人耳膜、脑脊液和血液中的 Prestin 作为听力损失的生物标志物

IF 2.6 3区 医学 Q1 OTORHINOLARYNGOLOGY Otolaryngology- Head and Neck Surgery Pub Date : 2024-12-01 Epub Date: 2024-07-11 DOI:10.1002/ohn.895
Anselm Joseph Gadenstaetter, Paul Emmerich Krumpoeck, Alice Barbara Auinger, Erdem Yildiz, Aldine Tu, Christian Matula, Christoph Arnoldner, Lukas David Landegger
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引用次数: 0

摘要

目的测定人血、脑脊液(CSF)和耳周液(PL)中预素的浓度,并评估其作为感音神经性听力损失(SNHL)临床生物标志物的适宜性:研究设计:42 名内耳道肿瘤或耳蜗内肿瘤患者在接受迷宫或中窝肿瘤切除术时,在术中采集了人体血液、CSF 和 PL 样本。使用酶联免疫吸附法测定普瑞斯汀的浓度,并进行线性回归分析,研究其与听力和前庭测试结果的关系:结果42名研究参与者(26名女性,平均±标准差年龄为52.7±12.5岁)血液样本中的预素浓度中位数为1.32(四分位数间距,IQR,0.71-1.99)纳克/毫升。脑脊液预素水平明显更高,为 4.73(IQR,2.45-14.03)纳克/毫升(P = .005)。与血液(P = .01)和脑脊液(P = .03)水平相比,PL prestin 浓度明显更高,为 84.74(IQR,38.95-122.00)纳克/毫升。线性回归分析表明,CSF 预激蛋白浓度与术前听力水平(纯音平均值和单词识别;P = .008,R2 = 0.1894;P = .03,R2 = 0.1857)有显著相关性,但与血液或 PL 水平无相关性:这项研究的结果凸显了预素水平的不稳定性,并首次揭示了这种潜在生物标志物在血液以外的体液中的浓度。未来的研究应全面评估不同病因引起的 SNHL 的人体预激素水平、预激素的自然平衡和全身循环以及耳蜗创伤后的时间动态。最后,在考虑这一诊断技术的潜在转化实施之前,迫切需要临床认可的预素检测试剂盒。
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Prestin in Human Perilymph, Cerebrospinal Fluid, and Blood as a Biomarker for Hearing Loss.

Objective: Determining the concentration of prestin in human blood, cerebrospinal fluid (CSF), and perilymph (PL), and evaluating its suitability as a clinical biomarker for sensori-neural hearing loss (SNHL).

Study design: Human blood, CSF, and PL samples were intraoperatively collected from 42 patients with tumors of the internal auditory canal or with intracochlear tumors undergoing translabyrinthine or middle fossa tumor removal. Prestin concentration was measured using enzyme-linked immunosorbent assay and linear regression analyses were performed to investigate its associations with audiological as well as vestibular test results.

Setting: Tertiary referral center.

Results: The median prestin concentration in blood samples of the 42 study participants (26 women, mean ± standard deviation age, 52.7 ± 12.5 years) was 1.32 (interquartile range, IQR, 0.71-1.99) ng/mL. CSF prestin levels were significantly higher with 4.73 (IQR, 2.45-14.03) ng/mL (P = .005). With 84.74 (IQR, 38.95-122.00) ng/mL, PL prestin concentration was significantly higher compared to blood (P = .01) and CSF (P = .03) levels. Linear regression analyses showed significant associations of CSF prestin concentration with preoperative hearing levels (pure-tone average and word recognition; P = .008, R2 = 0.1894; P = .03, R2 = 0.1857), but no correlations with blood or PL levels.

Conclusion and relevance: This study's findings highlight the volatile nature of prestin levels and provide the first insights into this potential biomarker's concentrations in body fluids apart from blood. Future investigations should comprehensively assess human prestin levels with different etiologies of SNHL, prestin's natural homeostasis and systemic circulation, and its temporal dynamics after cochlear trauma. Finally, clinically approved detection kits for prestin are urgently required prior to considering a potential translational implementation of this diagnostic technique.

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来源期刊
Otolaryngology- Head and Neck Surgery
Otolaryngology- Head and Neck Surgery 医学-耳鼻喉科学
CiteScore
6.70
自引率
2.90%
发文量
250
审稿时长
2-4 weeks
期刊介绍: Otolaryngology–Head and Neck Surgery (OTO-HNS) is the official peer-reviewed publication of the American Academy of Otolaryngology–Head and Neck Surgery Foundation. The mission of Otolaryngology–Head and Neck Surgery is to publish contemporary, ethical, clinically relevant information in otolaryngology, head and neck surgery (ear, nose, throat, head, and neck disorders) that can be used by otolaryngologists, clinicians, scientists, and specialists to improve patient care and public health.
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