银纳米粒子 (AgNPs) 的合成及其对多重耐药菌的抗菌效果。

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL Bio-medical materials and engineering Pub Date : 2024-01-01 DOI:10.3233/BME-240034
Na Xie
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引用次数: 0

摘要

背景:多重耐药菌(MDR)这一全球性问题的出现与抗生素的不当使用密切相关,因此迫切需要在医疗卫生领域进行抗菌创新。银纳米粒子(AgNPs)因其独特的物理和化学性质而显示出巨大的抗菌潜力。通过精确调节银纳米粒子的形态、尺寸和表面性质,可以有效增强银纳米粒子的抗菌性能,使其成为具有巨大发展潜力的新一代抗菌材料:检测 AgNPs 对 MDR 的抑制作用,为新型抗菌剂的研发提供更多可能:方法:通过氧化还原反应促进AgNPs的形成;肉汤微稀释法测定AgNPs对细菌的最小抑菌浓度(MIC);平板计数法评价AgNPs对多重耐药菌的杀灭效果;水晶紫染色法评价AgNPs对生物膜构建的抑制作用;通过逐渐增加AgNPs的浓度研究细菌的耐药性;CCK-8法检测AgNPs对细胞的毒性:结果:AgNPs对多种耐药菌有明显的杀菌作用。接触 AgNPs 溶液 12 小时后,大肠杆菌数量急剧下降,16 小时后金黄色葡萄球菌基本被消灭。特别是,AgNPs 对革兰氏阴性菌的抑制作用更强。此外,AgNPs 还能有效阻止细菌生物膜的形成,其抑制作用随 AgNPs 溶液浓度的增加而增强。长期使用 AgNPs,细菌对其产生耐药性的速度较慢。从安全性角度来看,AgNPs 对生物体无有害影响,具有生物安全性:结论:AgNPs 可抑制 MDR,对革兰氏阴性菌的抑菌能力高于革兰氏阳性菌。结论:AgNPs 可抑制 MDR,对革兰氏阴性菌的抑菌能力高于革兰氏阳性菌,还可抑制细菌生物膜的形成,避免耐药性,降低细胞毒性。
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Synthesis and antibacterial effects of silver nanoparticles (AgNPs) against multi-drug resistant bacteria.

Background: The emergence of the global problem of multi-drug resistant bacteria (MDR) is closely related to the improper use of antibiotics, which gives birth to an urgent need for antimicrobial innovation in the medical and health field. Silver nanoparticles (AgNPs) show significant antibacterial potential because of their unique physical and chemical properties. By accurately regulating the morphology, size and surface properties of AgNPs, the antibacterial properties of AgNPs can be effectively enhanced and become a next generation antibacterial material with great development potential.

Objective: The detection of the inhibitory effect of AgNPs on MDR provides more possibilities for the research and development of new antimicrobial agents.

Methods: Promote the formation of AgNPs by redox reaction; determine the minimum inhibitory concentration (MIC) of AgNPs to bacteria by broth microdilution method; evaluate the killing efficacy of AgNPs against multi-drug-resistant bacteria by plate counting; evaluate the inhibitory effect of AgNPs on biofilm construction by crystal violet staining; study the drug resistance of bacteria by gradually increasing the concentration of AgNPs; and detect the toxicity of AgNPs to cells by CCK-8 method.

Results: AgNPs has a significant bactericidal effect on a variety of drug-resistant bacteria. After exposure to AgNPs solution for 12 hours, the number of E. coli decreased sharply, and S. aureus was basically eliminated after 16 hours. In particular, AgNPs showed stronger inhibition against Gram-negative bacteria. In addition, AgNPs can effectively hinder the formation of bacterial biofilm, and its inhibitory effect increases with the increase of AgNPs solution concentration. When AgNPs is used for a long time, the development of bacterial resistance to it is slow. From the point of view of safety, AgNPs has no harmful effects on organisms and has biosafety.

Conclusion: AgNPs can inhibit MDR, and the bacteriostatic ability of Gram-negative bacteria is higher than that of Gram-positive bacteria. It can also inhibit the formation of bacterial biofilm, avoid drug resistance and reduce cytotoxicity.

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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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