利用 UPLC-Q-Orbitrap-MS 快速鉴定 Cinnamomi ramulus 中的各种化学成分。

IF 1.9 3区 化学 Q3 BIOCHEMICAL RESEARCH METHODS Journal of Mass Spectrometry Pub Date : 2024-07-11 DOI:10.1002/jms.5069
Lexin Shu, Sitong Liu, Fangfang Zhang, Huixin Qiu, Shumin Zhang, Jun Qian, Yanyan Xu, Yanru Deng, Yuming Wang, Yubo Li
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引用次数: 0

摘要

桂枝是一种历史悠久的常用中药材。临床上常用于治疗外感风寒疾病,但其化学成分仍有待研究。本研究采用75%乙醇提取CR,并利用UPLC-Q-Orbitrap-MS结合数据后处理方法鉴定提取物中的化学成分。通过该技术,可对 CR 中的成分进行分离和准确鉴定。共鉴定出 61 种化合物,其中包括 14 种简单的苯丙类化合物、3 种香豆素类化合物、5 种木脂素类化合物、14 种黄酮类化合物、10 种苯甲酸类化合物、8 种有机酸类化合物和 7 种其他化合物。该研究证实了这些化合物在 CR 中的存在,并推测了每种化合物的裂解途径,丰富了质谱数据和裂解规则。本研究可为 CR 及其他研究提供参考。
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Rapid identification of various chemical components in Cinnamomi ramulus by UPLC-Q-Orbitrap-MS

Cinnamomi ramulus (CR) is a common Chinese herbal medicine with a long history. It is often used to treat exogenous wind-cold diseases in clinic, but its chemical compositions remain to be studied. In this study, CR was extracted with 75% ethanol, and UPLC-Q-Orbitrap-MS combined with data post-processing method was used to identify the chemical components in the extract. Through this technology, the components in CR can be separated and accurately identified. A total of 61 compounds were identified, including 14 simple phenylpropanoids, 3 coumarins, 5 lignans, 14 flavonoids, 10 benzoic acids, 8 organic acids, and 7 others. This study confirmed the existence of these compounds in CR and speculated the cleavage pathways of each compound, which enriched the mass spectrometry data and cleavage rules. This study can provide a reference for CR and other research.

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来源期刊
Journal of Mass Spectrometry
Journal of Mass Spectrometry 化学-光谱学
CiteScore
5.10
自引率
0.00%
发文量
84
审稿时长
1.5 months
期刊介绍: The Journal of Mass Spectrometry publishes papers on a broad range of topics of interest to scientists working in both fundamental and applied areas involving the study of gaseous ions. The aim of JMS is to serve the scientific community with information provided and arranged to help senior investigators to better stay abreast of new discoveries and studies in their own field, to make them aware of events and developments in associated fields, and to provide students and newcomers the basic tools with which to learn fundamental and applied aspects of mass spectrometry.
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