感染不同戊型肝炎病毒株的无特异性病原体小猪的跨种传播和组织病理学变异。

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES Veterinary Research Pub Date : 2024-07-09 DOI:10.1186/s13567-024-01337-3
Soontag Jung, Daseul Yeo, Dong-Joo Seo, In-Soo Choi, Changsun Choi
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引用次数: 0

摘要

戊型肝炎病毒(HEV)是全球病毒性肝炎的主要病因。猪是 HEV 基因型 3 的天然宿主,也是 HEV 的主要储库。随着 HEV 基因型 3 宿主范围的扩大,不同物种的 HEV 通过猪传播给人类的可能性也在增加。我们通过让小猪感染猪 HEV(swHEV)、兔 HEV(rbHEV)和人 HEV(huHEV),并检查它们的组织病理学特征和在各器官中的分布情况,研究了 HEV 的潜在跨物种传播。15 只无特定病原体的尤卡坦小型猪分别感染了猪 HEV、兔 HEV、人 HEV 或模拟对照组。在本研究中,我们分析了为期七周的粪便脱落、病毒血症和血清学参数。结果表明,与非 swHEV 相比,swHEV 表现出更强的脱落和病毒血症。只有 swHEV 会影响血清学参数,这表明菌株之间存在特异性差异。组织病理学检查显示,感染每种 HEV 毒株后,肝脏、胰腺、肠道和淋巴组织都会出现不同的形态。值得注意的是,所有三种 HEV 都会诱发胰腺的组织病理学变化,这支持了 HEV 与急性胰腺炎的关联。我们的研究结果还发现骨骼肌是 HEV 抗原存在的部位,这表明 HEV 可能与肌炎有关。总之,这项研究为了解不同 HEV 株系在小型猪体内的感染动态提供了宝贵的见解,强调了病毒学、血清学和组织学参数方面的株系特异性变化。观察到的感染动力学和组织滋养性差异将有助于我们了解 HEV 的发病机制和跨物种传播的可能性。
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Cross-species transmission and histopathological variation in specific-pathogen-free minipigs infected with different hepatitis E virus strains.

Hepatitis E virus (HEV) is a major cause of viral hepatitis worldwide. Pigs are the natural host of HEV genotype 3 and the main reservoir of HEV. As the host range of HEV genotype 3 expands, the possibility that HEV from various species can be transmitted to humans via pigs is increasing. We investigated the potential cross-species transmission of HEV by infecting minipigs with swine HEV (swHEV), rabbit HEV (rbHEV), and human HEV (huHEV) and examining their histopathological characteristics and distribution in various organs. Fifteen specific-pathogen-free Yucatan minipigs were infected with swHEV, rbHEV, huHEV, or a mock control. In the present study, we analysed faecal shedding, viremia, and serological parameters over a seven-week period. Our results indicated that swHEV exhibited more robust shedding and viremia than non-swHEVs. Only swHEV affected the serological parameters, suggesting strain-specific differences. Histopathological examination revealed distinct patterns in the liver, pancreas, intestine, and lymphoid tissues after infection with each HEV strain. Notably, all three HEVs induced histopathological changes in the pancreas, supporting the association of HEVs with acute pancreatitis. Our results also identified skeletal muscle as a site of HEV antigen presence, suggesting a potential link to myositis. In conclusion, this study provides valuable insights into the infection dynamics of different HEV strains in minipigs, emphasizing the strain-specific variations in virological, serological, and histological parameters. The observed differences in infection kinetics and tissue tropism will contribute to our understanding of HEV pathogenesis and the potential for cross-species transmission.

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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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