用胃蛋白酶和胰蛋白酶酶解浓缩叶蛋白获得的菠萝叶蛋白水解物:分子量、细胞毒性、抗增殖活性和氧化应激。

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-01 Epub Date: 2024-07-10 DOI:10.1007/s11130-024-01203-9
Carolina Calderón-Chiu, Juan Arturo Ragazzo-Sánchez, Armando Ordaz-Hernández, Mayra Herrera-Martínez
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引用次数: 0

摘要

用胃肠道酶对浓缩叶蛋白进行酶水解得到的菠萝叶蛋白水解物显示出良好的技术功能特性和较高的抗氧化能力。然而,其分子量、抗增殖活性、细胞毒性和减少活性氧(ROS)的能力仍是未知数。因此,本研究旨在评估在不同水解时间(30-240 分钟)下用胃蛋白酶和胰蛋白酶进行酶水解得到的菠萝叶蛋白水解物对分子量、细胞毒性、抗癌细胞增殖以及减少 H2O2- 诱导的 HaCaT 细胞中活性氧的影响。电泳图谱显示,H-Pep 含有分子量在 25 - 20 kDa 之间的肽。同时,H-Pan 是由分子量在 25 - 20 kDa 之间的产物组成,H-Pep-60 分钟可使 2O2 受激的 HaCaT 细胞在 250 µg/mL 浓度下减少 25% 的 ROS(P
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Jackfruit Leaf Protein Hydrolysates Obtained by Enzymatic Hydrolysis of Leaf Protein Concentrate with Pepsin and Pancreatin: Molecular Weight, Cytotoxicity, Antiproliferative Activity, and Oxidative Stress.

Jackfruit leaf protein hydrolysates obtained from the enzymatic hydrolysis of leaf protein concentrate with gastrointestinal enzymes have shown good techno-functional properties and high antioxidant capacity. However, molecular weight, antiproliferative activity, cytotoxicity and the ability to reduce reactive oxygen species (ROS) are still unknown. Therefore, this study aimed to evaluate the effect of jackfruit leaf protein hydrolysates obtained by enzymatic hydrolysis with pepsin and pancreatin at different hydrolysis times (30-240 min) on molecular weights, cytotoxicity, antiproliferation of cancer cells, and the reduction of reactive oxygen species in H2O2-induced HaCaT cells. The electrophoretic profile indicated that H-Pep contains peptides with molecular weights between 25 - 20 kDa. Meanwhile, H-Pan is composed of molecular weight products between 25 - 20 kDa and < 20 kDa. H-Pan and H-Pep (125-500 µg/mL) did not show significant cytotoxicity on HaCaT (human keratinocytes) and J774A.1 (murine macrophage cells). Antiproliferative activity was achieved in human cervical, ovarian, and liver cancer cells. H-Pan-240 min (1000 µg/mL) reduced the cell viability of cervical cancer cells by 23% while H-Pan-60 min significantly reduced cell viability of ovarian and liver cancer cells by 14.5 (500 µg/mL) and 17% (1000 µg/mL), respectively (P < 0.05). The protective effect against oxidative stress on H2O2-stressed HaCaT cells was obtained with H-Pep-60 min, which reduced 25% of ROS at 250 µg/mL (P < 0.05). The findings demonstrate the safe use of green biomass as a source of plant protein hydrolysates.

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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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