly induced, which indicated the transport of K+ and Na+ compartmentalization to maintain ion homeostasis. In addition, A. marina tolerates low salinity by up-regulating ABA signaling pathway and accumulating more mannitol, unsaturated fatty acids, amino acids' and L-ascorbic acid in the roots. Under high salinity, A. marina undergoes a more drastic metabolic network rearrangement in the roots, such as more L-ascorbic acid and oxiglutatione were up-regulated, while carbohydrates, lipids and amino acids were down-regulated in the roots, and, finally, glycolysis and TCA cycle were promoted to provide more energy to improve salt tolerance. Our findings suggest that the major salt tolerance traits in A. marina can be attributed to complex regulatory and signaling mechanisms, and show significant differences between low and high salinity.</p>","PeriodicalId":23286,"journal":{"name":"Tree physiology","volume":" ","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrative analysis of transcriptome and metabolome reveal the differential tolerance mechanisms to low and high salinity in the roots of facultative halophyte Avicennia marina.\",\"authors\":\"Jing Li, Chao-Qun Xu, Ling-Yu Song, Ze-Jun Guo, Lu-Dan Zhang, Han-Chen Tang, Ji-Cheng Wang, Shi-Wei Song, Jing-Wen Liu, You-Hui Zhong, Bing-Jie Chi, Xue-Yi Zhu, Hai-Lei Zheng\",\"doi\":\"10.1093/treephys/tpae082\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Mangroves perform a crucial ecological role along the tropical and subtropical coastal intertidal zone where salinity fluctuation occurs frequently. However, the differential responses of mangrove plant at the combined transcriptome and metabolome level to variable salinity are not well documented. In this study, we used Avicennia marina (Forssk.) Vierh., a pioneer species of mangrove wetlands and one of the most salt-tolerant mangroves, to investigate the differential salt tolerance mechanisms under low and high salinity using inductively coupled plasma-mass spectrometry, transcriptomic and metabolomic analysis. The results showed that HAK8 was up-regulated and transported K+ into the roots under low salinity. However, under high salinity, AKT1 and NHX2 were strongly induced, which indicated the transport of K+ and Na+ compartmentalization to maintain ion homeostasis. In addition, A. marina tolerates low salinity by up-regulating ABA signaling pathway and accumulating more mannitol, unsaturated fatty acids, amino acids' and L-ascorbic acid in the roots. Under high salinity, A. marina undergoes a more drastic metabolic network rearrangement in the roots, such as more L-ascorbic acid and oxiglutatione were up-regulated, while carbohydrates, lipids and amino acids were down-regulated in the roots, and, finally, glycolysis and TCA cycle were promoted to provide more energy to improve salt tolerance. Our findings suggest that the major salt tolerance traits in A. marina can be attributed to complex regulatory and signaling mechanisms, and show significant differences between low and high salinity.</p>\",\"PeriodicalId\":23286,\"journal\":{\"name\":\"Tree physiology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-08-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Tree physiology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/treephys/tpae082\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"FORESTRY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tree physiology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/treephys/tpae082","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FORESTRY","Score":null,"Total":0}
引用次数: 0

摘要

红树林在盐度波动频繁的热带和亚热带沿海潮间带发挥着重要的生态作用。然而,红树植物在转录组和代谢组水平上对盐度变化的不同反应还没有很好的记录。在本研究中,我们以红树林湿地的先锋物种、最耐盐的红树林之一 Avicennia marina 为研究对象,利用 ICP-MS、转录组和代谢组分析方法研究了其在低盐度和高盐度下的不同耐盐机制。结果表明,在低盐度条件下,HAK8上调并将K+运入根部。然而,在高盐度条件下,AKT1和NHX2被强烈诱导,这表明K+和Na+的运输分区可维持离子平衡。此外,马利筋草通过上调 ABA 信号通路和在根部积累更多的甘露醇、不饱和脂肪酸、氨基酸和 L-抗坏血酸来耐受低盐度。在高盐度条件下,A. marina 的根系发生了更剧烈的代谢网络重排,如更多的 L-抗坏血酸和氧化还原酶被上调,而碳水化合物、脂类和氨基酸在根系中被下调,最后促进糖酵解和 TCA 循环,以提供更多的能量来提高耐盐性。我们的研究结果表明,滨藜的主要耐盐性状可归因于复杂的调控和信号转导机制,并在低盐度和高盐度之间表现出显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Integrative analysis of transcriptome and metabolome reveal the differential tolerance mechanisms to low and high salinity in the roots of facultative halophyte Avicennia marina.

Mangroves perform a crucial ecological role along the tropical and subtropical coastal intertidal zone where salinity fluctuation occurs frequently. However, the differential responses of mangrove plant at the combined transcriptome and metabolome level to variable salinity are not well documented. In this study, we used Avicennia marina (Forssk.) Vierh., a pioneer species of mangrove wetlands and one of the most salt-tolerant mangroves, to investigate the differential salt tolerance mechanisms under low and high salinity using inductively coupled plasma-mass spectrometry, transcriptomic and metabolomic analysis. The results showed that HAK8 was up-regulated and transported K+ into the roots under low salinity. However, under high salinity, AKT1 and NHX2 were strongly induced, which indicated the transport of K+ and Na+ compartmentalization to maintain ion homeostasis. In addition, A. marina tolerates low salinity by up-regulating ABA signaling pathway and accumulating more mannitol, unsaturated fatty acids, amino acids' and L-ascorbic acid in the roots. Under high salinity, A. marina undergoes a more drastic metabolic network rearrangement in the roots, such as more L-ascorbic acid and oxiglutatione were up-regulated, while carbohydrates, lipids and amino acids were down-regulated in the roots, and, finally, glycolysis and TCA cycle were promoted to provide more energy to improve salt tolerance. Our findings suggest that the major salt tolerance traits in A. marina can be attributed to complex regulatory and signaling mechanisms, and show significant differences between low and high salinity.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Tree physiology
Tree physiology 农林科学-林学
CiteScore
7.10
自引率
7.50%
发文量
133
审稿时长
1 months
期刊介绍: Tree Physiology promotes research in a framework of hierarchically organized systems, measuring insight by the ability to link adjacent layers: thus, investigated tree physiology phenomenon should seek mechanistic explanation in finer-scale phenomena as well as seek significance in larger scale phenomena (Passioura 1979). A phenomenon not linked downscale is merely descriptive; an observation not linked upscale, might be trivial. Physiologists often refer qualitatively to processes at finer or coarser scale than the scale of their observation, and studies formally directed at three, or even two adjacent scales are rare. To emphasize the importance of relating mechanisms to coarser scale function, Tree Physiology will highlight papers doing so particularly well as feature papers.
期刊最新文献
Mechanistic Insights into Leaf Senescence Regulation in Woody Plants: A Molecular Perspective. CsCBF2 contributes to cold repression of chlorophyll and carotenoid biosynthesis in albino Camellia sinensis cv. Baiye 1. Uncovering protein regulation during adventitious root formation in Platycladus orientalis cuttings. Changes in morphological and physiological traits of urban trees in response to elevated temperatures within an Urban Heat Islands. Hydraulic and structural traits of trees across light gradients in the Amazon secondary forest.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1