酿酒酵母中剪接体介导的前 mRNA 剪接的机制和调控。

IF 6.4 2区 生物学 Q1 CELL BIOLOGY Wiley Interdisciplinary Reviews: RNA Pub Date : 2024-07-01 DOI:10.1002/wrna.1866
Katherine Anne Senn, Aaron A Hoskins
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引用次数: 0

摘要

前核糖核酸剪接,即去除内含子和连接侧翼外显子,是真核生物基因表达的关键步骤。剪接体是由五种小核 RNA(snRNA)和数十种蛋白质组成的大分子复合体,它通过复杂的途径在内含子上组装,然后催化剪接所需的两个酯化反应。所有这些步骤都有可能受到高度调控,以确保产生正确的 mRNA 异构体,从而实现适当的细胞功能。虽然酿酒酵母(酵母)的含内含子基因数量有限,但其中许多基因表达量很高,导致细胞中大量转录本被剪接。因此,剪接调控对酵母至关重要。与人类一样,酵母的剪接也会受到剪接机制的蛋白质成分、前核糖核酸本身的结构和特性或反式作用因子的影响。对酵母剪接调控机制和途径的进一步分析很可能揭示出适用于其他真核生物的一般原则。本文归类于RNA 处理 > 剪接机制 RNA 处理 > 剪接调节/替代剪接。
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Mechanisms and regulation of spliceosome-mediated pre-mRNA splicing in Saccharomyces cerevisiae.

Pre-mRNA splicing, the removal of introns and ligation of flanking exons, is a crucial step in eukaryotic gene expression. The spliceosome, a macromolecular complex made up of five small nuclear RNAs (snRNAs) and dozens of proteins, assembles on introns via a complex pathway before catalyzing the two transesterification reactions necessary for splicing. All of these steps have the potential to be highly regulated to ensure correct mRNA isoform production for proper cellular function. While Saccharomyces cerevisiae (yeast) has a limited set of intron-containing genes, many of these genes are highly expressed, resulting in a large number of transcripts in a cell being spliced. As a result, splicing regulation is of critical importance for yeast. Just as in humans, yeast splicing can be influenced by protein components of the splicing machinery, structures and properties of the pre-mRNA itself, or by the action of trans-acting factors. It is likely that further analysis of the mechanisms and pathways of splicing regulation in yeast can reveal general principles applicable to other eukaryotes. This article is categorized under: RNA Processing > Splicing Mechanisms RNA Processing > Splicing Regulation/Alternative Splicing.

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来源期刊
CiteScore
14.80
自引率
4.10%
发文量
67
审稿时长
6-12 weeks
期刊介绍: WIREs RNA aims to provide comprehensive, up-to-date, and coherent coverage of this interesting and growing field, providing a framework for both RNA experts and interdisciplinary researchers to not only gain perspective in areas of RNA biology, but to generate new insights and applications as well. Major topics to be covered are: RNA Structure and Dynamics; RNA Evolution and Genomics; RNA-Based Catalysis; RNA Interactions with Proteins and Other Molecules; Translation; RNA Processing; RNA Export/Localization; RNA Turnover and Surveillance; Regulatory RNAs/RNAi/Riboswitches; RNA in Disease and Development; and RNA Methods.
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