BYSL在黑色素瘤中的表达增加及其预后意义

IF 3.2 4区 医学 Q3 IMMUNOLOGY Journal of Immunotherapy Pub Date : 2024-10-01 Epub Date: 2024-07-09 DOI:10.1097/CJI.0000000000000530
Zhong-Zhi Wang, Guo-Tai Yao, Liang-Zhe Wang, Yuan-Jie Zhu, Jiang-Han Chen
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引用次数: 0

摘要

我们评估了黑色素瘤(SKCM)总生存率(OS)中 BYSL 的含量及其内在机制。在这项研究中,我们采用了一种结合生物信息学工具的综合方法,包括 miRNA 估算、miRNA 的定量实时聚合酶链反应(qRT-PCR)、E3 连接酶估算、STRING 分析、TIMER 分析、相关上游调节因子检查、蛋白-蛋白相互作用(PPI)分析、回顾性分析和生存分析,以及临床样本验证。这些方法用于研究 BYSL 的含量、甲基化状态、与患者预后的关系以及在肿瘤中的免疫学意义。我们的研究结果表明,BYSL的表达受BYSL甲基化的负调控。对468例SKCM RNA测序样本的分析表明,BYSL表达的增强与肿瘤分级较高有关。我们发现了几个与BYSL水平密切相关的miRNA,即hsa-miR-146b-3p、hsa-miR-342-3p、hsa-miR-511-5p、hsa-miR-3690和hsa-miR-193a-5p。此外,我们还预测了BYSL的E3泛素连接酶,并发现CBL、FBXW7、FZR1、KLHL3和MARCH1是BYSL的潜在调节因子。通过调查,我们发现 PNO1、RIOK2、TSR1、WDR3 和 NOB1 蛋白与 BYSL 的表达密切相关。此外,我们还发现 BYSL 水平与某些免疫细胞,尤其是树突状细胞(DCs)密切相关。值得注意的是,我们观察到在 SKCM 血清样本中,miR-146b-3p 与 BYSL mRNA 表达呈显著负相关。总之,根据之前显示的证据,BYSL 可作为 SKCM 患者预后的可靠生物指标,而且它有可能导致 SKCM 中免疫细胞的入侵。
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Increased Expression and Prognostic Significance of BYSL in Melanoma.

We evaluated the BYSL content and underlying mechanism in melanoma (SKCM) overall survival (OS). In this study, we used a comprehensive approach combining bioinformatics tools, including miRNA estimation, quantitative real-time polymerase chain reaction (qRT-PCR) of miRNAs, E3 ligase estimation, STRING analysis, TIMER analysis, examination of associated upstream modulators, protein-protein interaction (PPI) analysis, as well as retrospective and survival analyses, alongside clinical sample validation. These methods were used to investigate the content of BYSL, its methylation status, its relation to patient outcome, and its immunologic significance in tumors. Our findings revealed that BYSL expression is negatively regulated by BYSL methylation. Analysis of 468 cases of SKCM RNA sequencing samples demonstrated that enhanced BYSL expression was associated with higher tumor grade. We identified several miRNAs, namely hsa-miR-146b-3p, hsa-miR-342-3p, hsa-miR-511-5p, hsa-miR-3690, and hsa-miR-193a-5p, which showed a strong association with BYSL levels. Furthermore, we predicted the E3 ubiquitin ligase of BYSL and identified CBL, FBXW7, FZR1, KLHL3, and MARCH1 as potential modulators of BYSL. Through our investigation, we discovered that PNO1, RIOK2, TSR1, WDR3, and NOB1 proteins were strongly associated with BYSL expression. In addition, we found a close association between BYSL levels and certain immune cells, particularly dendritic cells (DCs). Notably, we observed a significant negative correlation between miR-146b-3p and BYSL mRNA expression in SKCM sera samples. Collectively, based on the previously shown evidences, BYSL can serve as a robust bioindicator of SKCM patient prognosis, and it potentially contributes to immune cell invasion in SKCM.

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来源期刊
Journal of Immunotherapy
Journal of Immunotherapy 医学-免疫学
CiteScore
6.90
自引率
0.00%
发文量
79
审稿时长
6-12 weeks
期刊介绍: Journal of Immunotherapy features rapid publication of articles on immunomodulators, lymphokines, antibodies, cells, and cell products in cancer biology and therapy. Laboratory and preclinical studies, as well as investigative clinical reports, are presented. The journal emphasizes basic mechanisms and methods for the rapid transfer of technology from the laboratory to the clinic. JIT contains full-length articles, review articles, and short communications.
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